Open Access

Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells

  • Authors:
    • Bo Li
    • Do Sung Kim
    • Raj Kumar Yadav
    • Hyung Ryong Kim
    • Han Jung Chae
  • View Affiliations

  • Published online on: April 28, 2015     https://doi.org/10.3892/ijmm.2015.2199
  • Pages: 53-64
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Sulforaphane, a natural isothiocyanate compound found in cruciferous vegetables, has been shown to exert cardioprotective effects during ischemic heart injury. However, the effects of sulforaphane on cardiotoxicity induced by doxorubicin are unknown. Thus, in the present study, H9c2 rat myoblasts were pre-treated with sulforaphane and its effects on cardiotoxicity were then examined. The results revealed that the pre-treatment of H9c2 rat myoblasts with sulforaphane decreased the apoptotic cell number (as shown by trypan blue exclusion assay) and the expression of pro-apoptotic proteins (Bax, caspase-3 and cytochrome c; as shown by western blot analysis and immunostaining), as well as the doxorubicin-induced increase in mitochondrial membrane potential (measured by JC-1 assay). Furthermore, sulforaphane increased the mRNA and protein expression of heme oxygenase-1 (HO-1, measured by RT-qPCR), which consequently reduced the levels of reactive oxygen species (ROS, measured using MitoSOX Red reagent) in the mitochondria which were induced by doxorubicin. The cardioprotective effects of sulforaphane were found to be mediated by the activation of the Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor-2 (Nrf2)/antioxidant-responsive element (ARE) pathway, which in turn mediates the induction of HO-1. Taken together, the findings of this study demonstrate that sulforaphane prevents doxorubicin-induced oxidative stress and cell death in H9c2 cells through the induction of HO-1 expression.
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July-2015
Volume 36 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li B, Kim DS, Yadav RK, Kim HR and Chae HJ: Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med 36: 53-64, 2015.
APA
Li, B., Kim, D.S., Yadav, R.K., Kim, H.R., & Chae, H.J. (2015). Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. International Journal of Molecular Medicine, 36, 53-64. https://doi.org/10.3892/ijmm.2015.2199
MLA
Li, B., Kim, D. S., Yadav, R. K., Kim, H. R., Chae, H. J."Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells". International Journal of Molecular Medicine 36.1 (2015): 53-64.
Chicago
Li, B., Kim, D. S., Yadav, R. K., Kim, H. R., Chae, H. J."Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells". International Journal of Molecular Medicine 36, no. 1 (2015): 53-64. https://doi.org/10.3892/ijmm.2015.2199