Detection of N6‑methyladenosine modification residues (Review)

Zhu,Wei; Wang,Jing‑Zi; Xu,Zhiqiang; Cao,Mengda; Hu,Qiaoli; Pan,Chen; Guo,Miao; Wei,Ji‑Fu; Yang,Haiwei

doi:10.3892/ijmm.2019.4169

Detection of N6‑methyladenosine modification residues (Review)

Authors:
- Wei Zhu
- Jing‑Zi Wang
- Zhiqiang Xu
- Mengda Cao
- Qiaoli Hu
- Chen Pan
- Miao Guo
- Ji‑Fu Wei
- Haiwei Yang
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Affiliations: Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210000, P.R. China
Published online on: April 18, 2019 https://doi.org/10.3892/ijmm.2019.4169
Pages: 2267-2278
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Among a number of mRNA modifications, N6‑methyladenosine (m6A) modification is the most common type in eukaryotes and nuclear‑replicating viruses. m6A has a significant role in numerous cancer types, including leukemia, brain tumors, liver cancer, breast cancer and lung cancer. Although m6A methyltransferases are essential during RNA modifications, the biological functions of m6A and the underlying mechanisms remain to be fully elucidated, predominantly due to the limited detection methods for m6A. In the present review, the currently available m6A detection methods and the respective scope of their applications are presented to facilitate the further investigation of the roles of m6A in biological process.

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