miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1

Tang,Haosha; Yao,Liangqing; Tao,Xiang; Yu,Yi; Chen,Mo; Zhang,Rong; Xu,Congjian

doi:10.3892/ijmm.2013.1400

miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1

Authors:
- Haosha Tang
- Liangqing Yao
- Xiang Tao
- Yi Yu
- Mo Chen
- Rong Zhang
- Congjian Xu
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Affiliations: Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China, Department of Pathology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China, Department of Obstetrics and Gynecology, Fengxian District Central Hospital, Shanghai 201400, P.R. China
Published online on: May 30, 2013 https://doi.org/10.3892/ijmm.2013.1400
Pages: 381-388

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Abstract

microRNAs (miRNAs) are important regulators of gene expression during tumorigenesis. The downregulation of microRNA-9 (miR-9) has been reported in ovarian serous carcinoma (OSC), indicating a role for miR-9 in this type of cancer. In this study, we investigated the biological significance of miR-9 in OSC in vitro. Using 3 OSC cell lines, SKOV3, CAOV3 and OVCAR3, which underexpresss miR-9, we demonstrate that the exogenous miR-9 transfection inhibits OSC cell proliferation, migration and invasion. In addition, we demonstrate that the focal adhesion protein, talin 1 (TLN1), whose expression has been associated with OSC development and progression to metastasis, is a direct target of miR-9. TLN1 knockdown mimicked the effects of miR-9 overexpression. Moreover, the activation of the TLN1-modulated FAK/AKT pathway was inhibited by the increased miR-9 levels. These results suggest that miR-9 plays a role as a tumor suppressor in OSC by suppressing TLN1 expression.

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