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Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3

  • Authors:
    • Meixia Yang
    • Linxin Lu
    • Zhiqin Kang
    • Tianlong Ma
    • Yu Wang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Shanxi Dayi Hospital, Taiyuan, Shanxi 030032, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1707-1718
    |
    Published online on: September 23, 2019
       https://doi.org/10.3892/ijmm.2019.4349
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Abstract

Unfettered inflammation is a leading cause of multiple organ failures in sepsis. The anti‑inflammatory role of cluster of differentiation (CD)39 has been previously reported. The present study aimed to investigate the role of unfettered inflammation in sepsis‑induced acute kidney injury (AKI). Lipopolysaccharide (LPS) was introduced to construct a sepsis mouse model. Kidney function and pathological changes in mice were measured at 12, 24 and 48 h. CD39 overexpression and inhibition vectors were transfected into renal tubular epithelial (HK‑2) cells, followed by LPS treatment (10 µg/ml), and the cell viability changes at 24 h after treatment were assessed and the expression of NLR family pyrin domain containing 3 (NLRP3), cleaved caspase‑1 and CD39 were determined by performing ELISAs. Cell apoptosis and reactive oxygen species (ROS) levels were determined by flow cytometry. It was found that after LPS administration, kidney injury was the most serious at 24 h in mice. CD39 overexpression could suppress the upregulation of pro‑inflammatory cytokines induced by LPS treatment. In addition, the cell apoptosis and ROS level exhibited an obvious decrease, while cell viability increased. The NLRP3 expression and activity also showed a great inhibition in CD39‑overexpressed cells. By contrast to CD39 overexpression, CD39 inhibition promoted the activation of the NLRP3 inflammasome. These data indicate the protective role of CD39 in LPS‑induced renal tubular epithelial cell damage through inhibiting NLRP3 inflammasome activation and that CD39 might be a potential therapeutic target in sepsis‑induced AKI.
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Copy and paste a formatted citation
Spandidos Publications style
Yang M, Lu L, Kang Z, Ma T and Wang Y: Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3. Int J Mol Med 44: 1707-1718, 2019.
APA
Yang, M., Lu, L., Kang, Z., Ma, T., & Wang, Y. (2019). Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3. International Journal of Molecular Medicine, 44, 1707-1718. https://doi.org/10.3892/ijmm.2019.4349
MLA
Yang, M., Lu, L., Kang, Z., Ma, T., Wang, Y."Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3". International Journal of Molecular Medicine 44.5 (2019): 1707-1718.
Chicago
Yang, M., Lu, L., Kang, Z., Ma, T., Wang, Y."Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3". International Journal of Molecular Medicine 44, no. 5 (2019): 1707-1718. https://doi.org/10.3892/ijmm.2019.4349
Copy and paste a formatted citation
x
Spandidos Publications style
Yang M, Lu L, Kang Z, Ma T and Wang Y: Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3. Int J Mol Med 44: 1707-1718, 2019.
APA
Yang, M., Lu, L., Kang, Z., Ma, T., & Wang, Y. (2019). Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3. International Journal of Molecular Medicine, 44, 1707-1718. https://doi.org/10.3892/ijmm.2019.4349
MLA
Yang, M., Lu, L., Kang, Z., Ma, T., Wang, Y."Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3". International Journal of Molecular Medicine 44.5 (2019): 1707-1718.
Chicago
Yang, M., Lu, L., Kang, Z., Ma, T., Wang, Y."Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3". International Journal of Molecular Medicine 44, no. 5 (2019): 1707-1718. https://doi.org/10.3892/ijmm.2019.4349
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