Vitamin D attenuates hyperoxia-induced lung injury through downregulation of Toll-like receptor 4

Yao,Li; Shi,Yongyan; Zhao,Xinyi; Hou,Ana; Xing,Yujiao; Fu,Jianhua; Xue,Xindong

doi:10.3892/ijmm.2017.2961

Vitamin D attenuates hyperoxia-induced lung injury through downregulation of Toll-like receptor 4

Authors:
- Li Yao
- Yongyan Shi
- Xinyi Zhao
- Ana Hou
- Yujiao Xing
- Jianhua Fu
- Xindong Xue
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Affiliations: Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
Published online on: April 21, 2017 https://doi.org/10.3892/ijmm.2017.2961
Pages: 1403-1408
Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

With considerable morbidity and mortality, bronchopulmonary dysplasia (BPD) is a focus of attention in neonatology. Hyperoxia-induced lung injury has long been used as a model of BPD. Among all the signaling pathways involved, Toll-like receptor 4 (TLR4) has been demonstrated to play an important role, and is known to be regulated by vitamin D. This study aimed at elucidating the effect of vitamin D on hyperoxia-induced lung injury and the role of TLR4 in the process. Vitamin D was administered to hyperoxia-treated neonatal rats to investigate changes in the morphology of lungs and expression of pro-inflammatory cytokines, apoptotic proteins and TLR4. Vitamin D attenuated hyperoxia-induced lung injury by protecting the integrity of the lung structure, decreasing extracellular matrix deposition and inhibiting inflammation. The upregulation of TLR4 by hyperoxia was ameliorated by vitamin D and apoptosis was reduced. Vitamin D administration antagonized the activation of TLR4 and therefore alleviated inflammation, reduced apoptosis and preserved lung structure.

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