Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

Cha,Hwa Jun; Lee,Kwang Sik; Lee,Ghang Tai; Lee,Kun Kook; Hong,Jin Tae; Lee,Sung Nae; Jang,Hyun Hee; Lee,Jae Ho; Park,In-Chul; Kim,Yu Ri; Ahn,Kyu Joong; Kwon,Seung Bin; An,In-Sook; An,Sungkwan; Bae,Seunghee

doi:10.3892/ijmm.2014.1647

Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

Authors:
- Hwa Jun Cha
- Kwang Sik Lee
- Ghang Tai Lee
- Kun Kook Lee
- Jin Tae Hong
- Sung Nae Lee
- Hyun Hee Jang
- Jae Ho Lee
- In-Chul Park
- Yu Ri Kim
- Kyu Joong Ahn
- Seung Bin Kwon
- In-Sook An
- Sungkwan An
- Seunghee Bae
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Affiliations: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea, Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea, College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk‑do 361-763, Republic of Korea, Department of Cosmetology, Kyung-In Women's College, Incheon 407-740, Republic of Korea, School of Art, Kyungbok University, Namyangju, Gyeonggi-do 472-948, Republic of Korea, Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul 100‑380, Republic of Korea, Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
Published online on: February 7, 2014 https://doi.org/10.3892/ijmm.2014.1647
Pages: 957-963

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Abstract

The aim of this study was to investigate the mechanisms by which troxerutin protects cells against ultraviolet B (UVB) radiation. First, we demonstrate that pre-treatment with troxerutin protects normal human dermal fibroblasts (nHDFs) against UVB-induced cytotoxicity. As shown by migration assay and DNA repair analysis, troxerutin increased cell migration and DNA repair activity in the nHDFs. Subsequently, we analyzed microRNA (miRNA) expression profiles in the nHDFs. miRNAs are 19- to 24-nucleotide (nt) non-coding RNA molecules that regulate the translation of target genes through RNA interference. In UVB-exposed cells, miRNAs act on crucial functions, such as apoptosis and cellular senescence. miRNA expression is significantly altered during the protective process induced by phytochemicals. Therefore, understanding changes that occur in miRNA expression profiles may help to elucidate the protective mechanisms of troxerutin. We identified 11 miRNAs that were significantly (>2-fold) upregulated and 12 that were significantly downregulated (>2-fold) following treatment of the nHDFs with troxerutin. In addition, we investigated the biological functions of these miRNAs through the prediction of miRNA targets and Gene Ontology analysis of the putative targets. Overall, our findings indicate that pre-treatment with troxerutin increases the viability of UVB-exposed nHDFs through the alteration of the miRNA expression profiles.

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