Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis

  • Authors:
    • Shuang Liang
    • Wei-Wei Su
    • Yong-Gang Wang
    • Wei Peng
    • Yi-Chu Nie
    • Pei-Bo Li
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  • Published online on: June 11, 2013     https://doi.org/10.3892/ijmm.2013.1414
  • Pages: 323-330
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Abstract

Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum. However, whether Q7R is one of the active ingredients responsible for the hepatopreventive effects of Hypericum japonicum has not yet been ascertained. Thus, the aim of the present study was to elucidate whether Q7R attenuates apoptosis induced by glycochenodeoxycholic acid (GCDC) in vitro, and to elucidate the mechanisms involved. L-02 human normal liver cells were pre-incubated with 0, 50, 100 and 200 µM Q7R for 30 min and then exposed to 100 µM GCDC for the indicated periods of time. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was performed to examine cell viability. Apoptosis was evaluated by Hoechst 33258 staining and Annexin V-FITC/PI double staining. Intracellular reactive oxygen species (ROS) were detected by flow cytometry using the oxidation-sensitive fluorescent probe, DCFH-DA. The assay for glutathione (GSH) was performed using a GSH detection kit. Intracellular Ca2+ concentration was evaluated using a confocal laser scanning microscope with Fluo-3 as the Ca2+ probe and mitochondrial membrane potential (Δψm) was measured by rhodamine 123 (Rh123) fluorescence. Q7R attenuated the GCDC-induced reduction in cell viability and the high apoptotic rate. Moreover, Q7R protected the L-02 cells from ROS overproduction, GSH depletion, intracellular Ca2+ accumulation and Δψm decrease induced by GCDC. These results suggest that Q7R attenuates L-02 cell injury induced by GCDC, possibly by inhibiting the overproduction of ROS, GSH depletion, intracellular Ca2+ accumulation and Δψm decrease, thereby minimizing L-02 cell apoptosis.
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August 2013
Volume 32 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liang S, Su W, Wang Y, Peng W, Nie Y and Li P: Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. Int J Mol Med 32: 323-330, 2013.
APA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., & Li, P. (2013). Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis. International Journal of Molecular Medicine, 32, 323-330. https://doi.org/10.3892/ijmm.2013.1414
MLA
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32.2 (2013): 323-330.
Chicago
Liang, S., Su, W., Wang, Y., Peng, W., Nie, Y., Li, P."Effect of quercetin 7-rhamnoside on glycochenodeoxycholic acid-induced L-02 human normal liver cell apoptosis". International Journal of Molecular Medicine 32, no. 2 (2013): 323-330. https://doi.org/10.3892/ijmm.2013.1414