Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy

Zhang,Jiu-Cong; Xie,Fang; Yu,Xiao-Hui; Deng,Zhi-Yun; Wang,Yong; Liang,Ping; Sun,Li; Zhang,Fang-Xin

doi:10.3892/ijmm.2013.1584

Expression levels of P-glycoprotein in peripheral blood CD8+ T lymphocytes from HIV-1-infected patients on antiretroviral therapy

Authors:
- Jiu-Cong Zhang
- Fang Xie
- Xiao-Hui Yu
- Zhi-Yun Deng
- Yong Wang
- Ping Liang
- Li Sun
- Fang-Xin Zhang
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Affiliations: Department of Gastroenterology, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou, Gansu 730050, P.R. China, Department of Stress Medicine, Institute of Basic Medical Sciences, Beijing 100039, P.R. China, Department of Clinical Laboratory, Lanzhou General Hospital of Lanzhou Military Command, Lanzhou, Gansu 730050, P.R. China, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China
Published online on: December 11, 2013 https://doi.org/10.3892/ijmm.2013.1584
Pages: 431-440

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Abstract

In this study, we aimed to measure P-glycoprotein (P-gp) expression in CD8+ T lymphocytes of HIV-1-infected patients, to investigate how P-gp levels are affected by antiretroviral therapy (ART) in HIV-1 infection, and to assess the value of using P-gp expression to predict virologic response to ART. Peripheral blood mononuclear cells (PBMCs) were obtained from a cohort of HIV-1‑infected patients in China: 140 patients on ART, and 49 ART-naïve patients. We also enrolled 24 healthy blood donors as the controls. The expression levels of P-gp in CD8+ T cells of HIV-1-infected patients were evaluated by quantitative reverse transcription PCR, ELISA and flow cytometry. A high inter-individual variability was observed in the CD8+ T cells of both HIV-1-infected patients and healthy donors; however, the expression levels of P-gp were significantly higher in the HIV-1-infected group on ART compared to the ART-naïve group. The relative proportion of P-gp+CD8+ T cells inversely correlated with the blood CD4+ T cell count in the HIV-1‑infected patients on ART (r=-0.3343, P=0.0375). Groups of both good and poor responders showed significantly elevated levels of P-gp+CD8+ T cells. The percentage of P-gp+CD8+ T cells appeared to provide a sensitive estimate of the virologic response to ART compared to the CD4+ T cell count. Our results suggest that P-gp expression varies among HIV-1‑infected patients, but is significantly higher in HIV-1‑infected patients on ART. The overexpression of P-gp is involved in ART initiation during HIV-1 infection, and P-gp+CD8+ T cells may be an additional criterion for the evaluation of the antiretroviral response to ART.

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