Open Access

Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure

  • Authors:
    • Peng Zhao
    • Yunkai Wang
    • Luping Zhang
    • Jinhua Zhang
    • Ning Liu
    • Hongqiang Wang
  • View Affiliations

  • Published online on: December 30, 2020     https://doi.org/10.3892/ijmm.2020.4838
  • Article Number: 5
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Heart failure (HF) is a serious threat to human health. Long noncoding RNAs (lncRNAs) are critical regulators of HF. The aim of the study was to investigate the molecular mechanism of MALAT1 in HF rats. MALAT1 expression was detected in serum of normal volunteers and HF patients, HF rats and isoproterenol (ISO)‑induced H9C2 cells, and its diagnostic value was evaluated in HF patients. Indexes related to cardiac functions and hemodynamics, myocardial injury, lipid metabolism, lipid oxidation, and inflammation were detected. Moreover, the downstream mechanism of MALAT1 was predicted and verified and in vivo experiments were further performed in ISO‑induced H9C2 cells to verify the effects of MALAT1 in HF. MALAT1 was highly expressed in serum of HF patients, HF rats and ISO‑induced H9C2 cells and was valuable in predicting HF. Inhibition of MALAT1 increased cardiac function and anti‑inflammation and alleviated myocardial injury, lipid metabolism, lipid oxidation and apoptosis rates. Inhibition of MALAT1 reduced H9C2 cell injury. MALAT1 competitively bound to microRNA (miR)‑532‑3p to upregulate LDLR protein. Inhibition of miR‑532‑3p weakened the protective effect of downregulated MALAT1 against H9C2 cell injury. We concluded that MALAT1 upregulated LDLR expression by competitively binding to miR‑532‑3p, thereby increasing pathological injury in HF.
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March-2021
Volume 47 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhao P, Wang Y, Zhang L, Zhang J, Liu N and Wang H: Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure. Int J Mol Med 47: 5, 2021.
APA
Zhao, P., Wang, Y., Zhang, L., Zhang, J., Liu, N., & Wang, H. (2021). Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure. International Journal of Molecular Medicine, 47, 5. https://doi.org/10.3892/ijmm.2020.4838
MLA
Zhao, P., Wang, Y., Zhang, L., Zhang, J., Liu, N., Wang, H."Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure". International Journal of Molecular Medicine 47.3 (2021): 5.
Chicago
Zhao, P., Wang, Y., Zhang, L., Zhang, J., Liu, N., Wang, H."Mechanism of long non‑coding RNA metastasis‑associated lung adenocarcinoma transcript 1 in lipid metabolism and inflammation in heart failure". International Journal of Molecular Medicine 47, no. 3 (2021): 5. https://doi.org/10.3892/ijmm.2020.4838