Puerarin attenuates glucocorticoid-induced apoptosis of hFOB1.19 cells through the JNK- and Akt-mediated mitochondrial apoptotic pathways

Yu,Dongdong; Mu,Shuai; Zhao,Danyang; Wang,Guangbin; Chen,Zhiguang; Ren,Hongfei; Fu,Qin

doi:10.3892/ijmm.2015.2258

Puerarin attenuates glucocorticoid-induced apoptosis of hFOB1.19 cells through the JNK- and Akt-mediated mitochondrial apoptotic pathways

Authors:
- Dongdong Yu
- Shuai Mu
- Danyang Zhao
- Guangbin Wang
- Zhiguang Chen
- Hongfei Ren
- Qin Fu
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Affiliations: Department of Orthopedic Surgery, The Shengjing Hospital of China Medical University, Liaoning 110004, P.R. China
Published online on: June 23, 2015 https://doi.org/10.3892/ijmm.2015.2258
Pages: 345-354
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Puerarin is an active component of Pueraria lobata, which is a commonly used Chinese herbal medicine for the treatment of osteoporosis. The present study aimed to evaluate the osteoprotective effect of puerarin on glucocorticoid (GC)‑induced apoptosis of osteoblasts in vitro. The effects of puerarin on dexamethasone (DEX)‑induced cell apoptosis were assessed using enzyme‑linked immunosorbent assay and a terminal deoxynucleotidyl transferase dUTP nick‑end labeling assay, and found that the viability of hFOB1.19 cells was significantly increased following exposure to between 10‑6 and 10‑10 M puerarin, with a maximal anti‑apoptotic effect at a concentration of 10‑8 M. In addition, compared with the control group, puerarin upregulated the transcription and protein levels of B‑cell lymphoma‑2 and downregulated B‑cell‑associated X protein in the hFOB1.19 cells. Puerarin attenuated the DEX‑induced release of cytochrome c and cleavage of caspase‑3, and treatment with puerarin inhibited the c‑Jun N‑terminal kinase (JNK) pathway and activated the phosphoinositide 3‑kinase (PI3K)/Akt pathway in the hFOB1.19 cells. Furthermore, the Akt inhibitor, LY294002, partly eliminated the protective effect of puerarin on DEX‑induced apoptosis, and puerarin combined with the JNK inhibitor, SP600125, suppressed DEX‑induced apoptosis to a lesser extent than in the cells treated with SP600125 alone. These results suggested that the JNK and PI3K/Akt signaling pathways mediate the inhibitory effects of puerarin on apoptosis in the hFOB1.19 cells. In conclusion, puerarin prevented DEX‑induced apoptosis of hFOB1.19 cells via inhibition of the JNK pathway and activation of the PI3K/Akt signaling pathway in the cells, dependent on the mitochondrial apoptotic pathway. These results support puerarin as a promising target in the treatment of GC-induced osteoporosis.

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