Oridonin protects HaCaT keratinocytes against hydrogen peroxide-induced oxidative stress by altering microRNA expression

Bae,Seunghee; Lee,Eun-Jin; Lee,Jae  Ho; Park,In-Chul; Lee,Su-Jae; Hahn,Hyung Jin; Ahn,Kyu Joong; An,Sungkwan; An,In-Sook; Cha,Hwa Jun

doi:10.3892/ijmm.2013.1561

Oridonin protects HaCaT keratinocytes against hydrogen peroxide-induced oxidative stress by altering microRNA expression

Authors:
- Seunghee Bae
- Eun-Jin Lee
- Jae Ho Lee
- In-Chul Park
- Su-Jae Lee
- Hyung Jin Hahn
- Kyu Joong Ahn
- Sungkwan An
- In-Sook An
- Hwa Jun Cha
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Affiliations: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul 100-380, Republic of Korea, Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea, Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
Published online on: November 19, 2013 https://doi.org/10.3892/ijmm.2013.1561
Pages: 185-193

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Abstract

microRNAs (miRNAs) have been shown to function as primary regulators of a variety of biological processes, including proliferation, differentiation and apoptosis in human keratinocytes. However, the biological significance of miRNAs in the defense against oxidative stress in keratinocytes remains to be elucidated. In this study, we demonstrate that oridonin, a diterpenoid compound isolated from Rabdosia rubescens with established antioxidant properties, protects HaCaT human keratinocytes from oxidative stress induced by exposure to hydrogen peroxide (H2O2). Our data demonstrate that low doses of oridonin (1-5 µM) protect keratinocytes against H2O2-induced apoptosis in a concentration- and time-dependent manner. Moreover, as shown by our results, oridonin markedly decreased H2O2-induced reactive oxygen species production in HaCaT cells. Oridonin mediated these effects by altering miRNA expression. Bioinformatics analysis identified several putative target genes of the differentially expressed miRNAs. Assessment of their gene ontology annotation revealed that these target genes are likely involved in cell growth and inhibition of apoptosis. Thus, the data from this study establish a role for miRNAs in mediating oridonin-induced protective effects against oxidative stress in human keratinocytes.

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