Partial decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

Zhu,Yaofeng; Liu,Bingbing; Zheng,Xuefeng; Wu,Jiajia; Chen,Si; Chen,Zhi; Chen,Tao; Huang,Ziyun; Lei,Wanlong

doi:10.3892/ijmm.2019.4288

Partial decortication ameliorates dopamine depletion‑induced striatal neuron lesions in rats

Authors:
- Yaofeng Zhu
- Bingbing Liu
- Xuefeng Zheng
- Jiajia Wu
- Si Chen
- Zhi Chen
- Tao Chen
- Ziyun Huang
- Wanlong Lei
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Affiliations: Department of Anatomy, Zhongshan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Anesthesiology, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Periodical Center, The Third Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China, Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong 510006, P.R. China
Published online on: July 25, 2019 https://doi.org/10.3892/ijmm.2019.4288
Pages: 1414-1424
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The balance between glutamate (cortex and thalamus) and dopamine (substantia nigra) inputs on striatal neurons is of vital importance. Dopamine deficiency, which breaks this balance and leads to the domination of cortical glutamatergic inputs, plays an important role in Parkinson's disease (PD). However, the exact impact on striatal neurons has not been fully clarified. Thus, the present study aimed to characterize the influence of corticostriatal glutamatergic inputs on striatal neurons after decortication due to dopamine depletion in rats. 6‑Hydroxydopamine was injected into the right medial forebrain bundle to induce dopamine depletion, and/or ibotenic acid into the primary motor cortex to induce decortication. Subsequently, the grip strength test and Morris water maze task indicated that decortication significantly shortened the hang time and the latency that had been increased in the rats subjected to dopamine depletion. Golgi staining and electron microscopy analysis showed that the total dendritic length and dendritic spine density of the striatal neurons were decreased in the dopamine‑depleted rats, whereas decortication alleviated this damage. Immunohistochemistry analysis demonstrated that decortication decreased the number of caspase‑3‑positive neurons in the dopamine‑depleted rats. Moreover, reverse transcription‑quantitative PCR and western blot analyses showed that decortication offset the upregulation of caspase‑3 at both the protein and mRNA levels in the dopamine‑depleted rats. In conclusion, the present study demonstrated that a relative excess of cortical glutamate inputs had a substantial impact on the pathological processes of striatal neuron lesions in PD.

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