TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway

Tang,Genxiong; Tang,Qi; Jia,Lizhou; Chen,Yuan; Lin,Liangyuan; Kuai,Xingwang; Gong,Aixiu; Feng,Zhengqing

doi:10.3892/ijmm.2019.4378

TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway

Authors:
- Genxiong Tang
- Qi Tang
- Lizhou Jia
- Yuan Chen
- Liangyuan Lin
- Xingwang Kuai
- Aixiu Gong
- Zhengqing Feng
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Affiliations: Department of Stomatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, National Health Commission Key Laboratory of Antibody Technique, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of Otolaryngology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, P.R. China, Department of Basic Medicine, Jiangsu College of Nursing, Huai'an, Jiangsu 223001, P.R. China, Department of Pathology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China
Published online on: October 21, 2019 https://doi.org/10.3892/ijmm.2019.4378
Pages: 2161-2170
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Most malignant neoplasms of the oral cavity are oral squamous cell carcinoma (OSCC), which is a type of highly malignant tumor with a propensity for forming distant metastases. Trophoblast cell surface antigen 2 (TROP2) is a transmembrane protein that is overexpressed in several types of tumor cells, although its role and regulatory mechanism in OSCC have not been determined. The aim of the present study was to examine the effects of TROP2 in human OSCC cell lines. The present study demonstrated that TROP2 protein expression was upregulated in OSCC cell lines. Transfection of short hairpin RNA (shRNA) targeting TROP2 (sh‑TROP2) reduced cell proliferation, migration and invasion of OSCC cell lines, whereas overexpression of TROP2 increased proliferation, migration and invasion. sh‑TROP2 transfection in OSCC cell lines inhibited tumor growth in OSCC mouse models. Furthermore, TROP2 expression activated the phosphoinositide 3‑kinase (PI3K)/Akt signaling pathway in human OSCC cells. These results suggest that TROP2 induces cell growth, migration and invasion through activation of the PI3K/Akt signaling pathway in OSCC cells.

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