miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells

Song,Qiling; Zhong,Liang; Chen,Chu; Tang,Zuchuan; Liu,Hongxia; Zhou,Yiqin; Tang,Min; Zhou,Lan; Zuo,Guowei; Luo,Jinyong; Zhang,Yan; Shi,Qiong; Weng,Yaguang

doi:10.3892/ijmm.2015.2363

miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells

Authors:
- Qiling Song
- Liang Zhong
- Chu Chen
- Zuchuan Tang
- Hongxia Liu
- Yiqin Zhou
- Min Tang
- Lan Zhou
- Guowei Zuo
- Jinyong Luo
- Yan Zhang
- Qiong Shi
- Yaguang Weng
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Affiliations: Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, P.R. China
Published online on: October 9, 2015 https://doi.org/10.3892/ijmm.2015.2363
Pages: 1497-1506
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone morphogenetic proteins (BMPs), particularly BMP9, have been shown to promote the osteogenic differentiation of murine multilineage cells (MMCs) and to promote bone formation in bone diseases; however, the mechanisms involved remain poorly understood. MicroRNAs (miRNAs or miRs) have been proven to regulate mesenchymal stem cell (MSC) differentiation. In this study, we identified a novel mechanism that unravels the functional axis of a key miRNA (miR-21) which contributes to BMP9‑induced osteogenic differentiation. We screened differentially expressed miRNAs in MMCs during BMP9‑induced osteogenic differentiation and found that miR-21 was significantly upregulated by BMP9 during the osteogenesis of MMCs. Furthermore, miR-21 was confirmed to promote the osteogenic differentiation of the MMCs by suppressing Smad7, which negatively regulates the osteogenic differentiation of MMCs. The upregulation of miR-21 may promote the osteogenic differentiation of MMCs in synergy with BMP9. The findings of our study revealed a novel function of miR-21, and suggest that the overexpression of miR-21 contributes to bone formation by promoting BMP9‑induced osteogenic differentiation. Our data may provide a molecular basis for the development of novel therapeutic strategies to treat bone diseases, such as osteoporosis and other inflammatory bone diseases.

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