Open Access

Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells

  • Authors:
    • Wei Zheng
    • Shi Wang
    • Jianguo Wang
    • Fang Jin
  • View Affiliations

  • Published online on: August 17, 2015     https://doi.org/10.3892/ijmm.2015.2314
  • Pages: 915-922
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the periodontitis-associated changes in the number, proliferation and differentiation potential of human periodontal ligament stem cells (PDLSCs). Cultures of human periodontal ligament cells (PDLCs) were established from healthy donors and donors with periodontitis. The numbers of stem cell were characterized using flow cytometry. PDLSCs were isolated from the PDLCs by immunomagnetic bead selection. Colony‑forming abilities, osteogenic and adipogenic potential, gene expression of cementoblast phenotype, alkaline phosphatase activity and in vivo differentiation capacities were then evaluated. Periodontitis caused an increase in the proliferation of PDLSCs and a decrease in the commitment to the osteoblast lineage. This is reflected by changes in the expression of osteoblast markers. When transplanted into immunocompromised mice, PDLSCs from the healthy donors exhibited the capacity to produce cementum PDL‑like structures, whereas, the inflammatory PDLSCs transplants predominantly formed connective tissues. In conclusion, the data from the present study suggest that periodontitis affects the proliferation and differentiation potential of human PDLSCs in vitro and in vivo.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 36 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zheng W, Wang S, Wang J and Jin F: Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells. Int J Mol Med 36: 915-922, 2015.
APA
Zheng, W., Wang, S., Wang, J., & Jin, F. (2015). Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells. International Journal of Molecular Medicine, 36, 915-922. https://doi.org/10.3892/ijmm.2015.2314
MLA
Zheng, W., Wang, S., Wang, J., Jin, F."Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells". International Journal of Molecular Medicine 36.4 (2015): 915-922.
Chicago
Zheng, W., Wang, S., Wang, J., Jin, F."Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells". International Journal of Molecular Medicine 36, no. 4 (2015): 915-922. https://doi.org/10.3892/ijmm.2015.2314