Long non‑coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA‑944 and thereby increasing HOXB5 expression Retraction in /10.3892/ijmm.2022.5183

Lin,Cong; Zou,Yanan; Li,Ruijing; Liu,Daofeng

doi:10.3892/ijmm.2020.4581

Long non‑coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA‑944 and thereby increasing HOXB5 expression

Retraction in: /10.3892/ijmm.2022.5183

Authors:
- Cong Lin
- Yanan Zou
- Ruijing Li
- Daofeng Liu
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Affiliations: Department of Stomatology, Shengli Oilfield Central Hospital, Dongying, Shandong 257034, P.R. China
Published online on: April 16, 2020 https://doi.org/10.3892/ijmm.2020.4581
Pages: 119-130
Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A long non‑coding RNA (lncRNA) called prostate cancer‑associated non‑coding RNA 1 (PRNCR1) serves crucial roles in the aggressive phenotypes of colorectal cancer and non‑small cell lung cancer. However, there is little research on the expression profile, clinical value and detailed functions of PRNCR1 in tongue squamous cell carcinoma (TSCC). The aim of the present study was to determine PRNCR1 expression in TSCC and to examine the involvement of PRNCR1 in TSCC progression. The molecular mechanisms behind the oncogenic effects of PRNCR1 in TSCC cells were also investigated. PRNCR1 was revealed to be upregulated in TSCC tumors and cell lines. The high PRNCR1 expression showed a significant correlation with tumor size, clinical stage, lymph node metastasis, and shorter overall survival times among patients with TSCC. A PRNCR1‑knockdown reduced TSCC cell proliferation, migration and invasion, and increased apoptosis in vitro. Additionally, the PRNCR1‑knockdown slowed down in vivo tumor growth of TSCC cells. With regards to the mechanism, PRNCR1 acted as a competing endogenous RNA on microRNA‑944 (miR‑944) in TSCC cells, and the effects of the PRNCR1‑knockdown were reversed by an miR‑944‑knockdown. HOXB5 was validated as a direct target gene of miR‑944 in TSCC cells, and HOXB5 expression was found to be positively regulated by PRNCR1. Furthermore, resumption of HOXB5 expression reversed the tumor‑suppressive actions of miR‑944 in TSCC cells. In conclusion, PRNCR1 acts as an oncogenic lncRNA in TSCC through the upregulation of HOXB5 by sponging miR‑944, thereby indicating a potential therapeutic target in TSCC.

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