Berberine prevents human nucleus pulposus cells from IL‑1β‑induced extracellular matrix degradation and apoptosis by inhibiting the NF‑κB pathway

Lu,Lin; Hu,Jialang; Wu,Qipeng; An,Ying; Cui,Wei; Wang,Junwen; Ye,Zhewei

doi:10.3892/ijmm.2019.4105

Berberine prevents human nucleus pulposus cells from IL‑1β‑induced extracellular matrix degradation and apoptosis by inhibiting the NF‑κB pathway

Authors:
- Lin Lu
- Jialang Hu
- Qipeng Wu
- Ying An
- Wei Cui
- Junwen Wang
- Zhewei Ye
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Affiliations: Department of Orthopaedics, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430033, P.R. China, Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
Published online on: February 21, 2019 https://doi.org/10.3892/ijmm.2019.4105
Pages: 1679-1686
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intervertebral disc degeneration (IDD) is widely considered to be one of the main causes of lower back pain, which is a chronic progressive disease closely related to inflammation, nucleus pulposus (NP) cell apoptosis and extracellular matrix (ECM) degradation. Berberine (BBR) is an alkaloid compound with an anti‑inflammatory effect and has been reported to exert therapeutic action in several inflammatory diseases, including osteoarthritis. Therefore, it was hypothesized that BBR may have a therapeutic effect on IDD through inhibition of the inflammatory response. The aim of the present study was to evaluate the influence of BBR on IDD in interleukin (IL)‑1β‑treated human NP cells in vitro. The results showed that BBR attenuated the upregulation of ECM‑catabolic factors [matrix metalloproteinase (MMP)‑3, MMP‑13, a disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)‑4 and ADAMTS‑5], and the downregulation of ECM‑anabolic factors (type II collagen and aggrecan) following stimulation of the human NP cells with IL‑1β. Treatment with BBR also protected human NP cells from IL‑1β‑induced apoptosis, as determined by western blotting and ﬂow cytometry. Mechanistically, the IL‑1β‑stimulated degradation of IκBα, and the phosphorylation and translocation of nuclear factor (NF)‑κB p65 were found to be attenuated by BBR, indicating that NF‑κB pathway activation was suppressed by BBR in the IL‑1β‑treated human NP cells. The results of the experiments revealed a therapeutic potential of BBR for the prevention or treatment of IDD.

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