Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.

Zhao,Daqun; Ding,Xiang; Hou,Yiling; Hou,Wanru; Liu,Lu; Xu,Ting; Yang,Danni

doi:10.3892/ijmm.2018.3450

Structural characterization, immune regulation and antioxidant activity of a new heteropolysaccharide from Cantharellus cibarius Fr.

Authors:
- Daqun Zhao
- Xiang Ding
- Yiling Hou
- Wanru Hou
- Lu Liu
- Ting Xu
- Danni Yang
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Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, Nanchong, Sichuan 637009, P.R. China
Published online on: February 1, 2018 https://doi.org/10.3892/ijmm.2018.3450
Pages: 2744-2754
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A new heteropolysaccharide was extracted and purified from the fruiting bodies of Cantharellus cibarius Fr. The Cantharellus cibarius Fr. polysaccharide (CC-1) had a molecular weight of 61,056 kDa and was mainly formed of the glucose and xylose at ratio of 5:1. Structure identification of CC-1 was analysed by a combined application of total hydrolysis, high performance liquid chromatography (HPLC), methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The experimental results showed that CC-1 had a backbone of 1,4-linked-β-D-glucose which branched at O-6 and the branches were mainly composed of 6→1)-α-D-xylopyranose residue. CC-1 exhibited significant in vitro antioxidant effect and proliferation effect of immune cells. The activity study showed CC-1 has ability to clear the ABTS+ free radical and DPPH- free radical in a certain range of concentration. The proliferation activity of the immune cells showed that the proliferation effect on B cells was very significant (P<0.001) in the concentration of 0.625-80 mg/ml; and the effect of T cell proliferation was also very significant (P<0.001) in the concentration of 5-20 mg/ml. The result of this study introduced Cantharellus cibarius Fr. as a possible valuable source in exhibiting unique immunoregulatory and antioxidant properties.

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1	Brown MW, Kolisko M, Silberman JD and Roger AJ: Aggregative multicellularity evolved independently in the eukaryotic supergroup Rhizaria. Curr Biol. 22:1123–1127. 2012. View Article : Google Scholar : PubMed/NCBI
2	Pawlowski J, Audic S, Adl S, Bass D, Belbahri L, Berney C, Bowser SS, Cepicka I, Decelle J, Dunthorn M, et al CBOL Protist Working Group; CBOL protist working group: Barcoding eukaryotic richness beyond the animal, plant, and fungal kingdoms. PLoS Biol. 10:e10014192012. View Article : Google Scholar
3	Mansour-Benamar M, Savoie JM and Chavant L: Valorization of solid olive mill wastes by cultivation of a local strain of edible mushrooms. C R Biol. 336:407–415. 2013. View Article : Google Scholar : PubMed/NCBI
4	Chang ST: The World Mushroom Industry: Trends and technological development. Int J Med Mushrooms. 8:297–314. 2006. View Article : Google Scholar
5	Pacheco L: Sex differences in mushroom gathering: Men expend more energy to obtain equivalent benefits. Evol Hum Behav. 31:289–297. 2010. View Article : Google Scholar
6	Leonti M: The future is written: Impact of scripts on the cognition, selection, knowledge and transmission of medicinal plant use and its implications for ethnobotany and ethnopharmacology. J Ethnopharmacol. 134:542–555. 2011. View Article : Google Scholar : PubMed/NCBI
7	Hussain H, Krohn K, Schulz B, Draeger S, Nazir M and Saleem M: Two new antimicrobial metabolites from the endophytic fungus, Seimatosporium sp. Nat Prod Commun. 7:293–294. 2012.PubMed/NCBI
8	Yang WT, Shi SH, Jiang YL, Zhao L, Chen HL, Huang KY, Yang GL and Wang CF: Genetic characterization of a densovirus isolated from great tit (Parus major) in China. Infect Genet Evol. 41:107–112. 2016. View Article : Google Scholar : PubMed/NCBI
9	Kievit FM, Florczyk SJ, Leung MC, Wang K, Wu JD, Silber JR, Ellenbogen RG, Lee JS and Zhang M: Proliferation and enrichment of CD133(+) glioblastoma cancer stem cells on 3D chitosan-alginate scaffolds. Biomaterials. 35:9137–9143. 2014. View Article : Google Scholar : PubMed/NCBI
10	Obach RS: Pharmacologically active drug metabolites: Impact on drug discovery and pharmacotherapy. Pharmacol Rev. 65:578–640. 2013. View Article : Google Scholar : PubMed/NCBI
11	Guo H, Yi W, Song JK and Wang PG: Current understanding on biosynthesis of microbial polysaccharides. Curr Top Med Chem. 8:141–151. 2008. View Article : Google Scholar : PubMed/NCBI
12	Ferreira SS, Passos CP, Madureira P, Vilanova M and Coimbra MA: Corrigendum to 'Structure-function relationships of immunostimulatory polysaccharides: A review' [Carbohydr Polym. 132 (2015) 378–396]. Carbohydr Polym. 147:557–558. 2016. View Article : Google Scholar : PubMed/NCBI
13	Yeung MK: Molecular and genetic analyses of Actinomyces spp. Crit Rev Oral Biol Med. 10:120–138. 1999. View Article : Google Scholar
14	Ganeshapillai J, Vinogradov E, Rousseau J, Weese JS and Monteiro MA: Clostridium difficile cell-surface polysaccharides composed of pentaglycosyl and hexaglycosyl phosphate repeating units. Carbohydr Res. 343:703–710. 2008. View Article : Google Scholar : PubMed/NCBI
15	Ebert B, Rautengarten C, Guo X, Xiong G, Stonebloom S, Smith-Moritz AM, Herter T, Chan LJ, Adams PD, Petzold CJ, et al: Identification and characterization of a golgi-localized UDP-xylose transporter family from arabidopsis. Plant Cell. 27:1218–1227. 2015. View Article : Google Scholar : PubMed/NCBI
16	Hadley B, Maggioni A, Ashikov A, Day CJ, Haselhorst T and Tiralongo J: Structure and function of nucleotide sugar transporters: Current progress. Comput Struct Biotechnol J. 10:23–32. 2014. View Article : Google Scholar : PubMed/NCBI
17	Dubois M, Gibbs KA, Hamilton JK, Rebers PA and Smith F: Colorimetric methods for the determination of sugars and related substances. Anal Chem. 28:350–356. 1956. View Article : Google Scholar
18	Wang F, Hou Y, Ding X, Hou W, Song B, Wang T, Li J and Zeng Y: Structure elucidation and antioxidant effect of a polysaccharide from Lactarius camphoratum (Bull.) Fr. Int J Biol Macromol. 62:131–136. 2013. View Article : Google Scholar : PubMed/NCBI
19	Kim YT, Kim EH, Cheong C, Williams DL, Kim CW and Lim ST: Structural characterization of beta-D-(1→3, 1→6)-linked glucans using NMR spectroscopy. Carbohydr Res. 328:331–341. 2000. View Article : Google Scholar : PubMed/NCBI
20	Cao W, Li XQ, Liu L, Wang M, Fan HT, Li C, Lv Z, Wang X and Mei Q: Structural analysis of water-soluble glucans from the root of Angelica sinensis (Oliv.) Diels. Carbohydr Res. 341:1870–1877. 2006. View Article : Google Scholar : PubMed/NCBI
21	Wang Z, Luo D and Liang Z: Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers. Carbohydr Polym. 57:241–247. 2004. View Article : Google Scholar
22	Ding X, Hou Y, Hou W, Zhu Y, Fu L and Zhu H: Structure elucidation and anti-tumor activities of water-soluble oligosaccharides from Lactarius deliciosus (L. ex Fr.) Gray. Pharmacogn Mag. 11:716–723. 2015. View Article : Google Scholar : PubMed/NCBI
23	Ding X, Hou Y, Zhu Y, Wang P, Fu L, Zhu H, Zhang N, Qin H, Qu W, Wang F, et al: Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray. Oncol Rep. 33:3162–3170. 2015. View Article : Google Scholar : PubMed/NCBI
24	Gentili A, Caretti F, D'Ascenzo G, Marchese S, Perret D, Di Corcia D and Rocca LM: Simultaneous determination of water-soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 22:2029–2043. 2008. View Article : Google Scholar : PubMed/NCBI
25	Luo A and Fan Y: In vitro antioxidant of a water-soluble polysaccharide from Dendrobium fimhriatum Hook.var.oculatum Hook. Int J Mol Sci. 12:4068–4079. 2011. View Article : Google Scholar : PubMed/NCBI
26	Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, Tripathi PC, Seal T and Mukherjee B: Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol. 84:131–138. 2003. View Article : Google Scholar : PubMed/NCBI
27	Sudo A, Uenishi K and Endo T: Anionic copolymerization of epoxide with bifunctional aromatic lactone derived from 2-methylresorcinol. J Polym Sci Pol Chem. 46:3447–3451. 2008. View Article : Google Scholar
28	Bradford PA, Petersen PJ, Young M, Jones CH, Tischler M and O'Connell J: Tigecycline MIC testing by broth dilution requires use of fresh medium or addition of the biocatalytic oxygen-reducing reagent oxyrase to standardize the test method. Antimicrob Agents Chemother. 49:3903–3909. 2005. View Article : Google Scholar : PubMed/NCBI
29	Ding X, Hou Y and Hou W: Structure feature and antitumor activity of a novel polysaccharide isolated from Lactarius deliciosus Gray. Carbohydr Polym. 89:397–402. 2012. View Article : Google Scholar : PubMed/NCBI
30	Hou Y, Ding X and Hou W: Composition and antioxidant activity of water-soluble oligosaccharides fro Hericium erinaceus. Mol Med Rep. 11:3794–3799. 2015. View Article : Google Scholar
31	Luo D: Identification of structure and antioxidant activity of a fraction of polysaccharide purified from Dioscorea nipponica. Makino. Carbohydr Polym. 71:544–549. 2008. View Article : Google Scholar
32	Hou Y, Ding X, Hou W, Song B, Wang T, Wang F, Li J, Zeng Y, Zhong J, Xu T, et al: Pharmacological evaluation for anticancer and immune activities of a novel polysaccharide isolated from Boletus speciosus Frost. Mol Med Rep. 9:1337–1344. 2014. View Article : Google Scholar : PubMed/NCBI
33	Villares A, García-Lafuente A, Guillamón E and Mateo-Vivaracho L: Separation and characterization of the structural features of macromolecular carbohydrates from wild edible mushrooms. Bioac Carbohyd Diet Fibr. 2:15–21. 2013. View Article : Google Scholar