The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells

Huang,Hsiu-Chin; Lin,Hsuan; Huang,Min-Chuan

doi:10.3892/ijmm.2017.2884

The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells

Authors:
- Hsiu-Chin Huang
- Hsuan Lin
- Min-Chuan Huang
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Affiliations: Renorigin Innovation Institute, Taipei 11560, Taiwan, R.O.C., Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei 100, Taiwan, R.O.C.
Published online on: February 9, 2017 https://doi.org/10.3892/ijmm.2017.2884
Pages: 595-602
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immunomodulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five LfcinB-related peptides was examined. We found that LfB17‑34, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB17‑34 increased in vitro tyrosinase activity and melanin content in a dose-dependent manner. The results of RT-qPCR and western blot analyses showed that LfB17‑34 increased the mRNA and protein expression of tyrosinase and tyrosinase-related protein 1 (Trp1). Moreover, LfB17‑34 inhibited the phosphorylation of MAPK/Erk, but not p38 and Akt, and constitutively active MEK was able to reverse the LfB17-34-enhanced pigmentation, melanin content, and tyrosinase activity, suggesting a role of Erk signaling in the process of LfB17‑34-mediated pigmentation. Taken together, these results suggest that LfB17‑34 induces melanogenesis in B16F10 cells primarily through increased tyrosinase expression and activity and that LfB17‑34 could be further developed for the treatment of hypopigmentation disorders.

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1	Tobin DJ: The cell biology of human hair follicle pigmentation. Pigment Cell Melanoma Res. 24:75–88. 2011. View Article : Google Scholar
2	Dessinioti C, Stratigos AJ, Rigopoulos D and Katsambas AD: A review of genetic disorders of hypopigmentation: Lessons learned from the biology of melanocytes. Exp Dermatol. 18:741–749. 2009. View Article : Google Scholar : PubMed/NCBI
3	Panhard S, Lozano I and Loussouarn G: Greying of the human hair: A worldwide survey, revisiting the '50' rule of thumb. Br J Dermatol. 167:865–873. 2012. View Article : Google Scholar : PubMed/NCBI
4	Ezzedine K, Eleftheriadou V, Whitton M and van Geel N: Vitiligo. Lancet. 386:74–84. 2015. View Article : Google Scholar : PubMed/NCBI
5	Falabella R and Barona MI: Update on skin repigmentation therapies in vitiligo. Pigment Cell Melanoma Res. 22:42–65. 2009. View Article : Google Scholar
6	Hsiao JJ and Fisher DE: The roles of microphthalmia-associated transcription factor and pigmentation in melanoma. Arch Biochem Biophys. 563:28–34. 2014. View Article : Google Scholar : PubMed/NCBI
7	Gifford JL, Hunter HN and Vogel HJ: Lactoferricin: A lactoferrin-derived peptide with antimicrobial, antiviral, antitumor and immunological properties. Cell Mol Life Sci. 62:2588–2598. 2005. View Article : Google Scholar : PubMed/NCBI
8	Centeno JM, Burguete MC, Castelló-Ruiz M, Enrique M, Vallés S, Salom JB, Torregrosa G, Marcos JF, Alborch E and Manzanares P: Lactoferricin-related peptides with inhibitory effects on ACE-dependent vasoconstriction. J Agric Food Chem. 54:5323–5329. 2006. View Article : Google Scholar : PubMed/NCBI
9	Fernández-Musoles R, López-Díez JJ, Torregrosa G, Vallés S, Alborch E, Manzanares P and Salom JB: Lactoferricin B-derived peptides with inhibitory effects on ECE-dependent vasoconstriction. Peptides. 31:1926–1933. 2010. View Article : Google Scholar : PubMed/NCBI
10	Ruiz-Giménez P, Ibáñez A, Salom JB, Marcos JF, López-Díez JJ, Vallés S, Torregrosa G, Alborch E and Manzanares P: Antihypertensive properties of lactoferricin B-derived peptides. J Agric Food Chem. 58:6721–6727. 2010. View Article : Google Scholar : PubMed/NCBI
11	Yan D, Chen D, Shen J, Xiao G, van Wijnen AJ and Im HJ: Bovine lactoferricin is anti-inflammatory and anti-catabolic in human articular cartilage and synovium. J Cell Physiol. 228:447–456. 2013. View Article : Google Scholar
12	Nakajima M, Shinoda I, Samejima Y, Miyauchi H, Fukuwatari Y and Hayasawa H: A simple and quantitative cell-blotting assay for evaluation of pigmentation of cultured melanocytes and its use in demonstrating the depigmenting effect of lactoferrin. Arch Dermatol Res. 289:180–183. 1997. View Article : Google Scholar : PubMed/NCBI
13	Hsu WM, Che MI, Liao YF, Chang HH, Chen CH, Huang YM, Jeng YM, Huang J, Quon MJ, Lee H, et al: B4GALNT3 expression predicts a favorable prognosis and suppresses cell migration and invasion via β₁ integrin signaling in neuroblastoma. Am J Pathol. 179:1394–1404. 2011. View Article : Google Scholar : PubMed/NCBI
14	Lee J, Jung E, Park J, Jung K, Park E, Kim J, Hong S, Park J, Park S, Lee S, et al: Glycyrrhizin induces melanogenesis by elevating a cAMP level in b16 melanoma cells. J Invest Dermatol. 124:405–411. 2005. View Article : Google Scholar : PubMed/NCBI
15	Bellei B, Maresca V, Flori E, Pitisci A, Larue L and Picardo M: p38 regulates pigmentation via proteasomal degradation of tyrosinase. J Biol Chem. 285:7288–7299. 2010. View Article : Google Scholar : PubMed/NCBI
16	Wang D, Xu X, Ma H, Yue X, Li C and Zhu W: Optimization of the method for the culture of melanocyte precursors from hair follicles and their activation by 1,25-dihydroxyvitamin D3. Exp Ther Med. 6:967–972. 2013.PubMed/NCBI
17	Che MI, Huang J, Hung JS, Lin YC, Huang MJ, Lai HS, Hsu WM, Liang JT and Huang MC: β1, 4-N-acetylgalactosaminyltrans ferase III modulates cancer stemness through EGFR signaling pathway in colon cancer cells. Oncotarget. 5:3673–3684. 2014. View Article : Google Scholar : PubMed/NCBI
18	Wu M, Hemesath TJ, Takemoto CM, Horstmann MA, Wells AG, Price ER, Fisher DZ and Fisher DE: c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi. Genes Dev. 14:301–312. 2000.PubMed/NCBI
19	Bae JS, Han M, Yao C and Chung JH: Chaetocin inhibits IBMX-induced melanogenesis in B16F10 mouse melanoma cells through activation of ERK. Chem Biol Interact. 245:66–71. 2016. View Article : Google Scholar : PubMed/NCBI
20	Son YO, Lee SA, Kim SS, Jang YS, Chun JC and Lee JC: Acteoside inhibits melanogenesis in B16F10 cells through ERK activation and tyrosinase down-regulation. J Pharm Pharmacol. 63:1309–1319. 2011. View Article : Google Scholar : PubMed/NCBI