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Vaspin protects against LPS‑induced ARDS by inhibiting inflammation, apoptosis and reactive oxygen species generation in pulmonary endothelial cells via the Akt/GSK‑3β pathway
- Authors:
- Published online on: October 9, 2017 https://doi.org/10.3892/ijmm.2017.3176
- Pages: 1803-1817
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Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Acute respiratory distress syndrome (ARDS) is a severe medical condition, which is associated with a high mortality rate, and is characterized by noncardiogenic pulmonary edema and hypoxemia (1,2). Although ARDS is not a classified pulmonary vascular disease, disruptions in endothelial barrier integrity and dysfunction of endothelial barrier permeability are considered the pathological hallmarks of ARDS, particularly at the very early stage of ARDS development (3,4).
It is well known that the development of ARDS is associated with various predisposing risk factors, among which obesity has been identified in recent epidemiological studies (5–8). Obesity, particularly visceral obesity, has been reported to impair systemic vasculature and is involved in the initiation and progression of cardiovascular diseases (9,10). Adipose tissue is considered a significant endocrine organ that is capable of crosstalk with peripheral organs via diverse bioactive molecules known as adipokines, which participate in the progression of diverse vascular disorders (11–13). Although different from the well-documented effects of obesity on cardiovascular disease, the relationship between obesity and ARDS has proven to be considerably complex; clinical and experimental data have focused on pertinent physiological alterations in obesity and have highlighted the pivotal role of pulmonary vascular 'priming' and neutrophil functional impairment at baseline (8,14). Since persistent, low-grade inflammation as a result of fat accumulation impairs systemic blood vessels and contributes to the development of various obesity-associated vascular diseases, emerging evidence has suggested that obesity alters ARDS pathogenesis by 'priming' the pulmonary endothelial barrier for insult and injury, as well as amplifying the early inflammatory response, thus lowering the threshold required to initiate ARDS via dysregulated adipokine production in obesity. Previous studies have demonstrated that circulating adipokine levels are associated with the initiation and progression of ARDS (15–18).
Visceral adipose tissue-derived serine proteinase inhibitor (vaspin) is identified as a favorable adipokine that is secreted from visceral white adipose tissues, which is associated with glucose tolerance and chronic inflammation (19,20). Vaspin has been reported to counteract the pathogenesis of various obesity-related vascular complications via its anti-inflammatory and anti-apoptotic properties. In a human study, serum vaspin levels were demonstrated to be lower in patients with coronary artery disease (CAD) compared with in the control individuals; this tendency was confirmed in control individuals with higher systolic blood pressure compared with in control individuals with normal blood pressure, thus indicating that vaspin may be a predictor of CAD (21). Furthermore, a previous study reported that lower fasting vaspin levels were correlated with ischemic vascular events in the last 3 months in patients with carotid stenosis compared to those with asymptomatic stenosis (22). Decreased serum vaspin levels were also observed in patients with ankylosing spondylitis and were associated with flow-mediated dilation levels, thus indicating that vaspin may be a marker for detecting early stage atherosclerosis in patients with ankylosing spondylitis (23). Vaspin is believed to serve a local and endocrine role in the initiation and development of vascular disorders by affecting endothelial cells (ECs), thus disrupting vascular homeostasis. A previous study suggested that vaspin, as a ligand for the cell surface 78 kDa glucose-regulated protein (GRP78)/voltage-dependent anion channel complex in ECs, exerted beneficial effects on diabetic vascular complications by promoting proliferation and inhibiting apoptosis under the diabetic milieu via a protein kinase B (Akt)-dependent mechanism (24). It has also been demonstrated that vaspin may significantly attenuate methylglyoxal-induced cell death and reactive oxygen species (ROS) generation in human umbilical vein ECs (HUVECs) (25). Treatment with vaspin also significantly decreased tumor necrosis factor (TNF)-α-induced activation of nuclear factor (NF)-κB, as well as the expression of the adhesion molecules intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin and monocyte chemotactic protein-1 (MCP-1) in human aortic ECs (26). Furthermore, vaspin is capable of preventing apoptosis induced by free fatty acids through upregulation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway in vascular ECs, suggesting the beneficial effects of vaspin on obesity-associated vascular diseases (27). A recent study also indicated that vaspin was able to inhibit the progression of atherosclerotic plaques in apolipoprotein E−/− mice by suppressing endoplasmic reticulum stress-induced macrophage apoptosis (28).
Collectively, these data suggested that vaspin, as a pleiotropic adipokine capable of exerting anti-inflammatory, anti-apoptotic and antioxidant effects on ECs, may exert a favorable role against the progression of obesity-associated vascular complications. Although the pathogenesis of ARDS is considerably complex, hyperpermeability of the pulmonary EC barrier is a pathological hallmark of ARDS at the very early stage, in which obesity, inflammation, apoptosis and ROS serve important roles. Therefore, it may be hypothesized that vaspin contributes to the protection of ARDS via its endothelial-protective effects. Nevertheless, to the best of our knowledge, no previous studies have assessed the effects of vaspin on pulmonary ECs, specifically in the setting of ARDS.
Therefore, the present study aimed to investigate the effects of vaspin on lipopolysaccharide (LPS)-induced ARDS in vivo and in vitro, and to further explore the molecular basis and potential mechanisms underlying these effects. This study aimed to provide a novel insight into the crosstalk between vaspin and ARDS, particularly focusing on the pulmonary EC barrier.
Materials and methods
Chemicals and reagents
LPS (Escherichia coli LPS serotype 0111:B4), sodium pentobarbital, Evans blue dye and dihydroethidium (DHE), goat serum (G9023) and bovine serum albumin (A2153) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Recombinant human vaspin (rh-vaspin) protein was purchased from GeneTex, Inc. (Irvine, CA, USA). The following primary antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA): Anti-ICAM-1 (cat. no. 4915), anti-NF-κB Rel (cat. no. 8242), anti-phosphorylated (p)-NF-κB Rel (Ser536; E1Z1T; cat. no. 13346), anti-Akt (cat. no. 4685) and anti-p-Akt (Ser473; cat. no. 4060), anti-rabbit IgG, HRP-linked antibody (cat. no. 7074) and Alexa Fluor 488-labeled anti-rabbit secondary antibodies (cat. no. 4412). Anti-vascular endothelial (VE)-cadherin (ab205336) and anti-NADPH oxidase antibodies (ab133303) were purchased from Abcam (Cambridge, UK). Anti-GAPDH (cat. no. AP0063), anti-β-catenin (cat. no. BS6879), anti-glycogen synthase kinase (GSK)-3β (cat. no. BS1402) and anti-p-GSK3β (Ser9; cat. no. BS4084P) antibodies were purchased from Bioworld Technology (Nanjing, China). Adenoviral vectors (Ad) expressing β-galactosidase (Ad-β-gal) and full-length vaspin (Ad-vaspin) were constructed by Shanghai GeneChem Co., Ltd. (Shanghai, China) (primer sequences available upon request). Ad-β-gal was used as a control.
LPS-induced ARDS model in mice
C57BL/6 mice (weighing 20–25 g; 10 weeks old; male; of specific-pathogen-free grade; Department of Laboratory Animal Center, Chongqing Medical University, Chongqing, China) were housed under light-controlled conditions (12-h light/dark cycle) at room temperature (25°C) with 60% humidity and were granted ad libitum access to food and water. All animal experimental protocols were implemented in accordance with the instructions of the National Institutes of Health Guide for the Care and Use of Laboratory Animals (29). The present study was approved by the Ethics Committee of the Second Affiliated Hospital of Chongqing Medical University (Chongqing, China). Mice were randomly assigned to 4 groups as follows: the control group not pre-treated with Ad-vaspin (n=24), the control group pre-treated with Ad-vaspin (n=24), the ARDS group not pre-treated with Ad-vaspin and the ARDS group treated with Ad-vaspin (n=24). Mice were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) prior to exposure of the trachea and right internal jugular vein (IJV). Subsequently, 3×107 PFU Ad-vaspin or Ad-β-gal per mouse was injected into the IJV for 3 days prior to LPS or vehicle (PBS) intratracheal instillation. Mice were anesthetized with sodium pentobarbital (50 mg/kg, i.p.). As described previously (18), to establish a mouse model of ARDS, mice were intratracheally instilled with 5 mg/kg LPS (E. coli LPS serotype 0111:B4) in 50 µl sterile PBS, or PBS alone (control group) using an 18-G catheter. The mice were sacrificed 4 h after the LPS injection and lung tissues were harvested and stored at −80°C until further analysis.
Lung histological evaluation
Left lung lobes were harvested, fixed in 3.7% paraformaldehyde (24 h at room temperature), embedded in paraffin wax and cut into 5-µm sections. Subsequently, the sections were stained with hematoxylin and eosin. Histological lung injury in each mouse was evaluated in 5 random fields (×200 and ×400 magnification) using an inverted microscope (TE2000-U; Nikon Corporation, Tokyo, Japan). A standardized scoring system, published by the American Thoracic Society (30), was used to assess histological lung injury in mice.
ELISA
Lung homogenates were used to determine the levels of TNF-α (MTA00B), interleukin (IL)-6 (M6000B) and IL-10 (M1000B) using commercially available ELISA kits (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's protocols. In cultured cells, basal levels of secretion were determined by measuring TNF-α, IL-6 and IL-10 concentration in cell lysates. The effectiveness of the adenoviral vector expression system was confirmed by measuring mean plasma vaspin concentrations in the treated mice using a commercial ELISA kit (DSA120; R&D Systems, Inc.).
Immunohistochemistry
Mouse left lung tissue sections from each group were deparaffinized with xylene, rehydrated in gradient ethanol and incubated in 3% H2O2 at 37°C for 15 min. Subsequently, the sections were rinsed three times in PBS (10 min/wash). Antigen retrieval was performed by immersing the slices in citrate buffer in a microwave at 96°C for 30 min. Tissues were blocked with goat serum albumin in an incubator at room temperature for 1 h and the sections were then incubated with anti-ICAM-1 primary antibodies (1:50 dilution) at 4°C overnight. Sections were washed a further three times with PBS (10 min/wash) and were incubated with a biotin-labeled secondary antibody (1:1,000; ZB-2010; ZSBIO Biotech Co., Ltd., Beijing, China) at 37°C for 30 min, and were then stained with DAB. Sections were counterstained with hematoxylin, dehydrated in gradient ethanol, vitrified with xylene and sealed with neutral resins. Images were captured using an inverted microscope (TE2000-U; Nikon Corporation).
Measurement of Evans blue-dyed albumin (EBDA) concentrations in lung tissue
Pulmonary capillary permeability was assessed by determining EBDA concentrations. The right IJV of mice was injected with EBDA (30 mg/kg). Then mice were sacrificed and lungs free of blood were excised, weighed and homogenized in 1 ml PBS, after which they were extracted in 2 ml formamide (24 h, 60°C) and centrifuged at 5,000 x g for 30 min at 20°C. The absorbance of the supernatants was measured by spectrophotometry at 620 and 740 nm, plotted against a standard curve, normalized, and converted to µg EBDA/g lung tissue.
Analysis of BALF
A trimmed 18-G catheter was inserted into the trachea. A syringe was connected to the catheter, and 1 ml of sterile normal saline was infused into the airway. Four hours after LPS administration, BALF was collected by the intra-tracheal instillation of 1 ml of sterile normal saline followed by repeated aspiration 3 times and centrifugation at 500 x g for 10 min at 4°C. According to manufacturer's instructions, the protein concentrations in the BALF supernatants were determined using a bicinchoninic acid protein assay (BCA) kit (KeyGen Biotech Co., Ltd., Nanjing, China).
Wet/dry (W/D) lung weight ratio
The right upper lung lobes were harvested and weighed to determine wet lung weight. Subsequently, tissues were dried in an oven at 80°C for 24 h and were weighed again to calculate the W/D ratios.
EC culture
Human pulmonary microvascular ECs (HPMECs) were cultured in EC medium (both ScienCell Research Laboratories, Inc., San Diego, CA, USA) supplemented with 10% fetal bovine serum (FBS; cat. no. 0025), 1% endothelial cell growth supplement (ECGS; cat. no. 1052) and 1% penicillin/streptomycin (P/S; cat. no. 0503) (all from ScienCell Research Laboratories, Inc.) in a 5% CO2 incubator at 37°C. Cells between passages 4 and 10 were grown as a monolayer and starved (1% serum) for 6 h prior to each treatment. Human serum concentrations of vaspin are reported to range between 0.1 and 100 ng/ml; therefore, 10 ng/ml vaspin was used in the present study. Cells were pretreated with rh-vaspin (10 ng/ml) or PBS as a control for 24 h, after which they were washed with PBS and exposed to LPS or vehicle (PBS) at 100 ng/ml for 2 h. Cell lysates were collected for subsequent analysis at the indicated time intervals.
EC monolayer permeability assay
Permeability was determined based on the paracellular permeability of 70 kDa fluorescein isothiocyanate (FITC)-dextran into the lower chamber as described previously (18). Briefly, HPMECs were grown on 0.4 µm Transwell inserts at 1×105 cells per well. Following the indicated time interval for each treatment, 0.5 ml FITC-dextran (1 mg/ml) was added to the upper wells, and 1.5 ml medium was added to the bottom chamber. Following 1 h incubation in the dark, 50 µl medium was aspirated and absorbance was measured using a luminometer (BioTek Instruments, Inc., Winooski, VT, USA) at an excitation wavelength of 488 nm and an emission wavelength of 520 nm. The basal FITC-dextran permeability for unstimulated monolayers was set at 100%.
EC viability assay
Cell viability was measured using Cell Counting kit-8 (CCK-8). Cell suspensions of each group were seeded in 96-well plates at 2×104 cells/well and were preincu-bated at 37°C in a humidified atmosphere containing 5% CO2. Subsequently, 10 µl CCK-8 solution was added to each well, and the plates were incubated for 2 h in an incubator. The absorbance of each well was measured using a microplate reader at 450 nm (BioTek Instruments, Inc.) at room temperature (25°C). Cell viability was calculated using the following equation: Viability = (ODtest group − ODblank group) / (ODcontrol group − ODblank group) × 100%; where OD refers to optical density.
TdT-mediated dUTP nick end labeling (TUNEL) staining
According to the manufacturer's protocol, TUNEL staining was conducted using an in situ cell death detection kit (Roche Diagnostics GmbH, Mannheim, Germany) to detect apoptosis of HPMECs, as described previously (18). DAPI (Nanjing KeyGen Biotech Co., Ltd.) was used to stain nuclei. In situ apoptosis detection (Abcam, Cambridge, MA, USA) was used to assess the apoptotic rate of paraffin-embedded lung sections according to the manufacturer's protocols. DAB reacted with the horseradish peroxidase (HRP)-labeled sample to generate a dark brown signal at the site of DNA fragmentation. TUNEL-positive cells were counted in 5 randomly selected fields (×400 magnification) under an inverted microscope (TE2000-U; Nikon Corporation).
Annexin V-FITC/propidium iodide (PI) staining
Following the indicated treatments, cells were collected and resuspended in 500 µl 1X Annexin V binding buffer, after which they were incubated with 5 µl Annexin V-FITC and 5 µl PI for 15 min at room temperature in the dark. Flow cytometry (FCM; BD Biosciences, Franklin Lakes, NJ, USA) was performed to detect apoptotic cells.
Western blot analysis
Radioimmunoprecipitation assay buffer was used to extract total protein, and a Membrane and Cytoplasmic Protein Extraction kit (both Nanjing KeyGen Biotech Co., Ltd.) was used to extract protein from cells and the left lung tissues of mice from each treatment group according to the manufacturer's protocols. Bicinchoninic acid kit was used to measure protein concentration. Equivalent amounts of protein (30 µg) were separated by SDS-PAGE and were electrotransferred to polyvinylidene fluoride membranes. Subsequently, the membranes were blocked with 5% dry milk/bovine serum albumin (BSA) at room temperature (25°C) for 1 h, and were immunoblotted with anti-NF-κB Rel (1:1,000), anti-phospho-NF-κB Rel (Ser536; 1:1,000), anti-β-catenin (1:500), anti-VE-cadherin (1:1,000), anti-NADPH oxidase antibody (1:500), anti-GSK-3β (1:500), anti-phospho-GSK-3β (Ser9; 1:500) and anti-GAPDH (1:8,000) primary antibodies overnight at 4°C, followed by incubation with the corresponding HRP-conjugated secondary antibodies (1:5,000). Protein bands were detected according to an enhanced chemiluminescence (ECL) method (EMD Millipore, Billerica, MA, USA) using a Bio-Rad Gel Imaging system and were analyzed with Quantity One software version 4.4.0 (both Bio-Rad Laboratories, Inc., Hercules, CA, USA). The expression levels were determined by measuring the corresponding band intensities.
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Total RNA was isolated from cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's protocol. The relative RNA levels were quantified using a NanoDrop 2000 spectrophotometer (NanoDrop; Thermo Fisher Scientific, Wilmington, DE, USA). Subsequently, 1 µg RNA was used as a template for the generation of cDNA using a HiScript First Strand cDNA synthesis kit (Vazyme, Piscataway, NJ, USA). The reverse transcription reaction conditions were 25°C for 5 min, 42°C for 15 min, and 85°C for 5 min. Gene expression levels of TNF-α, IL-6, VE-cadherin and β-catenin were detected using a HiScript® II One Step qRT-PCR SYBR®-Green kit (Vazyme) and StepOne Real-Time PCR apparatus (Applied Biosystem; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Polymerase chain reaction conditions were as follows: pre-denaturation at 95°C for 30 sec, 40 cycles of denaturation at 95°C for 5 sec, annealing at 55°C for 30 sec, and polymerization at 72°C for 30 sec. Relative gene expression levels were normalized to GAPDH using CFX manager softwareversion 3.0 (Bio-Rad Laboratories, Inc.) according to the comparative Cq (ΔΔCq) method (31). The primer sequences are presented in Table I.
Immunofluorescence staining
Coverslips (3 from each group) of the HPMECs (1×105 cells per well) were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with PBS containing 5% goat serum and incubated with anti-NF-κB p65 antibodies (1:400) at 4°C overnight. Subsequently, coverslips were incubated with Alexa Fluor 488-labeled secondary antibodies (1:500) for 1 h in the dark. Coverslips were rinsed three times with PBS, and the nuclei were stained with DAPI (Nanjing KeyGen Biotech Co., Ltd.) for 5 min. Images were captured by inverted microscopy (TE2000-U; Nikon Corporation) after washing.
Fluorescent phalloidin for F-actin staining
HPMECs (1×105 cells per well) were seeded on coverslips (3 from each group) and were fixed in 3.7% paraformaldehyde in PBS. The coverslips were then rinsed with PBS, permeabilized with 0.1% Triton X-100 in PBS, preincubated with PBS containing 1% BSA and stained with 200 µl Fluorescent-iFluor™ 594 phalloidin solution (Nanjing KeyGen Biotech Co., Ltd.) for 30 min at room temperature. Finally, coverslips were washed, sealed and images were captured by inverted microscopy (TE2000-U; Nikon Corporation).
Detection of ROS production
Intracellular ROS production was detected using the superoxide indicator dihydroethidium (DHE; D1168; Invitrogen; Thermo Fisher Scientific, Inc.). HPMECs were pretreated with rh-vaspin (10 ng/ml) or PBS as a control for 24 h, and were then exposed to PBS or LPS (100 ng/ml) for 4 h. HPMECs were washed twice with PBS, incubated in fresh culture medium without FBS, and were incubated with DHE (10 µM dissolved in dimethyl sulfoxide) for 15 min at 37°C. Once the DHE probe is oxidized by ROS to 2-hydroxyethidium, it intercalates within DNA, resulting in the fluorescent red staining of cell nuclei. Images of the cells were captured using an inverted fluorescence microscope (TE2000-U; Nikon Corporation) from three different fields of view. Fluorescence intensity was measured using ImageJ software (National Institutes of Health, Bethesda, MD, USA), averages were calculated and were normalized to the control.
Lucigenin assay
Following exposure of HPMECs to PBS or LPS (100 ng/ml) for 4 h in the absence or presence of rh-vaspin (100 ng/ml) for 24 h, total cell lysates were harvested. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) activity was determined using a lucigenin assay. As a chemiluminescent probe, lucigenin is used to indicate the presence of superoxide anion radicals in cells. The reaction was performed in a total volume of 200 µl assay buffer containing 10 µM lucigenin (L-6868; NSC-151912; MedChem Express, Monmouth Junction, NJ, USA), 1 mM NADPH, and 15 µg cell lysates. Following incubation for 30 min at 37°C in the dark, absorbance was measured using a luminometer (BioTek Instruments, Inc.) at an excitation wavelength of 540 nm and an emission wavelength of 605 nm. Samples were well mixed and chemiluminescence was continuously measured for 30 min. Chemiluminescence of relative light units per second (RLU/sec) was obtained every 10 sec and the results were calculated as area under the curve and normalized to the control.
Statistical analysis
Data are presented as the mean ± standard deviation. Results are representative of at least three independent experiments performed in triplicate. Unpaired Student's t-test was performed for comparisons between two independent groups. One-way analysis of variance (ANOVA) followed by the Student-Newman-Keuls post hoc test was performed to compare continuous variables with normal distribution from three or more independent groups, and to detect significant differences between particular groups. Kruskal-Wallis ANOVA followed by Mann-Whitney U test was performed to compare continuous variables with abnormal distribution from three or more independent groups, and to detect significant differences between particular groups. P<0.05 (95% confidence interval) was considered to indicate a statistically significant difference at. All statistical analyses were conducted using GraphPad Prism 5.0 (GraphPad Software, Inc., La Jolla, CA, USA).
Results
Vaspin attenuates LPS-induced lung injury and pulmonary inflammation in vivo and in vitro
In order to explore the effects of vaspin on lung histological injury in an LPS-induced mouse model of ARDS, lung histopathological examination was conducted. Mice were systemically instilled with Ad-vaspin or Ad-β-gal as control (3×107 PFU/mouse for 3 days) and were subjected to intratracheal injection with LPS (5 mg/kg) to establish a mouse model of ARDS or with PBS as a control. Plasma vaspin levels were enhanced to 99.0±7.9 ng/ml on day 3 after Ad-vaspin injection, which could not be detected in the control mice treated with Ad-β-gal (data not shown). Mice that were injected with LPS developed ARDS as early as 4 h after LPS insult, as indicated by histopathological alterations in the lungs, including increased inflammatory cell infiltration, thickened alveolar septum, intra-alveolar and interstitial edema fluid, and patchy areas of hemorrhage (Fig. 1A). These histopathological alterations were significantly attenuated following administration of Ad-vaspin (Fig. 1A and B). Since lung injury in ARDS is associated with an exaggerated inflammatory response, the present study further examined the effects of vaspin on pulmonary inflammation. Consistent with the results of lung injury assessment, expression levels of the proinflammatory cytokines TNF-α (Fig. 1C) and IL-6 (Fig. 1D) were reduced in Ad-vaspin-pretreated mice compared with in Ad-β-gal-pretreated mice 4 h post-LPS insult, whereas expression of the anti-inflammatory cytokine IL-10 (Fig. 1E) was increased. In addition, the expression levels of the adhesion molecule, ICAM-1 (Fig. 1F), were reduced in Ad-vaspin-pretreated mice compared with in Ad-β-gal-pretreated mice 4 h post-LPS insult. These findings suggested that vaspin may exert an anti-inflammatory effect on lung injury in a mouse model of LPS-induced ARDS.
Vaspin has previously been reported to exert anti-inflammatory effects on various types of vascular ECs (24–27). Therefore, it may be hypothesized that vaspin mediates mitigation of inflammation in pulmonary ECs. To assess the anti-inflammatory effects of vaspin in vitro, the mRNA expression levels of inflammatory cytokines (TNF-α and IL-6) and endothelial-specific adhesion markers (VCAM-1 and E-selectin) were analyzed in HPMECs 2 h after LPS insult. Consistent with the LPS-mediated inflammatory response in lung tissue, the expression levels of TNF-α (Fig. 2A), IL-6 (Fig. 2B), VCAM-1 (Fig. 2C) and E-selectin (Fig. 2D) were significantly increased in HPMECs following LPS administration, which were reversed by pretreatment with rh-vaspin. The observed anti-inflammatory effects of vaspin were further confirmed by examining the activation of NF-κB, which is a pivotal inflammatory mediator; the results indicated that the phosphorylation and nuclear translocation of the NF-κB Rel subunit were reduced following treatment of HPMECs with rh-vaspin 2 h after LPS insult (Fig. 2E and F). Taken together, these findings indicated that vaspin may exert a protective role during the early stage of LPS-induced ARDS via the suppression of EC inflammation.
Vaspin restores the pulmonary EC barrier following LPS insult in vivo and in vitro
Pulmonary microvascular hyperpermeability is a common feature of ARDS; therefore, it was assessed in vitro and in vivo in the present study (Fig. 3). To address whether vaspin modulates pulmonary endothelial barrier function in vivo, total bronchoalveolar lavage fluid (BALF) protein concentrations, EBDA extravasation and lung W/D ratios were analyzed in mice with LPS-induced ARDS. Lung histological damage was observed to be associated with pulmonary microvascular hyperpermeability following LPS instillation, as manifested by increases in BALF protein concentrations (Fig. 3A), EBDA extravasation (Fig. 3B) and W/D ratios (Fig. 3C) at 4 h after LPS treatment. The time point selected to evaluate the effects of vaspin on ARDS was based on its coincidence with the histological onset of lung injury in mice. Pretreatment with Ad-vaspin significantly attenuated pulmonary microvascular hyperpermeability in a murine model of ARDS (Fig. 3A–C). In addition, to further confirm the ability of vaspin to mitigate LPS-induced EC hyperpermeability in vitro, HPMECs were cultured in the presence or absence of rh-vaspin (10 ng/ml) for 24 h, and the influx of FITC-dextran was measured. Treatment with a physiological concentration of rh-vaspin prevented LPS-induced increases in the influx of FITC-dextran (Fig. 3E). These data indicated that vaspin may significantly attenuate LPS-challenged pulmonary microvascular hyperpermeability in vivo and EC barrier dysfunction in vitro.
Vaspin has no effect on pulmonary EC adherens junctions (AJs), actin cytoskeleton and EC differentiation following LPS insult in vivo and in vitro
The stabilization of interendothelial AJs and the actin cytoskeleton are essential for a restrictive pulmonary EC barrier; lung endothelium permeability can increase due to alterations in AJs and the endothelial cyto-skeleton. Therefore, to further address the effects of vaspin on vascular homeostasis, the expression levels of VE-cadherin and β-catenin, which are two important AJ proteins of ECs, were evaluated in the lung tissue of mice. Notably, analysis of AJs expression did not detect a difference in the Ad-vaspin-pretreated mice compared with those treated with LPS alone (Fig. 3D). In addition, LPS caused a reduction in the mRNA expression levels of AJs (Fig. 3F and G), as well as cell retraction, F-actin reorganization and stress fiber formation in HPMECs, as determined by phalloidin staining (Fig. 3H). However, consistent with the results of the in vivo experiments, AJs gene expression and distribution of the actin cytoskeleton in vaspin-treated HPMECs were not altered compared with the controls treated with LPS alone (Fig. 3F–H). Collectively, these findings indicated that vaspin may exert beneficial effects on pulmonary microvascular hyperpermeability via restoring EC barrier function; however, these effects may not depend on the ability of vaspin to regulate interendothelial AJs or the endothelial cytoskeleton but may be dependent on other mechanisms associated with endothelial barrier integrity.
Vaspin improves pulmonary EC survival and suppresses pulmonary EC apoptosis following LPS insult in vitro and in vivo
Vaspin has been reported to inhibit the apoptosis of ECs, including human aortic ECs, HUVECs and smooth muscle cells (SMCs). Furthermore, the function of the pulmonary endothelial barrier depends on its integrity (24,25,27). Focusing on the beneficial effects of vaspin on the function and integrity of pulmonary ECs at the cellular level, HPMECs were treated with LPS in the presence or absence of rh-vaspin (10 ng/ml, pretreatment for 24 h). CCK-8, TUNEL staining and FCM analyses were performed to ascertain whether vaspin modulates the survival and apoptosis of pulmonary ECs following exposure to LPS (Fig. 4). In HPMECs, pretreatment with rh-vaspin significantly promoted pulmonary EC survival and suppressed pulmonary EC apoptosis under LPS stimulus compared with those pretreated with PBS, as determined by increased cell viability (Fig. 4B), a reduction in the number of TUNEL-positive cells (Fig. 4A and C) and reduced apoptotic rate, as determined by FCM (Fig. 4A and D). In situ apoptotic detection was further performed to verify the effects of vaspin on apoptosis in lung tissues. Compared with in the control group, Ad-vaspin markedly reduced the number of TUNEL-positive cells in lung tissues post-LPS insult (Fig. 4E). These results suggested that vaspin exerts a protective role on HPMECs, at least partially via its prosurvival and anti-apoptotic properties.
Vaspin inhibits LPS-induced ROS generation and NOX activation following LPS insult in vitro
A previous study demonstrated that ROS and NOX mediated EC apoptosis under various insults. Furthermore, vaspin has been demonstrated to inhibit ROS generation by suppressing NOX activation in ECs, including HUVECs. Therefore, the present study further evaluated the effects of vaspin on intracellular ROS production by measuring the fluorescence intensity of the intracellular fluorescent probe, DHE. Compared with HPMECs exposed to LPS alone, pretreatment with rh-vaspin significantly counteracted LPS-induced ROS production in HPMECs (Fig. 5A and B). Constitutive NOX functions as an oxygen sensor that regulates intracellular superoxide organization. Therefore, to elucidate the upstream mechanisms, the effects of vaspin on NOX were further assessed by measuring NOX activity and expression. The results indicated that LPS induced an increase in NOX activity (Fig. 5C) and expression (Fig. 5D and E); however, this was markedly abrogated by pretreatment of HPMECs with vaspin. These findings indicated that the protective effects of vaspin against LPS insults in ECs may be associated with antioxidative properties.
Vaspin activates the Akt-GSK3β signaling pathway in vivo and in vitro
The PI3K/Akt signaling pathway acts as a compensatory regulator of ARDS through its anti-inflammatory, anti-apoptotic and antioxidant effects in response to numerous growth factors. Therefore, to assess the effects of vaspin on the activation of Akt-related signaling in vivo and in vitro, the phosphorylation of Akt and its downstream target GSK3β were assessed by western blotting. The results indicated that p-Akt and p-GSK3β levels were low under non-stressed conditions but were increased in a mouse model of LPS-induced ARDS; however, the differences between these groups were not statistically significant, thus suggesting an endogenous negative feedback mechanism underlying the effects of LPS on the PI3K/Akt pathway. Notably, administration of vaspin enhanced the phosphorylation of Akt and GSK3β in mouse lungs subjected to LPS (Fig. 6A). At the cellular level, rh-vaspin stimulated the phosphorylation of Akt and GSK3β (Fig. 6B). Collectively, these data suggested that vaspin acts as a stimulatory factor for the Akt-GSK3β signaling pathway, and vaspin-induced protection of pulmonary ECs during ARDS may be mediated, at least partially, by activating the Akt-GSK3β signaling pathway.
Discussion
Pulmonary vascular hyperpermeability has long been considered a principal pathological hallmark of ARDS, which is largely responsible for pulmonary edema formation (3,4,32). Therefore, stability and integrity of the pulmonary endothelial barrier are important in the pathogenesis of ARDS. To the best of our knowledge, the present study is the first to provide evidence to suggest that vaspin protects the pulmonary endothelial barrier during LPS-induced ARDS by limiting inflammation, apoptosis and ROS generation in pulmonary ECs. In a murine model of ARDS, systemic administration of Ad-vaspin attenuated lung injuries by suppressing the pulmonary inflammatory response and restoring pulmonary endothelial barrier functions. In HPMECs, treatment with a physiological concentration of rh-vaspin led to attenuation of the inflammatory response, cell apoptosis and NOX-dependent ROS generation, as well as enhancement of EC survival, with no alterations in cell AJs expression or cytoskeletal rearrangement. A further mechanistic study suggested that the beneficial effects of vaspin are, at least partially, mediated by activation of the Akt-GSK3β signaling pathway. Taken together, these data indicated that vaspin may promote the function and refine stability of the pulmonary endothelial barrier via its anti-inflammatory, anti-apoptotic and antioxidative properties in ECs, thus protecting against LPS-induced endothelial barrier hyperpermeability and disruption in the experimental systems of ARDS. Therapeutic interventions that aim to restore vaspin levels may be favorable for the prevention or treatment of ARDS.
At present, adipose tissue is considered a significant endocrine organ that is capable of crosstalk with peripheral organs through various multifunctional adipokines (11). Compared with the well-elucidated effects of adipokines on cardiovascular disease, the association between adipokines and the pathogenesis of ARDS has only recently begun to be elucidated (12,33). Given the systemic abnormalities associated with obesity and the accompanying metabolic syndrome, the effects of obesity on the pathogenesis of ARDS may be associated with numerous facets of the obese state, including dysregulation of adipokine release, which are known to contribute to the development of obesity-associated vascular disorders. The majority of adipokines are proinflammatory, whereas a small number of anti-inflammatory adipokines exert beneficial effects on obesity-associated complications (13). Vaspin functions as a novel predictor of obesity-associated vascular disorders and endothelial damage in human studies (21,23). Furthermore, it has been demonstrated to be involved in the modulation of diverse biological process, including inflammation, apoptosis and oxidative stress in experimental studies (24–28,34), which are potentially implicated in the pathogenesis of ARDS. As the homologous gene of vaspin, α1-antitrypsin is well known for its function in inhibiting serum serine proteases, including neutrophil elastase, trypsin, thrombin and proteinase-3 (35). In addition, α1-antitrypsin has been reported to exert pleiotropic effects in the airways via its antioxidant, antiprotease, anti-inflammatory and anti-apoptotic properties (36–40). From a clinical perspective, it has previously been indicated that there is no therapeutic advantages to enhancing anti-neutrophil elastase activity in ARDS (41,42). Nevertheless, to the best of our knowledge, no study has assessed the effects of vaspin on pulmonary ECs, particularly in the setting of ARDS. Therefore, the effects of vaspin on pulmonary ECs were explored in the present study.
Inflammation serves a vital role in the initiation and progression of ARDS (43,44), and vaspin has been reported to exert anti-inflammatory effects against various insults (26,34). The present study demonstrated that vaspin suppressed the inflammatory response to LPS in lung tissues and HPMECs, as evidenced by a decrease in the levels of proinflammatory cytokines (IL-6 and TNF-α) and ICAM-1 in lung tissues, and a reduction in the activation of NF-κB, which is a pivotal inflammatory mediator, post-LPS stimulation. Consistent with these findings, previous studies have indicated that vaspin may protect ECs from proinflammatory cytokines-induced inflammation by inhibiting activation of NF-κB and the production of its downstream molecules, including ICAM-1, VCAM-1 and MCP-1 (34). However, EC barrier hyperpermeability in ARDS involves complex interactions between ECs and numerous cell types. Furthermore, vaspin is a pleiotropic adipokine, the effects of which in obesity-associated vascular disease are not exclusive to a specific cell type via a single mechanism. Therefore, it cannot be ruled out that vaspin exerts its potential effects on other cell types, including vascular SMCs (VSMCs), polymorphonuclear leukocytes (PMN) or macrophages, all of which serve important but distinct roles in ARDS progression. Results from previous studies have indicated that vaspin has anti-inflammatory and antimigratory roles in VSMCs, and may prevent the expression of ICAM-1 in cultured VSMCs (45,46). Further investigation is still required to fully elucidate the comprehensive effects of vaspin on ARDS. Taken together, although the present study did not directly detect or rule out the effects of vaspin on numerous cell types in ARDS, the present findings suggested that vaspin, as a multifunctional adipokine, exerts beneficial effects on ARDS via its anti-inflammatory function in lung endothelium.
It is widely accepted that compromised EC integrity and subsequent dysfunction of the pulmonary EC barrier contribute to pulmonary endothelial barrier hyperpermeability, thus leading to protein-rich leakage and PMN infiltration under ARDS conditions (4,32). Therefore, therapeutic approaches that restore pulmonary endothelial barrier integrity and function are of vital importance in the progression of ARDS (3,4). In the present study, aggravation of pulmonary microvascular hyperpermeability in a mouse model of ARDS was significantly suppressed following systemic administration of Ad-vaspin, thus suggesting that vaspin promotes endothelial barrier function in vivo, as evidenced by a significant reduction in capillary leakage (measured by BALF protein concentrations, EBDA extravasation and W/D ratios). Further in vitro investigations supported this beneficial property at the cellular level; the in vitro results demonstrated that vaspin reversed LPS-induced increases in the influx of FITC-dextran in HPMECs. The EC barrier consists of the cytoskeleton, AJs and tight junctions. Stabilization of endothelial AJs and the actin cytoskeleton is essential for a restrictive pulmonary EC barrier (47,48). Notably, in the present study, no difference in AJ protein expression and cytoskeletal organization was detected in HPMECs treated with LPS. Therefore, it may be hypothesized that vaspin-induced promotion of EC barrier function is mediated by other mechanisms associated with cell integrity, rather than regulation of cell structure.
It has previously been hypothesized that a reduction in pulmonary endothelial cell apoptosis represents an important therapeutic target for ARDS (49). Vaspin has been reported to ameliorate vascular injuries under the diabetic milieu, thus exerting beneficial effects on diabetic vascular complications by promoting the proliferation and inhibiting the apoptosis of ECs (24). In addition, vaspin has been demonstrated to attenuate methylglyoxal-induced cell death in HUVECs (25). To confirm whether vaspin-induced suppression of pulmonary hyperpermeability in response to LPS is due to its protection of pulmonary ECs, the present study examined the direct effects of vaspin on pulmonary endothelium at the cellular level. Vaspin-mediated attenuation of EC barrier dysfunction was associated with a significant decrease in HPMECs apoptosis under LPS stimulation. These findings suggested that vaspin exerts its protective effects on pulmonary EC barrier during the LPS-induced ARDS milieu is dependent, at least partially, on its ability to attenuate apoptosis of ECs, thus reinforcing barrier integrity and restoring EC barrier function.
Endothelial dysfunction can be caused by various insults, among which ROS production is markedly involved in ARDS pathogenesis (50,51). ROS are produced by NOX and function as second messengers of cytokines that are formed in response to various stimuli. It has previously been demonstrated that the primary downstream target of ROS is apoptosis signal-regulating kinase 1, which regulates the balance between cell death and survival (52,53). In the present study, the results demonstrated that vaspin may inhibit ROS production and NOX activation, thus alleviating damage to the pulmonary ECs barrier caused by LPS-induced oxidative burst. Consistent with these findings, vaspin has been reported to exert an antioxidative effect on various types of cells, including ECs (25,46).
The present study also explored the underlying mechanism mediating the favorable effects of vaspin. The Akt-associated pathway has a central role in cell signaling with diverse cellular functions, including cell viability, proliferation, angiogenesis and migration. The Akt signaling pathway acts as an endogenous negative feedback or compensatory mechanism that serves to suppress proinflammatory and apoptotic events in response to injurious stimuli (54). According to the findings of a previous study, vaspin can activate Akt-associated signaling (27); therefore, the present study detected the phosphorylation levels of Akt and its substrate GSK3β; the results confirmed that the beneficial effects mediated by vaspin in ARDS were associated with activation of the Akt/GSK3β signaling pathway. However, it is well known that a high degree of functional versatility and overlap exists among the Akt-associated signaling pathways, and the inflammatory response can be regulated by numerous intercellular signals that overlap with each other to modulate cell biology in an integrated manner. With regards to inflammation-associated signaling pathways, we aim to investigate those that induce activation of NF-κB, including stress-activated protein kinases/c-Jun N-terminal kinases, p38 mitogen-activated protein kinases and extracellular signal-regulated kinases 1/2 pathways, in our subsequent studies. Furthermore, although GRP78 is identified as one of the interacting molecules of vaspin on the surface of aortic ECs, further studies are required to confirm the specific mechanism that mediates the beneficial effects of vaspin on pulmonary ECs, more specifically in the pathogenesis of ARDS.
In conclusion, the present study demonstrated that vaspin protects against LPS-induced ARDS through reversing EC barrier dysfunction via the suppression of inflammation, apoptosis and ROS generation in pulmonary ECs, at least partially via activation of the Akt/GSK3β signaling pathway. These findings provide evidence of a causal link between vaspin and EC barrier dysfunction during ARDS, and suggest a potential therapeutic intervention for patients with ARDS in clinical practice.
Abbreviations:
ARDS |
acute respiratory distress syndrome |
CAD |
coronary artery disease |
IJV |
internal jugular vein |
LPS |
lipopolysaccharide |
Ad-vaspin |
adenoviral vector expressing vaspin |
Ad-β-gal |
adenoviral vector expressing β-galactosidase |
rh-vaspin |
recombinant human vaspin |
ECs |
endothelial cells |
EBDA |
Evans blue dyed-albumin |
W/D |
wet/dry |
HPMECs |
human pulmonary microvascular endothelial cells |
SMCs |
smooth muscle cells |
CCK-8 |
Cell Counting kit-8 |
TUNEL |
TdT-mediated dUTP nick end labeling |
FCM |
flow cytometry |
FITC |
fluorescein isothiocyanate |
PI |
propidium iodide |
ICAM |
intercellular cell adhesion molecule |
VCAM |
vascular cell adhesion molecule |
MCP-1 |
monocyte chemotactic protein-1 |
AJ |
adherens junction |
DHE |
dihydroethidium |
ROS |
reactive oxygen species |
NADPH |
nicotinamide adenine dinucleotide phosphate |
Acknowledgments
The present study was supported by the National Natural Science Foundation of China (grant no. 81670071).
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