Identification of three aquaporin subgroups from Blomia tropicalis by transcriptomics

Zhou,Ying; Li,Ling; Qian,Jun; Jia,Haoyuan; Cui,Yubao

doi:10.3892/ijmm.2018.3877

Identification of three aquaporin subgroups from Blomia tropicalis by transcriptomics

Authors:
- Ying Zhou
- Ling Li
- Jun Qian
- Haoyuan Jia
- Yubao Cui
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Affiliations: Department of Pediatric Laboratory, Wuxi Children's Hospital, Wuxi, Jiangsu 214023, P.R. China, Department of Pediatric Respiratory Medicine, Wuxi Children's Hospital, Wuxi, Jiangsu 214023, P.R. China, Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China
Published online on: September 13, 2018 https://doi.org/10.3892/ijmm.2018.3877
Pages: 3551-3561

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Abstract

Aquaporins (AQPs), or water channel proteins, are highly conserved across species. These transmembrane proteins promote water and solute transport across cell membranes. No AQP‑related proteins have been identified in mites to date. The present study used transcriptomics (RNA‑sequencing) to identify potential AQPs in the mite species Blomia tropicalis. Molecular cloning techniques were then used to obtain the full‑length gene sequences encoding these AQP family members, and bioinformatics analyses were used to categorize them based on similarity to AQPs in other species. This approach led to the identification of 5 putative AQP‑coding sequences, known as BlotAQP1‑5 (GenBank accession numbers: KX655540, KX655541, KX655542, KX655543 and KX655544, respectively), which were indexed into all three subgroups, i.e., AQPs, aquaglyceroporins and superAQPs. To the best of our knowledge, these represent the first known AQPs in any mite species. Further studies are required to investigate their functional roles in water transport and their potential as drug targets.

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