The mechanisms of substance P-mediated migration of bone marrow-derived mesenchymal stem cell-like ST2 cells

  • Authors:
    • Maria Jose Dubon
    • Ki-Sook Park
  • View Affiliations

  • Published online on: February 19, 2016     https://doi.org/10.3892/ijmm.2016.2496
  • Pages: 1105-1111
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Abstract

Substance P (SP) is known to induce the mobilization of bone marrow-derived mesenchymal stem cells (BM-MSCs) and thus participates in wound repair. However, the cellular and molecular mechanisms responsible for the SP-mediated migration of BM-MSCs were not fully understood. In the present study, we studied the molecular mechanisms that mediate the migration of the BM-derived MSC-like cell line ST2 in response to SP. Using a migration assay and western blot analysis, we noted that SP induced the chemotactic migration of ST2 cells through the intrinsic activation of extracellular signal-regulated kinases (ERKs) and protein kinase B (Akt), the phosphorylated expression levels of which were increased. We noted that Src is involved in the SP-mediated migration of ST2 cells and that focal adhesion kinase (FAK) was activated in the ST2 cells following SP treatment. Membrane ruffling increased in the ST2 cells after SP treatment, as was clearly demonstrated by immunocytochemical analysis. Importantly, using a blocking antibody against N-cadherin (GC-4), we studied cell migration and noted that SP mediated the migration of the ST2 cells through N-cadherin. The present study thus advanced our understanding of the mechanisms through which SP induces BM-MSC migration.
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April-2016
Volume 37 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Dubon MJ and Park K: The mechanisms of substance P-mediated migration of bone marrow-derived mesenchymal stem cell-like ST2 cells. Int J Mol Med 37: 1105-1111, 2016.
APA
Dubon, M.J., & Park, K. (2016). The mechanisms of substance P-mediated migration of bone marrow-derived mesenchymal stem cell-like ST2 cells. International Journal of Molecular Medicine, 37, 1105-1111. https://doi.org/10.3892/ijmm.2016.2496
MLA
Dubon, M. J., Park, K."The mechanisms of substance P-mediated migration of bone marrow-derived mesenchymal stem cell-like ST2 cells". International Journal of Molecular Medicine 37.4 (2016): 1105-1111.
Chicago
Dubon, M. J., Park, K."The mechanisms of substance P-mediated migration of bone marrow-derived mesenchymal stem cell-like ST2 cells". International Journal of Molecular Medicine 37, no. 4 (2016): 1105-1111. https://doi.org/10.3892/ijmm.2016.2496