Open Access

Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans

  • Authors:
    • Ai-Xiu Gong
    • Jing-Han Zhang
    • Jing Li
    • Jun Wu
    • Lin Wang
    • Deng-Shun Miao
  • View Affiliations

  • Published online on: July 12, 2017     https://doi.org/10.3892/ijmm.2017.3065
  • Pages: 647-660
  • Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

There are anatomical and functional differences between human dental pulp (DP) and periodontal ligament (PDL). However, the molecular biological differences and function of these tissues are poorly understood. In the present study, we employed a cDNA microarray array to screen for differentially expressed genes (DEGs) between human DP and PDL tissues, and used the online software WebGestalt to perform the functional analysis of the DEGs. In addition, the STRING database and KEGG pathway analysis were applied for interaction network and pathway analysis of the DEGs. DP and PDL samples were obtained from permanent premolars (n=16) extracted for orthodontic purposes. The results of the microarray assay were confirmed by RT-qPCR. The DEGs were found to be significantly associated with the extracellular matrix and focal adhesion. A total of 10 genes were selected to confirm the results. The mRNA levels of integrin alpha 4 (ITGA4), integrin alpha 8 (ITGA8), neurexin 1 (NRXN1) and contactin 1 (CNTN1) were significantly higher in the DP than in the PDL tissues. However, the levels of collagen type XI alpha 1 (COL11A1), aggrecan (ACAN), collagen type VI alpha 1 (COL6A1), chondroadherin (CHAD), laminin gamma 2 (LAMC2) and laminin alpha 3 (LAMA3) were higher in the PDL than in the DP samples. The gene expression profiles provide novel insight into the characterization of DP and PDL tissues, and contribute to our understanding of the potential molecular mechanisms of dental tissue mineralization and regeneration.
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September-2017
Volume 40 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gong A, Zhang J, Li J, Wu J, Wang L and Miao D: Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. Int J Mol Med 40: 647-660, 2017.
APA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., & Miao, D. (2017). Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans. International Journal of Molecular Medicine, 40, 647-660. https://doi.org/10.3892/ijmm.2017.3065
MLA
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40.3 (2017): 647-660.
Chicago
Gong, A., Zhang, J., Li, J., Wu, J., Wang, L., Miao, D."Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans". International Journal of Molecular Medicine 40, no. 3 (2017): 647-660. https://doi.org/10.3892/ijmm.2017.3065