P7 peptides targeting bFGF sensitize colorectal cancer cells to CPT-11

  • Authors:
    • Wu Luo
    • Yonglin Yu
    • Ruixue Wang
    • Dan He
    • Cong Wang
    • Xiangfeng Zeng
    • Xilei Chen
    • Xiangpeng Tan
    • Tao Huang
    • Xiaoping Wu
  • View Affiliations

  • Published online on: November 4, 2013     https://doi.org/10.3892/ijmm.2013.1547
  • Pages: 194-200
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Abstract

The low survival rate of patients with colorectal cancer (CRC) is mainly due to the drug resistance of tumor cells to chemotherapeutic agents. It has been reported that basic fibroblast growth factor (bFGF) is an essential factor involved in the epigenetic mechanisms of drug resistance, which provides a novel potential target for improving the sensitivity of tumor cells to chemotherapeutic agents. In this study, we first demonstrate that a novel bFGF antagonist, peptide P7, previously isolated by phage display technology, reversed bFGF-induced resistance to irinotecan hydrochloride (CPT-11), and counteracted the anti-apoptotic effects of bFGF on CPT-11-treated HT-29 cells. Further experiments indicated that the inhibition of Akt activation, the suppression of bFGF internalization, the increase in the Bax to Bcl-2 ratio and the downregulation of cytokeratin 8 (CK8) by P7 may contribute to the counteracting of the anti-apoptotic effects of bFGF, and further reversal of bFGF-induced resistance to CPT-11. Our results suggest that peptide P7 may have therapeutic potential in CRC as a sensitizer to chemotherapeutic agents by targeting bFGF.
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2014-January
Volume 33 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Luo W, Yu Y, Wang R, He D, Wang C, Zeng X, Chen X, Tan X, Huang T, Wu X, Wu X, et al: P7 peptides targeting bFGF sensitize colorectal cancer cells to CPT-11. Int J Mol Med 33: 194-200, 2014.
APA
Luo, W., Yu, Y., Wang, R., He, D., Wang, C., Zeng, X. ... Wu, X. (2014). P7 peptides targeting bFGF sensitize colorectal cancer cells to CPT-11. International Journal of Molecular Medicine, 33, 194-200. https://doi.org/10.3892/ijmm.2013.1547
MLA
Luo, W., Yu, Y., Wang, R., He, D., Wang, C., Zeng, X., Chen, X., Tan, X., Huang, T., Wu, X."P7 peptides targeting bFGF sensitize colorectal cancer cells to CPT-11". International Journal of Molecular Medicine 33.1 (2014): 194-200.
Chicago
Luo, W., Yu, Y., Wang, R., He, D., Wang, C., Zeng, X., Chen, X., Tan, X., Huang, T., Wu, X."P7 peptides targeting bFGF sensitize colorectal cancer cells to CPT-11". International Journal of Molecular Medicine 33, no. 1 (2014): 194-200. https://doi.org/10.3892/ijmm.2013.1547