Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury

  • Authors:
    • Ju-Mi Hong
    • Ok-Kyoung Kwon
    • In-Sik Shin
    • Chan-Mi Jeon
    • Na-Rae Shin
    • Joongku Lee
    • Sang-Hong Park
    • Tran The Bach
    • Do Van Hai
    • Sei-Ryang Oh
    • Sang-Bae Han
    • Kyung-Seop Ahn
  • View Affiliations

  • Published online on: March 2, 2015     https://doi.org/10.3892/ijmm.2015.2117
  • Pages: 1403-1410
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Abstract

Canarium lyi C.D. Dai & Yakovlev (CL) is a member of the Anacardiaceae family. To the best of our knowledge, no studies on its anti-inflammatory effects have yet been reported. In the present study, we investigated the protective effects of CL on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-induced acute lung injury (ALI) mice. CL attenuated the production of LPS-stimulated inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and interleukin-6 (IL-6). Furthermore, CL suppressed phosphorylation of the inhibitor κB-α (IκB-α), p38, c-Jun terminal kinase (JNK) and extracellular signal-regulated kinase (ERK), as well as the translocation of the nuclear factor-κB (NF-κB) p65 subunit into the nucleus. For the in vivo efficacy, the effect of CL on a mouse model of LPS-induced acute lung injury was assessed. CL treatment of the mice significantly inhibited the inflammatory cell recruitment and pro-inflammatory cytokine production in bronchoalveolar lavage fluids (BALF). CL-treated mice also showed a marked inhibition of cyclooxygenase-2 (COX-2) and phosphorylation of IκB and p65. In addition, CL attenuated lung histopathological changes in LPS-induced ALI mice. In conclusion, our results suggest that CL is a potential therapeutic candidate for the treatment of inflammatory diseases, including pneumonia.
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May-2015
Volume 35 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hong J, Kwon O, Shin I, Jeon C, Shin N, Lee J, Park S, Bach TT, Hai DV, Oh S, Oh S, et al: Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury. Int J Mol Med 35: 1403-1410, 2015.
APA
Hong, J., Kwon, O., Shin, I., Jeon, C., Shin, N., Lee, J. ... Ahn, K. (2015). Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury. International Journal of Molecular Medicine, 35, 1403-1410. https://doi.org/10.3892/ijmm.2015.2117
MLA
Hong, J., Kwon, O., Shin, I., Jeon, C., Shin, N., Lee, J., Park, S., Bach, T. T., Hai, D. V., Oh, S., Han, S., Ahn, K."Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury". International Journal of Molecular Medicine 35.5 (2015): 1403-1410.
Chicago
Hong, J., Kwon, O., Shin, I., Jeon, C., Shin, N., Lee, J., Park, S., Bach, T. T., Hai, D. V., Oh, S., Han, S., Ahn, K."Anti-inflammatory effects of methanol extract of Canarium lyi C.D. Dai & Yakovlev in RAW 264.7 macrophages and a murine model of lipopolysaccharide-induced lung injury". International Journal of Molecular Medicine 35, no. 5 (2015): 1403-1410. https://doi.org/10.3892/ijmm.2015.2117