Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest

  • Authors:
    • Shu-Kai Qiao
    • Han-Yun Ren
    • Yong-Jin Shi
    • Wei Liu
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  • Published online on: October 8, 2013     https://doi.org/10.3892/ijmm.2013.1518
  • Pages: 1373-1379
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Abstract

The human cervical cancer oncogene (HCCR2) has been found to be overexpressed in a variety of human malignant tumors cells, and its function is related to cell cycle progression and survival. However, the molecular mechanisms of action of HCCR2 in leukemia remain unclear. In this study, we used the RNA interference strategy to investigate the effects of HCCR2 knockdown in the K562 leukemia cell line, and to explore the potential mechanisms involved. Following transfection with small interfering RNA (siRNA) targeting HCCR2 (HCCR2-siRNA), we examined the effects of HCCR2 knockdown on cell morphology, cell proliferation, cell cycle progression and apoptosis in K562 cells. Morphological changes were evaluated by Wright-Giemsa staining. Cell cycle progression and apoptosis were measured by flow cytometry. The expression levels of genes related to the cell cycle and apoptosis were detected by quantitative RT-PCR (qRT-PCR) and western blot analysis. HCCR2 expression at the mRNA and protein level was significantly decreased following transfection with plasmids expressing HCCR2-siRNA. Silencing HCCR2 expression significantly suppressed cell proliferation, induced G1 cell cycle arrest and promoted the apoptosis of K562 cells. Additionally, we found that the expression of Bax, p53 and p21 was significantly increased, while Bcl-2 expression was significantly decreased in the HCCR2-siRNA-transfected cells. However, the expression of p27 was not affected. These results suggest that the HCCR2 gene plays an important role in the tumorigenesis of leukemia, thus making it an attractive therapeutic target for acute leukemia.
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December 2013
Volume 32 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qiao S, Ren H, Shi Y and Liu W: Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest. Int J Mol Med 32: 1373-1379, 2013.
APA
Qiao, S., Ren, H., Shi, Y., & Liu, W. (2013). Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest. International Journal of Molecular Medicine, 32, 1373-1379. https://doi.org/10.3892/ijmm.2013.1518
MLA
Qiao, S., Ren, H., Shi, Y., Liu, W."Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest". International Journal of Molecular Medicine 32.6 (2013): 1373-1379.
Chicago
Qiao, S., Ren, H., Shi, Y., Liu, W."Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest". International Journal of Molecular Medicine 32, no. 6 (2013): 1373-1379. https://doi.org/10.3892/ijmm.2013.1518