Structural analysis and macrophage activation of a novel β‑glucan isolated from <em>Cantharellus cibarius</em>

Qu,Yunhe; Zhao,Xiaolin; Guo,Huijun; Meng,Yue; Wang,Yumeng; Zhou,Yifa; Sun,Lin

doi:10.3892/ijmm.2021.4883

Structural analysis and macrophage activation of a novel β‑glucan isolated from Cantharellus cibarius

Authors:
- Yunhe Qu
- Xiaolin Zhao
- Huijun Guo
- Yue Meng
- Yumeng Wang
- Yifa Zhou
- Lin Sun
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Affiliations: Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, P.R. China
Published online on: February 10, 2021 https://doi.org/10.3892/ijmm.2021.4883
Article Number: 50
Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to investigate the structure and immunomodulatory activity of a polysaccharide. A novel acidic β‑glucan (WCCP‑A‑b; molecular weight, 7.3 kDa) was purified from the fruiting bodies of the edible mushroom Cantharellus cibarius, which possesses high nutritional values. WCCP‑A‑b was composed primarily of glucose (89.7%) and glucuronic acid (8.8%). Methylation and nuclear magnetic resonance analysis suggested that WCCP‑A‑b contained β‑D‑1,6‑glucan as its main chain, which was substituted at O‑3 by β‑1,3‑D‑Glcp oligosaccharides or a single‑unit of β‑Glcp residues. Minor β‑1,4‑D‑GlcpA residues may also be present in the side chains. The degree of branching was ~20.9%. Moreover, WCCP‑A‑b possessed a macrophage activating effect by promoting the secretion of nitric oxide, TNF‑α and IL‑6 in a dose‑dependent manner. At a cellular mechanistic level, WCCP‑A‑b activated macrophages via the MAPK signaling pathway. The present results provided useful information for supporting further investigations on the structure‑activity association of polysaccharides from C. cibarius, and indicated that the novel β‑glucan may be a potent natural immunomodulator, thus promoting the application of C. cibarius as a valuable source for functional food.

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