Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non‑cancerous liver cells

  • Authors:
    • Yunjuan Zhang
    • Wei Duan
    • Lawrence Owusu
    • Dachang Wu
    • Yi Xin
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  • Published online on: October 31, 2014     https://doi.org/10.3892/ijmm.2014.1988
  • Pages: 117-124
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Abstract

Epigallocatechin‑3‑gallate (EGCG) is a constituent of green tea and has been associated with anticancer activity. In the present study, the inhibitory effect of EGCG on human hepatocellular cancer cells was examined by cell viability assay, in vitro apoptosis assay and cell cycle analysis. In addition, gene expression was measured to elucidate the molecular mechanisms of action of EGCG by mitochondrial membrane potential (MMP) determination and western blot analysis. We demonstrated that EGCG induced apoptosis, decreased mitochondrial membrane potential and promoted G0/G1 phase cell cycle arrest of HCCLM6 cells but not that of non-cancerous liver cells (HL-7702). The EGCG-induced apoptosis of HCCLM6 cells was associated with a significant decrease in Bcl-2 and NF-κB expression. In addition, the expression of Bax, p53, caspase-9 and caspase-3 increased, and cytochrome c was released. These results suggest that EGCG inhibits the progression of cancer through cytocidal activity and that it is a potential therapeutic compound for hepatocellular carcinoma (HCC).
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January-2015
Volume 35 Issue 1

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Spandidos Publications style
Zhang Y, Duan W, Owusu L, Wu D and Xin Y: Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non‑cancerous liver cells. Int J Mol Med 35: 117-124, 2015.
APA
Zhang, Y., Duan, W., Owusu, L., Wu, D., & Xin, Y. (2015). Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non‑cancerous liver cells. International Journal of Molecular Medicine, 35, 117-124. https://doi.org/10.3892/ijmm.2014.1988
MLA
Zhang, Y., Duan, W., Owusu, L., Wu, D., Xin, Y."Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non‑cancerous liver cells". International Journal of Molecular Medicine 35.1 (2015): 117-124.
Chicago
Zhang, Y., Duan, W., Owusu, L., Wu, D., Xin, Y."Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non‑cancerous liver cells". International Journal of Molecular Medicine 35, no. 1 (2015): 117-124. https://doi.org/10.3892/ijmm.2014.1988