Resveratrol exerts growth inhibitory effects on human SZ95 sebocytes through the inactivation of the PI3-K/Akt pathway

  • Authors:
    • So Young Kim
    • Moo Yeol Hyun
    • Kyung Chan Go
    • Christos C. Zouboulis
    • Beom Joon Kim
  • View Affiliations

  • Published online on: February 12, 2015     https://doi.org/10.3892/ijmm.2015.2098
  • Pages: 1042-1050
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Abstract

Resveratrol is known to be a naturally produced polyphenol that is able to reduce cell proliferation in several types of cancer cells and adipocytes. However, the antiproliferative effects of resveratrol on the growth of human sebocytes are not yet clear. In the present study, we investigated possible cellular pathways associated with these growth inhibitory effects on human SZ95 sebocytes. Our results revealed that resveratrol inhibited the proliferation of sebocytes, and that this resulted in disruption of the cell cycle. The inactivation of extracellular signal‑regulated protein kinase (ERK), Akt and peroxisome proliferator‑activated receptor (PPAR)‑γ was also shown to be involved in the inhibition of sebocyte growth by resveratrol. To examine the antiproliferative effects of resveratrol, we determined the levels of cell cycle control proteins. Resveratrol inhibited cyclin D1 synthesis, whereas it stimulated p21WAF1/CIP1 (p21) and p27KIP1 (p27) synthesis. In addition, we demonstrated that the resveratrol‑mediated cell cycle arrest resulted in an increase in the proportion of cells in the sub‑G0/G1 phase. Moreover, we found that the growth inhibitory effects of resveratrol were enhanced by treatment with LY294002 [a phosphatidylinositol 3‑kinase (PI3‑K) inhibitor] more so than by treatment with PD98059 (a MEK inhibitor), which indicates that resveratrol exerts its inhibitory effects on sebocyte proliferation through the inhibition of Akt. Linoleic acid (LA) is a well‑established lipid inducer in sebocytes and is known to stimulate sebocyte differentiation through the upregulation of PPAR‑γ. In this study, resveratrol was found to decrease the lipid content and PPAR‑γ expression during LA‑stimulated lipogenesis. Our results indicate that resveratrol plays a critical role in the inhibition of sebocyte growth through the inactivation of the Akt pathway. The present data suggest that resveratrol may be used as a therapeutic agent for the treatment of acne vulgaris.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim SY, Hyun MY, Go KC, Zouboulis CC and Kim BJ: Resveratrol exerts growth inhibitory effects on human SZ95 sebocytes through the inactivation of the PI3-K/Akt pathway. Int J Mol Med 35: 1042-1050, 2015.
APA
Kim, S.Y., Hyun, M.Y., Go, K.C., Zouboulis, C.C., & Kim, B.J. (2015). Resveratrol exerts growth inhibitory effects on human SZ95 sebocytes through the inactivation of the PI3-K/Akt pathway. International Journal of Molecular Medicine, 35, 1042-1050. https://doi.org/10.3892/ijmm.2015.2098
MLA
Kim, S. Y., Hyun, M. Y., Go, K. C., Zouboulis, C. C., Kim, B. J."Resveratrol exerts growth inhibitory effects on human SZ95 sebocytes through the inactivation of the PI3-K/Akt pathway". International Journal of Molecular Medicine 35.4 (2015): 1042-1050.
Chicago
Kim, S. Y., Hyun, M. Y., Go, K. C., Zouboulis, C. C., Kim, B. J."Resveratrol exerts growth inhibitory effects on human SZ95 sebocytes through the inactivation of the PI3-K/Akt pathway". International Journal of Molecular Medicine 35, no. 4 (2015): 1042-1050. https://doi.org/10.3892/ijmm.2015.2098