Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma

  • Authors:
    • Yi Tang
    • Ya-Lan Wang
    • Lian Yang
    • Jian-Xia Xu
    • Wei Xiong
    • Ming Xiao
    • Ming Li
  • View Affiliations

  • Published online on: May 8, 2013     https://doi.org/10.3892/ijmm.2013.1370
  • Pages: 130-136
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Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) which mediates poly-ADP-ribosylation, has been extensively investigated in carcinoma compared to arginine ADP-ribosyltransferase 1 (ART1), which mediates mono‑ADP‑ribosylation. Previous studies have demonstrated that these enzymes promote proliferation and tumor development in colon carcinoma. However, whether there is any association between PARP-1 and ATR1 in colon carcinoma, remains unelucidated. In this study, using immunohistochemical analysis, we detected a higher expression of PARP-1 and ART1 in 63 samples from patients with colon carcinoma compared to 10 samples of normal colonic mucosa; our results revealed a positive correlation between the expression of PARP-1 and ART1 in the 63 human colon carcinoma tissue samples. To determine the correlation between PARP-1 and ART1, inhibitors of PARP-1 and ART1 and lentivirus vector‑mediated ART1 short‑hairpin RNA (shRNA) were used to culture CT26 murine colon adenocarcinoma cells separately. Using double‑label immunofluorescence assay, we detected the expression of PARP-1 in the CT26 cells, which was decreased following treatment with 5‑aminoisoquinolinone (5-AIQ, a PARP-1 inhibitor) or meta‑iodobenzylguanidine (MIBG, an ART1 inhibitor). However, the expression of ART1 only decreased when the CT26 cells were treated with MIBG. Furthermore, our results demonstrated that silencing ART1 inhibited PARP‑1 expression by decreasing the expression of nuclear factor-κB (NF-κB), inhibiting ras homolog A (RhoA). Hence, our data demonstrate the positive correlation between ART1 and PARP-1; the inhibition of ART1 activity downregulates PARP-1 expression by decreasing the activity of NF-κB in CT26 colon carcinoma cells.
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July 2013
Volume 32 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tang Y, Wang Y, Yang L, Xu J, Xiong W, Xiao M and Li M: Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma. Int J Mol Med 32: 130-136, 2013.
APA
Tang, Y., Wang, Y., Yang, L., Xu, J., Xiong, W., Xiao, M., & Li, M. (2013). Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma. International Journal of Molecular Medicine, 32, 130-136. https://doi.org/10.3892/ijmm.2013.1370
MLA
Tang, Y., Wang, Y., Yang, L., Xu, J., Xiong, W., Xiao, M., Li, M."Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma". International Journal of Molecular Medicine 32.1 (2013): 130-136.
Chicago
Tang, Y., Wang, Y., Yang, L., Xu, J., Xiong, W., Xiao, M., Li, M."Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma". International Journal of Molecular Medicine 32, no. 1 (2013): 130-136. https://doi.org/10.3892/ijmm.2013.1370