Estrogen is a novel regulator of Tnfaip1 in mouse hippocampus

Liu,Hui; Yang,Liping; Zhao,Yingchun; Zeng,Guihua; Wu,Yaosong; Chen,Yulong; Zhang,Jian; Zeng,Qingru

doi:10.3892/ijmm.2014.1742

Estrogen is a novel regulator of Tnfaip1 in mouse hippocampus

Authors:
- Hui Liu
- Liping Yang
- Yingchun Zhao
- Guihua Zeng
- Yaosong Wu
- Yulong Chen
- Jian Zhang
- Qingru Zeng
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Affiliations: Department of Life Science, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China, Department of Enviromental Science, Changsha Environmental Protection College, Changsha, Hunan 410004, P.R. China, Department of Biostatistics and Bioinformatics, Tulane University, New Orleans, LA 70112, USA, Laboratory of Molecular Biology, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 410208, P.R. China, Key Laboratory of Protein Chemistry and Developmental Biology, Ministry of Education of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan 410081, P.R. China
Published online on: April 11, 2014 https://doi.org/10.3892/ijmm.2014.1742
Pages: 219-227

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Abstract

Tumor necrosis factor‑induced protein 1 (Tnfaip1), also known as B12, has been previously identified as a tumor necrosis factor-α (TNF-α)-inducible protein and is involved in the cytokinesis signaling pathway, DNA synthesis, innate immunity, cell apoptosis, Alzheimer's disease (AD) and type 2 diabetic nephropathy. However, little is known regarding the expression of Tnfaip1 in various tissues or its accurate role in these physiological functions. The focus of this study was on Tnfaip1 expression in different tissues, with a high expression in mouse hippocampus being identified. The age- and gender‑related expression of Tnfaip1 in hippocampus was also investigated. The distribution of Tnfaip1 was mapped using fluorescent immunostaining. Although immunoactivity was found in the CA1, CA3 and DG subregions of the hippocampus in E17.5 and P6 mice, strong staining was only detected in the CA3 subregion in adult mice. These data suggested that Tnfaip1 expression in hippocampus may be regulated by estrogen. Further study showed that the expression of Tnfaip1 in the hippocampus was significantly increased in ovariectomized mice compared to Sham mice. In cultured primary hippocampal cells, Tnfaip1 showed different expression levels in different treatments of estrogen or estrogen receptor antagonists. Additional experiments demonstrated the existence of a binding site of ERβ in the Tnfaip1 promoter region, and that ERβ was able to upregulate Tnfaip1 expression. Our study identified a new regulatory factor and a primary regulatory mechanism of Tnfaip1 expression in hippocampus. Since both hippocampus and estrogen are crucial in AD, the results also showed a potential association between Tnfaip1 and hippocampal-related diseases, such as AD, which may be affected by the estrogen level.

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