Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts

Ryu,Jina; Park,Su-Jin; Kim,In-Hye; Choi,Youn Hee; Nam,Taek-Jeong

doi:10.3892/ijmm.2014.1815

Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts

Authors:
- Jina Ryu
- Su-Jin Park
- In-Hye Kim
- Youn Hee Choi
- Taek-Jeong Nam
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Affiliations: Department of Food and Life Science, Pukyong National University, Busan 608-737, Republic of Korea, Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea
Published online on: June 19, 2014 https://doi.org/10.3892/ijmm.2014.1815
Pages: 796-803
Copyright: © Ryu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The significant increase in life expectancy is closely related to the growing interest in the impact of aging on the function and appearance of the skin. Skin aging is influenced by several factors, and solar ultraviolet (UV) irradiation is considered one of the most important causes of skin photoaging. The aim of this study was to examine the anti-photoaging role of porphyra-334 from Porphyra (P.) yezoensis, a mycosporine-like amino acid (MAA), using high-performance liquid chromatography (HPLC), and electrospray ionization‑mass spectrometry (ESI-MS). In the present study, extracted UV‑absorbing compounds from P. yezoensis included palythine, asterina-330 and porphyra-334. Porphyra-334 was the most abundant MAA in P. yezoensis, and it was therefore used for conducting antiphotoaging experiments. The effect of porphyra-334 on the prevention of photoaging was investigated by measuring reactive oxygen species (ROS) production and matrix metalloproteinase (MMP) levels, as well as extracellular matrix (ECM) components and protein expression in UVA‑irradiated human skin fibroblasts. Porphyra-334 suppressed ROS production and the expression of MMPs following UVA irradiation, while increasing levels of ECM components, such as procollagen, type I collagen, elastin. These results suggest that porphyra-334 has various applications in cosmetics and toiletries because of its anti‑photoaging activities and may serve as a novel anti-aging agent.

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