Anti-inflammatory effects of Passiflora foetida L. in LPS-stimulated RAW264.7 macrophages

Park,Ji-Won; Kwon,Ok-Kyoung; Ryu,Hyung  Won; Paik,Jin-Hyub; Paryanto,Imam; Yuniato,Prasetyawan; Choi,Sangho; Oh,Sei-Ryang; Ahn,Kyung-Seop

doi:10.3892/ijmm.2018.3559

Anti-inflammatory effects of Passiflora foetida L. in LPS-stimulated RAW264.7 macrophages

Authors:
- Ji-Won Park
- Ok-Kyoung Kwon
- Hyung Won Ryu
- Jin-Hyub Paik
- Imam Paryanto
- Prasetyawan Yuniato
- Sangho Choi
- Sei-Ryang Oh
- Kyung-Seop Ahn
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Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do 28116, Republic of Korea, International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Yuseong-gu, Daejeon 34141, Republic of Korea, Center for Pharmaceutical and Medical Technology, The Agency for the Assessment and Application of Technology (BPPT), Kawasan Puspiptek Serpong, Tangerang, Banten 15314, Indonesia
Published online on: March 12, 2018 https://doi.org/10.3892/ijmm.2018.3559
Pages: 3709-3716

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Abstract

Passiflora foetida L. (Passifloraceae), a perennial climber in general, is used for treating many ailments in conventional medicine. In this study, the anti-inflammatory effect of methanolic extracts of P. foetida L. (PFME) and the involvement of nuclear factor-κB (NF-κB) signalling in the regulation of inflammation were investigated. PFME prevented the production of prostaglandin E2 (PGE2) and the expression of inducible cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced macrophage cells. Additionally, PFME reduced the release of pro-inflammatory cytokines. Moreover, in LPS-induced RAW264.7 cells, the phosphorylation of MAPKs (ERK1/2, p38 and JNK) was suppressed by PFME. Furthermore, PFME inhibited the NF-κB activation induced by LPS, which was associated with nuclear p65 levels with the abrogation of IκBα degradation and subsequent decreases. These results indicated that the PFME inhibited the LPS-induced inflammatory and oxidative responses. Therefore, we propose that the PFME may be therapeutic for treating inflammatory diseases.

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