Open Access

miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3

  • Authors:
    • Jun Chen
    • Chao Zhou
    • Junhe Li
    • Xiaojun Xiang
    • Ling Zhang
    • Jun Deng
    • Jianping Xiong
  • View Affiliations

  • Published online on: January 18, 2018     https://doi.org/10.3892/ijmm.2018.3405
  • Pages: 1855-1866
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Drug resistance and disease recurrence are major obstacles to the effective treatment of cancer, including gastric cancer (GC). However, the mechanisms of drug resistance remain to be fully elucidated. The present study investigated the roles of microRNA (miR)‑21‑5p in the doxorubicin (DOX) resistance of GC cells and the underlying mechanisms. miR‑21‑5p expression levels were identified to be inversely correlated with two well‑known tumor suppressor genes, phosphatase and tensin homologue and tissue inhibitor of matrix metalloproteinases 3, and were upregulated in GC cell lines in proportion to their degree of resistance. Suppressing miR‑21‑5p expression partially sensitized SGC7901/DOX cells to DOX, suggesting that knockdown of miR‑21‑5p expression may be used as a therapeutic strategy to improve GC cell resistance. Importantly, increased miR‑21‑5p expression levels at diagnosis were correlated with clinicopathological characteristics including advanced stage and poor prognosis, further implying that a relapse of GC may be a consequence of miR‑21‑5p upregulation, thus providing evidence for the potential utility of miR‑21‑5p antagonism to sensitize GC cells to DOX chemotherapy.
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April-2018
Volume 41 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen J, Zhou C, Li J, Xiang X, Zhang L, Deng J and Xiong J: miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3. Int J Mol Med 41: 1855-1866, 2018.
APA
Chen, J., Zhou, C., Li, J., Xiang, X., Zhang, L., Deng, J., & Xiong, J. (2018). miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3. International Journal of Molecular Medicine, 41, 1855-1866. https://doi.org/10.3892/ijmm.2018.3405
MLA
Chen, J., Zhou, C., Li, J., Xiang, X., Zhang, L., Deng, J., Xiong, J."miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3". International Journal of Molecular Medicine 41.4 (2018): 1855-1866.
Chicago
Chen, J., Zhou, C., Li, J., Xiang, X., Zhang, L., Deng, J., Xiong, J."miR‑21‑5p confers doxorubicin resistance in gastric cancer cells by targeting PTEN and TIMP3". International Journal of Molecular Medicine 41, no. 4 (2018): 1855-1866. https://doi.org/10.3892/ijmm.2018.3405