Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Choi,Youn  Hee; Kim,Eun-Young; Mikami,Koji; Nam,Taek  Jeong

doi:10.3892/ijmm.2015.2253

Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death

Authors:
- Youn Hee Choi
- Eun-Young Kim
- Koji Mikami
- Taek Jeong Nam
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Affiliations: Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea, Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan
Published online on: June 19, 2015 https://doi.org/10.3892/ijmm.2015.2253
Pages: 369-376
Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In the present study, the chemoprotective effects of recombinant Pyropia yezoensis (P. yezoensis) protein 1 (PYP1) were examined in acetaminophen (APAP)-treated Chang liver cells. The analysis of P. yezoensis revealed the presence of both mature and immature variants of PYP1. PYP1s, designated as PYP1 (15 kDa), PYP1-AC (12 kDa) and PYP1-B (5 kDa), were successfully expressed in Escherichia coli, and their chemoprotective effects were then examined. In addition, a peptide of 11 residues (ALEGGKSSGGG), which is a common sequence at the N-terminus all of the PYP1s, was synthesized and examined. The effects of treatment with PYP1s and the synthetic peptide (SP) on cell proliferation were determined by MTS assay. Our results clearly demonstrated that treatment with all the PYP1s and SP significantly promoted the proliferation of Chang liver cells, protecting them against APAP. Thus, we concluded that recombinant PYP1s exert protective effects against injury to Chang liver cells.

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