Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells

Yao,Xiangping; Yu,Taiyong; Zhao,Chen; Li,Youlei; Peng,Ying; Xi,Fengxue; Yang,Gongshe

doi:10.3892/ijmm.2017.3321

Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells

Authors:
- Xiangping Yao
- Taiyong Yu
- Chen Zhao
- Youlei Li
- Ying Peng
- Fengxue Xi
- Gongshe Yang
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Affiliations: College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi 712100, P.R. China
Published online on: December 13, 2017 https://doi.org/10.3892/ijmm.2017.3321
Pages: 1627-1634

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Abstract

Evodiamine is a botanical alkaloid compound extracted from Tetradium plants. Previous studies have reported that evodiamine (Evo) treatment can reduce food uptake and improve insulin resistance in animals . The skeletal muscle comprises about 40% of the body mass of adults and has a vital role in regulating whole body glucose metabolism and energy metabolism. However, the effect of Evo on skeletal muscle is unclear. The main aim of the present study was to investigate the effect of Evo on the differentiation and proliferation of the mouse C2C12 muscle cell line. The results demonstrated that Evo promoted the expression of myogenic marker genes (Myogenin and muscle myosin heavy chain) and increased myoblast differentiation, potentially via activation of the Wnt/β‑catenin pathway. Furthermore, Evo increased mRNA expression of p21, reduced mRNA expression of Cyclin B, Cyclin D and Cyclin E and reduced the percentage of proliferating cells. Also, phosphorylation of ERK1/2 was decreased by Evo treatment during cell proliferation. In conclusion, these findings indicated that Evo has marked effects on skeletal muscle development.

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