The differentiation of human MSCs derived from adipose and amniotic tissues into insulin-producing cells, induced by PEI@Fe3O4 nanoparticles-mediated NRSF and SHH silencing

Wang,Rui; Zhang,Dianbao; Zhang,Tao; Zhao,Feng; Lang,Hongxin; Lin,Xuewen; Pang,Xining

doi:10.3892/ijmm.2018.3827

The differentiation of human MSCs derived from adipose and amniotic tissues into insulin-producing cells, induced by PEI@Fe3O4 nanoparticles-mediated NRSF and SHH silencing

Authors:
- Rui Wang
- Dianbao Zhang
- Tao Zhang
- Feng Zhao
- Hongxin Lang
- Xuewen Lin
- Xining Pang
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Affiliations: Key Laboratory of Cell Biology and Medical Cell Biology, Department of Stem Cells and Regenerative Medicine, National Health Commission of China, Ministry of Education of China, China Medical University, Shenyang, Liaoning 110122, P.R. China
Published online on: August 14, 2018 https://doi.org/10.3892/ijmm.2018.3827
Pages: 2831-2838

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Abstract

Type 1 diabetes involves the immunologically mediated destruction of insulin‑producing cells (IPCs) in the pancreatic islet. Mesenchymal stem cells (MSCs) have the ability to differentiate into IPCs and have become the most promising means for diabetes therapy. The present study demonstrated that human adipose‑derived stem cells (hADSCs) and human amniotic MSCs (hAMSCs) are able to differentiate into functional IPCs by knocking down neuronal restrictive silencing factor (NRSF) and Sonic hedgehog (SHH). In the current study, PEI@Fe3O4 nanoparticles (NPs) were used to deliver NRSF small interfering (si)RNA and SHH siRNA to hADSCs and hAMSCs. Following infection with PEI@Fe3O4 NPs containing NRSF siRNA and SHH siRNA, the MSCs were induced to differentiate into IPCs. Four specific genes for islet cells were expressed in the differentiated cells. These cells also produced and released insulin in a glucose‑responsive manner. These findings indicated that hADSCs and hAMSCs may be induced to differentiate into IPCs via PEI@Fe3O4 NP‑mediated NRSF and SHH silencing.

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