Open Access

293 cells express both epithelial as well as mesenchymal cell adhesion molecules

  • Authors:
    • Masakazu Inada
    • Genya Izawa
    • Wakako Kobayashi
    • Masayuki Ozawa
  • View Affiliations

  • Published online on: April 18, 2016     https://doi.org/10.3892/ijmm.2016.2568
  • Pages: 1521-1527
  • Copyright: © Inada et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The 293 cell line, used extensively in various types of studies due to the ease with which these cells can be transfected, was thought to be derived by the transformation of primary cultures of human embryonic kidney cells with sheared adenovirus type 5 DNA. Although the 293 cells were assumed to originate from epithelial cells, the exact origin of these cells remains unknown. Previous attempts to characterize these cells combined immunostaining, immunoblot analysis and microarray analysis to demonstrate that 293 cells express neurofilament subunits, α-internexin, and several other proteins typically found in neurons. These findings raised the possibility that the 293 cell line may have originated from human neuronal lineage cells. Contrary to this suggestion, in this study, we found that the 293 cells expressed N-cadherin and vimentin, which are marker proteins expressed in mesenchymal cells. Furthermore, the 293 cells also expressed E-cadherin, cytokeratins 5/8 and desmoglein 2, which are epithelial cell markers. When the cells, primarily cultured from the kidneys of Clawn miniature swine and passaged 10-15 generations [termed porcine kidney epithelial (PKE) cells] were examined, they were found to be positive for the expression of both mesenchymal and epithelial markers. Thus, transformation by adenovirus was not necessary for the cells to express N-cadherin. Occludin and zonula occludens (ZO)-1, two components of tight junctions in epithelial and endothelial cells, were detected in the 293 and the PKE cells. Thus, the findings of the present study demonstrate that 293 cells retain several characteristics of epithelial cells.
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June-2016
Volume 37 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Inada M, Izawa G, Kobayashi W and Ozawa M: 293 cells express both epithelial as well as mesenchymal cell adhesion molecules. Int J Mol Med 37: 1521-1527, 2016.
APA
Inada, M., Izawa, G., Kobayashi, W., & Ozawa, M. (2016). 293 cells express both epithelial as well as mesenchymal cell adhesion molecules. International Journal of Molecular Medicine, 37, 1521-1527. https://doi.org/10.3892/ijmm.2016.2568
MLA
Inada, M., Izawa, G., Kobayashi, W., Ozawa, M."293 cells express both epithelial as well as mesenchymal cell adhesion molecules". International Journal of Molecular Medicine 37.6 (2016): 1521-1527.
Chicago
Inada, M., Izawa, G., Kobayashi, W., Ozawa, M."293 cells express both epithelial as well as mesenchymal cell adhesion molecules". International Journal of Molecular Medicine 37, no. 6 (2016): 1521-1527. https://doi.org/10.3892/ijmm.2016.2568