Functional roles of C/EBPα and SUMO‑modification in lung development

Chen,Yuan-Dong; Liu,Jiang-Yan; Lu,Yan-Min; Zhu,Hai-Tao; Tang,Wei; Wang,Qiu-Xia; Lu,Hong-Yan

doi:10.3892/ijmm.2017.3111

Functional roles of C/EBPα and SUMO‑modification in lung development

Authors:
- Yuan-Dong Chen
- Jiang-Yan Liu
- Yan-Min Lu
- Hai-Tao Zhu
- Wei Tang
- Qiu-Xia Wang
- Hong-Yan Lu
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Affiliations: Dean's Office, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Department of Pediatrics, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
Published online on: August 29, 2017 https://doi.org/10.3892/ijmm.2017.3111
Pages: 1037-1046
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

CCAAT enhancer binding protein alpha (C/EBPα) is a transcription factor regulating the core aspects of cell growth and differentiation. The present study investigated the level and functional role of C/EBPα during the development of the rat lung. C/EBPα protein exhibits a dynamic expression pattern. The correlation between the expression of C/EBPα protein and the content of glycogen during lung maturation was analyzed to understand the function of C/EBPα in lung differentiation. The high expression of C/EBPα coincides with the reduction of glycogen in the fetal lung. In addition, the authors identified that changes in the level of C/EBPα are associated with the secretion of pulmonary surfactant. C/EBPα is modified by small ubiquitin-related modifier (SUMO) post-translationally. The results of double immunofluorescence staining and immunoprecipitation demonstrated that SUMO-modified C/EBPα was present in the lung. The sumoylated C/EBPα gradually decreased during lung differentiation and was negatively correlated with pulmonary surfactant secretion, thereby suggesting that the SUMO modification may participate in C/EBPα-mediated lung growth and differentiation. These results indicated that C/EBPα played a role in lung development and provided the insight into the mechanism underlying SUMO-modification.

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