Open Access

MicroRNA‑379‑5p suppresses renal fibrosis by regulating the LIN28/let‑7 axis in diabetic nephropathy

  • Authors:
    • Nan Li
    • Li‑Juan Wang
    • Wei‑Long Xu
    • Su Liu
    • Jiang‑Yi Yu
  • View Affiliations

  • Published online on: August 30, 2019     https://doi.org/10.3892/ijmm.2019.4325
  • Pages: 1619-1628
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs or miRs) play an important role in pathological processes in diabetic nephropathy (DN). This study aimed to explore whether miR‑379‑5p is associated with renal fibrosis in DN and to elucidate the underlying mechanisms. In vitro experiments indicated that miR‑379‑5p expression was downregulated by high glucose (HG) treatment in mouse mesangial cells (MMCs). Transfection with miR‑379‑5p mimics suppressed the proliferation and the accumulation of extracellular matrix (ECM) components, which were promoted by HG treatment. LIN28B was proven to be a direct target of miR‑379‑5p by luciferase report assay. In addition, the loss of expression of LIN28B, as well as the decrease in cell proliferation and in the accumulation of ECM components, which were induced by the knockdown of LIN28B, were attenuated in the MMCs following transfection with miR‑379‑5p inhibitors. Furthermore, type 2 diabetic db/db mice were used to examine the efficiency of miR‑379‑5p agomir in the alleviation of renal fibrosis. Consistent with the results of the in vitro experiments, miR‑379‑5p agomir suppressed mesangial cell proliferation and the accumulation of ECM components by regulating the LIN28B/let‑7 pathway. Taken together, the findings of this study suggest that miR‑379‑5p is highly involved in renal fibrosis in DN, and that it may be a potential effective therapeutic target for DN.
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November-2019
Volume 44 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Li N, Wang LJ, Xu WL, Liu S and Yu JY: MicroRNA‑379‑5p suppresses renal fibrosis by regulating the LIN28/let‑7 axis in diabetic nephropathy. Int J Mol Med 44: 1619-1628, 2019.
APA
Li, N., Wang, L., Xu, W., Liu , S., & Yu, J. (2019). MicroRNA‑379‑5p suppresses renal fibrosis by regulating the LIN28/let‑7 axis in diabetic nephropathy. International Journal of Molecular Medicine, 44, 1619-1628. https://doi.org/10.3892/ijmm.2019.4325
MLA
Li, N., Wang, L., Xu, W., Liu , S., Yu, J."MicroRNA‑379‑5p suppresses renal fibrosis by regulating the LIN28/let‑7 axis in diabetic nephropathy". International Journal of Molecular Medicine 44.5 (2019): 1619-1628.
Chicago
Li, N., Wang, L., Xu, W., Liu , S., Yu, J."MicroRNA‑379‑5p suppresses renal fibrosis by regulating the LIN28/let‑7 axis in diabetic nephropathy". International Journal of Molecular Medicine 44, no. 5 (2019): 1619-1628. https://doi.org/10.3892/ijmm.2019.4325