Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion

Yang,Xiaochen; Steinberg,Florian; Zhuang,Lei; Bessey,Ralph; Trueb,Beat

doi:10.3892/ijmm.2016.2601

Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion

Authors:
- Xiaochen Yang
- Florian Steinberg
- Lei Zhuang
- Ralph Bessey
- Beat Trueb
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Affiliations: Department of Clinical Research, University of Bern, CH-3008 Bern, Switzerland
Published online on: May 23, 2016 https://doi.org/10.3892/ijmm.2016.2601
Pages: 30-38
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fibroblast growth factor receptor (FGFR)-like protein 1 (FGFRL1) is the most recently discovered member of the FGFR family. Owing to the fact that it interacts with FGF ligands, but lacks the intracellular tyrosine kinase domain, several researchers have speculated that it may function as a decoy receptor and exert a negative effect on cell proliferation. In this study, we performed overexpression experiments with TetOn‑inducible cell clones and downregulation experiments with siRNA oligonucleotides, and found that FGFRL1 had absolutely no effect on cell growth and proliferation. Likewise, we did not observe any influence of FGFRL1 on ERK1/2 activation and on the phosphorylation of 250 other signaling proteins analyzed by the Kinexus antibody microarray. On the other hand, with bacterial petri dishes, we observed a clear effect of FGFRL1 on cell adhesion during the initial hours after cell seeding. Our results suggest that FGFRL1 is a cell adhesion protein similar to the nectins rather than a signaling receptor similar to FGFR1-FGFR4.

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