Current and innovative methods for the diagnosis of COVID‑19 infection (Review)
- Authors:
- Luca Falzone
- Giuseppe Gattuso
- Aristidis Tsatsakis
- Demetrios A. Spandidos
- Massimo Libra
-
Affiliations: Epidemiology and Biostatistics Unit, National Cancer Institute‑IRCCS ‘Fondazione G. Pascale’, I‑80131 Naples, Italy, Department of Biomedical and Biotechnological Sciences, University of Catania, I‑95123 Catania, Italy, Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece - Published online on: April 12, 2021 https://doi.org/10.3892/ijmm.2021.4933
- Article Number: 100
-
Copyright: © Falzone et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
This article is mentioned in:
Abstract
Bontempi E: The europe second wave of COVID-19 infection and the Italy 'strange' situation. Environ Res. 193:1104762021. View Article : Google Scholar | |
Cacciapaglia G, Cot C and Sannino F: Second wave COVID-19 pandemics in Europe: A temporal playbook. Sci Rep. 10:155142020. View Article : Google Scholar : PubMed/NCBI | |
COVID-19 Dashboard by the Center for Systems Science and Engineering (CSSE) Jonhs Hopkins University (JHU). https://gisanddata.maps.arcgis.com/apps/opsdashboard/index.html#/bda7594740fd40299423467b48e9ecf6. Accessed February 27, 2021. | |
Polack FP, Thomas SJ, Kitchin N, Absalon J, Gurtman A, Lockhart S, Perez JL, Pérez Marc G, Moreira ED, Zerbini C; C4591001 Clinical Trial Group; et al: Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine. N Engl J Med. 383:2603–2615. 2020. View Article : Google Scholar : PubMed/NCBI | |
European Medicine Agency (EMA): COVID-19 Vaccines. https://www.ema.europa.eu/en/human-regulatory/overview/public-health-threats/coronavirus-disease-covid-19/treatments-vaccines/covid-19-vaccines. Accessed February 27, 2021. | |
Dong E, Du H and Gardner L: An interactive web-based dashboard to track COVID-19 in real time. Lancet Infect Dis. 20:533–534. 2020. View Article : Google Scholar : PubMed/NCBI | |
Pullano G, Di Domenico L, Sabbatini CE, Valdano E, Turbelin C, Debin M, Guerrisi C, Kengne-Kuetche C, Souty C, Hanslik T, et al: Underdetection of cases of COVID-19 in France threatens epidemic control. Nature. 590:134–139. 2021. View Article : Google Scholar | |
O'Dowd A: Covid-19: Avoid indoor mixing over Christmas or risk third wave, warns iSAGE. BMJ. 371:m48322020. View Article : Google Scholar : PubMed/NCBI | |
Farsalinos K, Poulas K, Kouretas D, Vantarakis A, Leotsinidis M, Kouvelas D, Docea AO, Kostoff R, Gerotziafas GT, Antoniou MN, et al: Improved strategies to counter the COVID-19 pandemic: Lockdowns vs. primary and community healthcare. Toxicol Rep. 8:1–9. 2021. View Article : Google Scholar | |
Liu S and Pan C: Differentiating diagnosis of COVID-19 or influenza in patients based on laboratory data during flu season. EClinicalMedicine. 26:1005112020. View Article : Google Scholar : PubMed/NCBI | |
García-Basteiro AL, Chaccour C, Guinovart C, Llupià A, Brew J, Trilla A and Plasencia A: Monitoring the COVID-19 epidemic in the context of widespread local transmission. Lancet Respir Med. 8:440–442. 2020. View Article : Google Scholar : PubMed/NCBI | |
Wu AHB: Screening the General Population for SARS-CoV-2 Virus and COVID-19 Antibodies: A Counterargument. J Appl Lab Med. 5:1107–1110. 2020. View Article : Google Scholar : PubMed/NCBI | |
Rostami A, Sepidarkish M, Leeflang MMG, Riahi SM, Nourollahpour Shiadeh M, Esfandyari S, Mokdad AH, Hotez PJ and Gasser RB: SARS-CoV-2 seroprevalence worldwide: a systematic review and meta-analysis. Clin Microbiol Infect. 27:331–340. 2021. View Article : Google Scholar | |
Wells PM, Doores KJ, Couvreur S, Nunez RM, Seow J, Graham C, Acors S, Kouphou N, Neil SJD, Tedder RS, et al: Estimates of the rate of infection and asymptomatic COVID-19 disease in a population sample from SE England. J Infect. 81:931–936. 2020. View Article : Google Scholar : PubMed/NCBI | |
Ji T, Liu Z, Wang G, Guo X, Akbar Khan S, Lai C, Chen H, Huang S, Xia S, Chen B, et al: Detection of COVID-19: A review of the current literature and future perspectives. Biosens Bioelectron. 166:1124552020. View Article : Google Scholar : PubMed/NCBI | |
Sanyaolu A, Okorie C, Marinkovic A, Ayodele O, Abbasi AF, Prakash S, Ahmed M, Kayode D, Jaferi U and Haider N: Navigating the diagnostics of COVID-19. SN Compr Clin Med. Jul 25–2020.Epub ahead of print. View Article : Google Scholar : PubMed/NCBI | |
European Commission (EC): Current performance of COVID-19 test methods and devices and proposed performance criteria. EC; Brussels: 2020, https://ec.europa.eu/docsroom/documents/40805. Accessed January 2, 2021. | |
Taleghani N and Taghipour F: Diagnosis of COVID-19 for controlling the pandemic: A review of the state-of-the-art. Biosens Bioelectron. 174:1128302021. View Article : Google Scholar | |
Jung J, Jang H, Kim HK, Kim J, Kim A and Ko KP: The Importance of Mandatory COVID-19 Diagnostic Testing Prior to Release from Quarantine. J Korean Med Sci. 35:e3142020. View Article : Google Scholar : PubMed/NCBI | |
Winter AK and Hegde ST: The important role of serology for COVID-19 control. Lancet Infect Dis. 20:758–759. 2020. View Article : Google Scholar : PubMed/NCBI | |
Vandenberg O, Martiny D, Rochas O, van Belkum A and Kozlakidis Z: Considerations for diagnostic COVID-19 tests. Nat Rev Microbiol. 19:171–183. 2021. View Article : Google Scholar | |
Tromberg BJ, Schwetz TA, Pérez-Stable EJ, Hodes RJ, Woychik RP, Bright RA, Fleurence RL and Collins FS: Rapid scaling up of Covid-19 diagnostic testing in the United States - The NIH RADx Initiative. N Engl J Med. 383:1071–1077. 2020. View Article : Google Scholar : PubMed/NCBI | |
Guglielmi G: The explosion of new coronavirus tests that could help to end the pandemic. Nature. 583:506–509. 2020. View Article : Google Scholar : PubMed/NCBI | |
Tsatsakis A, Calina D, Falzone L, Petrakis D, Mitrut R, Siokas V, Pennisi M, Lanza G, Libra M, Doukas SG, et al: SARS-CoV-2 pathophysiology and its clinical implications: An integrative overview of the pharmacotherapeutic management of COVID-19. Food Chem Toxicol. 146:1117692020. View Article : Google Scholar : PubMed/NCBI | |
Alpdagtas S, Ilhan E, Uysal E, Sengor M, Ustundag CB and Gunduz O: Evaluation of current diagnostic methods for COVID-19. APL Bioeng. 4:0415062020. View Article : Google Scholar : PubMed/NCBI | |
Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, Tao ZW, Tian JH, Pei YY, et al: A new coronavirus associated with human respiratory disease in China. Nature. 579:265–269. 2020. View Article : Google Scholar : PubMed/NCBI | |
Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, et al: China novel coronavirus investigating and research team: a novel coronavirus from patients with pneumonia in China 2019. N Engl J Med. 382:727–733. 2020. View Article : Google Scholar : PubMed/NCBI | |
Kumar R, Nagpal S, Kaushik S and Mendiratta S: COVID-19 diagnostic approaches: Different roads to the same destination. Virusdisease. 31:97–105. 2020. View Article : Google Scholar : PubMed/NCBI | |
Brun AL, Gence-Breney A, Trichereau J, Ballester MC, Vasse M, Chabi ML, Mellot F and Grenier PA: COVID-19 pneumonia: High diagnostic accuracy of chest CT in patients with intermediate clinical probability. Eur Radiol. 31:1969–1977. 2021. View Article : Google Scholar | |
Martinez RM: Clinical samples for SARS-CoV-2 detection: Review of the early literature. Clin Microbiol Newsl. 42:121–127. 2020. View Article : Google Scholar : PubMed/NCBI | |
Rao GG, Agarwal A and Batura D: Testing times in Coronavirus disease (COVID-19): A tale of two nations. Med J Armed Forces India. 76:243–249. 2020. View Article : Google Scholar : PubMed/NCBI | |
Jarvis KF and Kelley JB: Temporal dynamics of viral load and false negative rate influence the levels of testing necessary to combat COVID19 spread. medRxiv. View Article : Google Scholar | |
Rode OĐ, Kurolt IC, Puljiz I, Čivljak R, Balent NC, Laškaj R, Tiljak MK, Mikulić R and Markotić A: Antibody response and the clinical presentation of patients with COVID-19 in Croatia: The importance of a two-step testing approach. Eur J Clin Microbiol Infect Dis. 40:261–268. 2021. View Article : Google Scholar | |
Bisoffi Z, Pomari E, Deiana M, Piubelli C, Ronzoni N, Beltrame A, Bertoli G, Riccardi N, Perandin F, Formenti F, et al: Sensitivity, specificity and predictive values of molecular and serological tests for COVID-19: a longitudinal study in emergency room. Diagnostics (Basel). 10:6692020. View Article : Google Scholar | |
Krajewski R, Gołębiowska J, Makuch S, Mazur G and Agrawal S: Update on serologic testing in COVID-19. Clin Chim Acta. 510:746–750. 2020. View Article : Google Scholar : PubMed/NCBI | |
Guo L, Ren L, Yang S, Xiao M, Chang D, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, et al: Profiling early humoral response to diagnose novel coronavirus disease (COVID-19). Clin Infect Dis. 71:778–785. 2020. View Article : Google Scholar : PubMed/NCBI | |
Chirumbolo S: Swabbing Thoroughly People for COVID-19 Positivity. Insights on the Main Bio-analytical and Microbiology Bias and Concerns. Curr Microbiol. 77:3680–3684. 2020. View Article : Google Scholar : PubMed/NCBI | |
Lippi G, Simundic AM and Plebani M: Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19). Clin Chem Lab Med. 58:1070–1076. 2020. View Article : Google Scholar : PubMed/NCBI | |
Ora J, Puxeddu E, Cavalli F, Giorgino FM, Girolami A, Chiocchi M, Sergiacomi G, Federici M and Rogliani P: Does bronchoscopy help the diagnosis in COVID-19 infection? Eur Respir J. 56:20016192020. View Article : Google Scholar : PubMed/NCBI | |
Tong Y, Bao A, Chen H, Huang J, Lv Z, Feng L, Cheng Y, Wang Y, Bai L, Rao W, et al: Necessity for detection of SARS-CoV-2 RNA in multiple types of specimens for the discharge of the patients with COVID-19. J Transl Med. 18:4112020. View Article : Google Scholar : PubMed/NCBI | |
McFee DRB: COVID-19 Laboratory Testing/CDC Guidelines. Dis Mon. 66:1010672020. View Article : Google Scholar : PubMed/NCBI | |
Fu Y, Li Y, Guo E, He L, Liu J, Yang B, Li F, Wang Z, Li Y, Xiao R, et al: Dynamics and correlation among viral positivity, seroconversion, and disease severity in COVID-19: a retrospective study. Ann Intern Med. 8:M20–M3337. 2020. | |
Mesoraca A, Margiotti K, Viola A, Cima A, Sparacino D and Giorlandino C: Evaluation of SARS-CoV-2 viral RNA in fecal samples. Virol J. 17:862020. View Article : Google Scholar : PubMed/NCBI | |
Ng SC and Tilg H: COVID-19 and the gastrointestinal tract: More than meets the eye. Gut. 69:973–974. 2020. View Article : Google Scholar : PubMed/NCBI | |
Long DR, Gombar S, Hogan CA, Greninger AL, O'Reilly-Shah V, Bryson-Cahn C, Stevens B, Rustagi A, Jerome KR, Kong CS, et al: Occurrence and timing of subsequent severe acute respiratory syndrome coronavirus 2 reverse-transcription polymerase chain reaction positivity among initially negative patients. Clin Infect Dis. 72:323–326. 2021. View Article : Google Scholar : PubMed/NCBI | |
Mahendiratta S, Batra G, Sarma P, Kumar H, Bansal S, Kumar S, Prakash A, Sehgal R and Medhi B: Molecular diagnosis of COVID-19 in different biologic matrix, their diagnostic validity and clinical relevance: A systematic review. Life Sci. 258:1182072020. View Article : Google Scholar : PubMed/NCBI | |
da Silva SJR, Silva CTAD, Guarines KM, Mendes RPG, Pardee K, Kohl A and Pena L: Clinical and Laboratory Diagnosis of SARS-CoV-2, the Virus Causing COVID-19. ACS Infect Dis. 6:2319–2336. 2020. View Article : Google Scholar : PubMed/NCBI | |
Li D, Zhang J and Li J: Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2. Theranostics. 10:7150–7162. 2020. View Article : Google Scholar : PubMed/NCBI | |
Chan JF, Kok KH, Zhu Z, Chu H, To KK, Yuan S and Yuen KY: Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 9:221–236. 2020. View Article : Google Scholar : PubMed/NCBI | |
Centers for Disease Control and Prevention (CDC): CDC's Diagnostic Test for COVID-19 Only and Supplies. https://www.cdc.gov/coronavirus/2019-ncov/lab/virus-requests.html. Accessed January 5, 2021. | |
Centers for Disease Control and Prevention (CDC): Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes. https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html. Accessed January 5, 2021. | |
Dorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, et al: Lower cost alternatives for molecular diagnosis of COVID-19: Conventional RT-PCR and SYBR Green-based RT-qPCR. Braz J Microbiol. 51:1117–1123. 2020. View Article : Google Scholar : PubMed/NCBI | |
Neilan AM, Losina E, Bangs AC, Flanagan C, Panella C, Eskibozkurt GE, Mohareb A, Hyle EP, Scott JA, Weinstein MC, et al: Clinical Impact, Costs, and Cost-Effectiveness of Expanded SARS-CoV-2 Testing in Massachusetts. Clin Infect Dis. Sep 18–2020.Epub ahead of print. View Article : Google Scholar | |
Smyrlaki I, Ekman M, Lentini A, Rufino de Sousa N, Papanicolaou N, Vondracek M, Aarum J, Safari H, Muradrasoli S, Rothfuchs AG, et al: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR. Nat Commun. 11:48122020. View Article : Google Scholar : PubMed/NCBI | |
U.S Food & Drug: COVID-19 tests and collection kits authorized by the FDA in 2020: Infographic. https://www.fda.gov/medical-devices/coronavirus-covid-19-and-medical-devices/covid-19-tests-and-collection-kits-authorized-fda-2020-infographic. Accessed January 8, 2021. | |
Fomsgaard AS and Rosenstierne MW: An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020. Euro Surveill. 25:20003982020. View Article : Google Scholar | |
Udugama B, Kadhiresan P, Kozlowski HN, Malekjahani A, Osborne M, Li VYC, Chen H, Mubareka S, Gubbay JB and Chan WCW: Diagnosing COVID-19: The Disease and Tools for Detection. ACS Nano. 14:3822–3835. 2020. View Article : Google Scholar : PubMed/NCBI | |
Ravi N, Cortade DL, Ng E and Wang SX: Diagnostics for SARS-CoV-2 detection: A comprehensive review of the FDA-EUA COVID-19 testing landscape. Biosens Bioelectron. 165:1124542020. View Article : Google Scholar : PubMed/NCBI | |
Wells CR, Townsend JP, Pandey A, Moghadas SM, Krieger G, Singer B, McDonald RH, Fitzpatrick MC and Galvani AP: Optimal COVID-19 quarantine and testing strategies. Nat Commun. 12:3562021. View Article : Google Scholar : PubMed/NCBI | |
Bustin SA and Nolan T: RT-qPCR Testing of SARS-CoV-2: A Primer. Int J Mol Sci. 21:30042020. View Article : Google Scholar : | |
Böger B, Fachi MM, Vilhena RO, Cobre AF, Tonin FS and Pontarolo R: Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19. Am J Infect Control. 49:21–29. 2021. View Article : Google Scholar | |
Kucirka LM, Lauer SA, Laeyendecker O, Boon D and Lessler J: Variation in false-negative rate of reverse transcriptase polymerase chain reaction-based SARS-CoV-2 tests by time since exposure. Ann Intern Med. 173:262–267. 2020. View Article : Google Scholar : PubMed/NCBI | |
Afzal A: Molecular diagnostic technologies for COVID-19: Limitations and challenges. J Adv Res. 26:149–159. 2020. View Article : Google Scholar : PubMed/NCBI | |
Tahamtan A and Ardebili A: Real-time RT-PCR in COVID-19 detection: Issues affecting the results. Expert Rev Mol Diagn. 20:453–454. 2020. View Article : Google Scholar : PubMed/NCBI | |
Pan Y, Long L, Zhang D, Yuan T, Cui S, Yang P, Wang Q and Ren S: Potential false-negative nucleic acid testing results for severe acute respiratory syndrome coronavirus 2 from thermal inactivation of samples with low viral loads. Clin Chem. 66:794–801. 2020. View Article : Google Scholar : PubMed/NCBI | |
Li Y, Yao L, Li J, Chen L, Song Y, Cai Z and Yang C: Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19. J Med Virol. 92:903–908. 2020. View Article : Google Scholar : PubMed/NCBI | |
World Health Organization (WHO): Recommendations for laboratories testing by PCR for presence of SARS coronavirus-RNA. WHO; Geneva: 2020, https://www.who.int/csr/sars/coronarec-ommendations/en/. Accessed January 26, 2021. | |
Cerutti F, Burdino E, Milia MG, Allice T, Gregori G, Bruzzone B and Ghisetti V: Urgent need of rapid tests for SARS CoV-2 antigen detection: Evaluation of the SD-Biosensor antigen test for SARS-CoV-2. J Clin Virol. 132:1046542020. View Article : Google Scholar : PubMed/NCBI | |
Olalekan A, Iwalokun B, Akinloye OM, Popoola O, Samuel TA and Akinloye O: COVID-19 rapid diagnostic test could contain transmission in low- and middle-income countries. Afr J Lab Med. 9:12552020. View Article : Google Scholar : PubMed/NCBI | |
Augustine R, Das S, Hasan ASA, Abdul Salam S, Augustine P, Dalvi YB, Varghese R, Primavera R, Yassine HM, et al: Rapid antibody-based COVID-19 mass surveillance: relevance, challenges, and prospects in a pandemic and post-pandemic world. J Clin Med. 9:33722020. View Article : Google Scholar : | |
Albert E, Torres I, Bueno F, Huntley D, Molla E, Fernández-Fuentes MÁ, Martínez M, Poujois S, Forqué L, Valdivia A, et al: Field evaluation of a rapid antigen test (Panbio™ COVID-19 Ag Rapid Test Device) for COVID-19 diagnosis in primary healthcare centres. Clin Microbiol Infect. 27:472.e7–472.e10. 2021. View Article : Google Scholar | |
Pilarowski G, Lebel P, Sunshine S, Liu J, Crawford E, Marquez C, Rubio L, Chamie G, Martinez J, Peng J, et al: Performance characteristics of a rapid SARS-CoV-2 antigen detection assay at a public plaza testing site in San Francisco. J Infect Dis. Jan 4–2021.Epub ahead of print. View Article : Google Scholar : | |
Lee JH, Choi M, Jung Y, Lee SK, Lee CS, Kim J, Kim J, Kim NH, Kim BT and Kim HG: A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2). Biosens Bioelectron. 171:1127152021. View Article : Google Scholar | |
Diao B, Wen K, Zhang J, Chen J, Han C, Chen Y, Wang S, Deng G, Zhou H and Wu Y: Accuracy of a nucleocapsid protein antigen rapid test in the diagnosis of SARS-CoV-2 infection. Clin Microbiol Infect. 27:289.e1–289.e4. 2021. View Article : Google Scholar | |
Carter LJ, Garner LV, Smoot JW, Li Y, Zhou Q, Saveson CJ, Sasso JM, Gregg AC, Soares DJ, Beskid TR, et al: Assay Techniques and Test Development for COVID-19 Diagnosis. ACS Cent Sci. 6:591–605. 2020. View Article : Google Scholar : PubMed/NCBI | |
Huang C, Wen T, Shi FJ, Zeng XY and Jiao YJ: Rapid detection of igm antibodies against the SARS-CoV-2 virus via colloidal gold nanoparticle-based lateral-flow assay. ACS Omega. 5:12550–12556. 2020. View Article : Google Scholar : PubMed/NCBI | |
Mak GC, Cheng PK, Lau SS, Wong KK, Lau CS, Lam ET, Chan RC and Tsang DN: Evaluation of rapid antigen test for detection of SARS-CoV-2 virus. J Clin Virol. 129:1045002020. View Article : Google Scholar : PubMed/NCBI | |
Lanser L, Bellmann-Weiler R, Öttl KW, Huber L, Griesmacher A, Theurl I and Weiss G: Evaluating the clinical utility and sensitivity of SARS-CoV-2 antigen testing in relation to RT-PCR Ct values. Infection. 13:1–3. 2020. | |
Dinnes J, Deeks JJ, Adriano A, Berhane S, Davenport C, Dittrich S, Emperador D, Takwoingi Y, Cunningham J, Beese S, et al: Cochrane COVID-19 Diagnostic Test Accuracy Group: Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection. Cochrane Database Syst Rev. 8:CD0137052020. | |
Montesinos I, Gruson D, Kabamba B, Dahma H, Van den Wijngaert S, Reza S, Carbone V, Vandenberg O, Gulbis B, Wolff F, et al: Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies. J Clin Virol. 128:1044132020. View Article : Google Scholar : PubMed/NCBI | |
Prazuck T, Colin M, Giachè S, Gubavu C, Seve A, Rzepecki V, Chevereau-Choquet M, Kiani C, Rodot V, Lionnet E, et al: Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. PLoS One. 15:e02376942020. View Article : Google Scholar : PubMed/NCBI | |
Jacobs J, Kühne V, Lunguya O, Affolabi D, Hardy L and Vandenberg O: Implementing COVID-19 (SARS-CoV-2) rapid diagnostic tests in Sub-Saharan Africa: a review. Front Med (Lausanne). 7:5577972020. View Article : Google Scholar | |
La Rosa Fabián C and Urquizo Briceño L: Anti-SARS-Cov-2 IgA in Current Scenario of IgM and IgG Rapid Test: A New Alternative for the Diagnostic of COVID-19. SN Compr Clin Med. 26:1–3. 2020. | |
Canetti D, Dell'Acqua R, Riccardi N, Della Torre L, Bigoloni A, Muccini C, Bruzzesi E, Ranzenigo M, Chiurlo M, Racca S, et al: SARS-CoV-2 IgG/IgM Rapid Test as a Diagnostic Tool in Hospitalized Patients and Healthcare Workers, at a large Teaching Hospital in northern Italy, during the 2020 COVID-19 Pandemic. New Microbiol. 43:161–165. 2020.PubMed/NCBI | |
Alhajj M and Farhana A: Enzyme Linked Immunosorbent Assay. StatPearls. StatPearls Publishing; Treasure Island, FL: 2020 | |
Tré-Hardy M, Wilmet A, Beukinga I, Favresse J, Dogné JM, Douxfils J and Blairon L: Analytical and clinical validation of an ELISA for specific SARS-CoV-2 IgG, IgA, and IgM anti-bodies. J Med Virol. 93:803–811. 2021. View Article : Google Scholar | |
Freeman B, Lester S, Mills L, Rasheed MAU, Moye S, Abiona O, Hutchinson GB, Morales-Betoulle M, Krapinunaya I, Gibbons A, et al: Validation of a SARS-CoV-2 spike protein ELISA for use in contact investigations and serosurveillance. bioRxiv. View Article : Google Scholar | |
Korth J, Wilde B, Dolff S, Anastasiou OE, Krawczyk A, Jahn M, Cordes S, Ross B, Esser S, Lindemann M, et al: SARS-CoV-2-specific antibody detection in healthcare workers in Germany with direct contact to COVID-19 patients. J Clin Virol. 128:1044372020. View Article : Google Scholar : PubMed/NCBI | |
Alharbi SA, Almutairi AZ, Jan AA and Alkhalify AM: Enzyme-linked immunosorbent assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM/IgA and IgG antibodies among healthcare workers. Cureus. 12:e102852020.PubMed/NCBI | |
Stringhini S, Wisniak A, Piumatti G, Azman AS, Lauer SA, Baysson H, De Ridder D, Petrovic D, Schrempft S, Marcus K, et al: Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva, Switzerland (SEROCoV-POP): A population-based study. Lancet. 396:313–319. 2020. View Article : Google Scholar : PubMed/NCBI | |
Vashist SK: In vitro diagnostic assays for COVID-19: recent advances and emerging trends. Diagnostics (Basel). 10:2022020. View Article : Google Scholar | |
Tanaka Y, Kamimura K, Nakamura R, Ohkoshi-Yamada M, Koseki Y, Mizusawa T, Ikarashi S, Hayashi K, Sato H, Sakamaki A, et al: Usefulness of ultrasonography to assess the response to steroidal therapy for the rare case of type 2b immunoglobulin G4-related sclerosing cholangitis without pancreatitis: A case report. World J Clin Cases. 8:5821–5830. 2020. View Article : Google Scholar : PubMed/NCBI | |
AvŞar T, ÇaliŞ Ş, Yilmaz B, Demİrcİ OtluoĞlu G, Holyavkİn C and Kili CT: Genome-wide identification of Chiari malformation type I associated candidate genes and chromosomal variations. Turk J Biol. 44:449–456. 2020. View Article : Google Scholar | |
La Marca A, Capuzzo M, Paglia T, Roli L, Trenti T and Nelson SM: Testing for SARS-CoV-2 (COVID-19): A systematic review and clinical guide to molecular and serological in-vitro diagnostic assays. Reprod Biomed Online. 41:483–499. 2020. View Article : Google Scholar : PubMed/NCBI | |
Sheikhzadeh E, Eissa S, Ismail A and Zourob M: Diagnostic techniques for COVID-19 and new developments. Talanta. 220:1213922020. View Article : Google Scholar : PubMed/NCBI | |
Zhu N, Wang W, Liu Z, Liang C, Wang W, Ye F, Huang B, Zhao L, Wang H, Zhou W, et al: Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. Nat Commun. 11:39102020. View Article : Google Scholar : PubMed/NCBI | |
Leland DS and Ginocchio CC: Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev. 20:49–78. 2007. View Article : Google Scholar : PubMed/NCBI | |
Jain R, Sarkale P, Mali D, Shete AM, Patil DY, Majumdar T, Suryawanshi A, Patil S, Mohandas S and Yadav PD: Inactivation of SARS-CoV-2 by gamma irradiation. Indian J Med Res. Nov 27–2020.Epub ahead of print. PubMed/NCBI | |
Park WB, Kwon NJ, Choi SJ, Kang CK, Choe PG, Kim JY, Yun J, Lee GW, Seong MW, Kim NJ, et al: Virus Isolation from the First Patient with SARS-CoV-2 in Korea. J Korean Med Sci. 35:e842020. View Article : Google Scholar : PubMed/NCBI | |
Zhao J, Zhou H, Huang W, Zhou J, Qiu M, Deng Z, Chen L, Weng Y, Cai L, Gu Y, et al: Cell morphological analysis of SARS-CoV-2 infection by transmission electron microscopy. J Thorac Dis. 12:4368–4373. 2020. View Article : Google Scholar : PubMed/NCBI | |
Akilesh S, Nicosia RF, Alpers CE, Tretiakova M, Hsiang TY, Gale M Jr and Smith KD: Characterizing viral infection by electron microscopy: lessons from the coronavirus disease 2019 pandemic. Am J Pathol. 191:222–227. 2021. View Article : Google Scholar | |
Goldsmith CS, Tatti KM, Ksiazek TG, Rollin PE, Comer JA, Lee WW, Rota PA, Bankamp B, Bellini WJ and Zaki SR: Ultrastructural characterization of SARS coronavirus. Emerg Infect Dis. 10:320–326. 2004. View Article : Google Scholar : PubMed/NCBI | |
Bain W, Lee JS, Watson AM and Stitt-Fischer MS: Practical guidelines for collection, manipulation and inactivation of SARS-CoV-2 and COVID-19 clinical specimens. Curr Protoc Cytom. 93:e772020.PubMed/NCBI | |
Wu SY, Yau HS, Yu MY, Tsang HF, Chan LWC, Cho WCS, Shing Yu AC, Yuen Yim AK, Li MJW, Wong YKE, et al: The diagnostic methods in the COVID-19 pandemic, today and in the future. Expert Rev Mol Diagn. 20:985–993. 2020. View Article : Google Scholar : PubMed/NCBI | |
Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, Xing F, Liu J, Yip CC, Poon RW, et al: A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: A study of a family cluster. Lancet. 395:514–523. 2020. View Article : Google Scholar : PubMed/NCBI | |
Wang M, Fu A, Hu B, Tong Y, Liu R, Liu Z, Gu J, Xiang B, Liu J, Jiang W, et al: Nanopore targeted sequencing for the accurate and comprehensive detection of SARS-CoV-2 and other respiratory viruses. Small. 16:e20021692020. View Article : Google Scholar : PubMed/NCBI | |
Addetia A, Xie H, Roychoudhury P, Shrestha L, Loprieno M, Huang ML, Jerome KR and Greninger AL: Identification of multiple large deletions in ORF7a resulting in in-frame gene fusions in clinical SARS-CoV-2 isolates. J Clin Virol. 129:1045232020. View Article : Google Scholar : PubMed/NCBI | |
Di Giallonardo F, Duchene S, Puglia I, Curini V, Profeta F, Cammà C, Marcacci M, Calistri P, Holmes EC and Lorusso A: Genomic Epidemiology of the First Wave of SARS-CoV-2 in Italy. Viruses. 12:14382020. View Article : Google Scholar : | |
Moore SC, Penrice-Randal R, Alruwaili M, Randle N, Armstrong S, Hartley C, Haldenby S, Dong X, Alrezaihi A, Almsaud M, et al: Amplicon-Based Detection and Sequencing of SARS-CoV-2 in Nasopharyngeal Swabs from Patients With COVID-19 and Identification of Deletions in the Viral Genome That Encode Proteins Involved in Interferon Antagonism. Viruses. 12:11642020. View Article : Google Scholar : | |
Kames J, Holcomb DD, Kimchi O, DiCuccio M, Hamasaki-Katagiri N, Wang T, Komar AA, Alexaki A and Kimchi-Sarfaty C: Sequence analysis of SARS-CoV-2 genome reveals features important for vaccine design. Sci Rep. 10:156432020. View Article : Google Scholar : PubMed/NCBI | |
Conti P, Caraffa A, Gallenga CE, Kritas SK, Frydas I, Younes A, Di Emidio P, Tetè G, Pregliasco F and Ronconi G: The British variant of the new coronavirus-19 (Sars-Cov-2) should not create a vaccine problem. J Biol Regul Homeost Agents. 35:1–4. 2021. | |
Hourdel V, Kwasiborski A, Balière C, Matheus S, Batéjat CF, Manuguerra JC, Vanhomwegen J and Caro V: rapid genomic characterization of SARS-CoV-2 by direct amplicon-based sequencing through comparison of MinION and Illumina iSeq100TM system. Front Microbiol. 11:5713282020. View Article : Google Scholar : | |
illumina: Comprehensive Workf low for Detecting Coronavirus Using Illumina Benchtop Systems - A Shotgun Metagenomics Sequencing Workflow for Effective Detection and Characterization of Coronavirus Strains. https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/ngs-coronavirus-app-note-1270-2020-001.pdf. Accessed January 20, 2021. | |
Oxford Nanopore Technologies MinION - Powerful, real-time, long-read sequencing in the palm of your hand. https://nanoporetech.com/sites/default/files/s3/literature/MinION-Mk1C-brochure.pdf. Accessed January 20, 2021. | |
Meredith LW, Hamilton WL, Warne B, Houldcroft CJ, Hosmillo M, Jahun AS, Curran MD, Parmar S, Caller LG, Caddy SL, et al: Rapid implementation of SARS-CoV-2 sequencing to investigate cases of health-care associated COVID-19: A prospective genomic surveillance study. Lancet Infect Dis. 20:1263–1271. 2020. View Article : Google Scholar : PubMed/NCBI | |
Wen F, Yu H, Guo J, Li Y, Luo K and Huang S: Identification of the hyper-variable genomic hotspot for the novel coronavirus SARS-CoV-2. J Infect. 80:671–693. 2020. View Article : Google Scholar : PubMed/NCBI | |
Lam TT: Tracking the Genomic Footprints of SARS-CoV-2 Transmission. Trends Genet. 36:544–546. 2020. View Article : Google Scholar : PubMed/NCBI | |
Landete P, Quezada Loaiza CA, Aldave-Orzaiz B, Muñiz SH, Maldonado A, Zamora E, Sam Cerna AC, Del Cerro E, Alonso RC and Couñago F: Clinical features and radiological manifestations of COVID-19 disease. World J Radiol. 12:247–260. 2020. View Article : Google Scholar : PubMed/NCBI | |
Chen H, Ai L, Lu H and Li H: Clinical and imaging features of COVID-19. Radiol Infect Dis. 7:43–50. 2020. View Article : Google Scholar : PubMed/NCBI | |
Al-Qahtani M, AlAli S, AbdulRahman A, Salman Alsayyad A, Otoom S and Atkin SL: The prevalence of asymptomatic and symptomatic COVID-19 in a cohort of quarantined subjects. Int J Infect Dis. 102:285–288. 2021. View Article : Google Scholar | |
Yanes-Lane M, Winters N, Fregonese F, Bastos M, Perlman-Arrow S, Campbell JR and Menzies D: Proportion of asymptomatic infection among COVID-19 positive persons and their transmission potential: A systematic review and meta-analysis. PLoS One. 15:e02415362020. View Article : Google Scholar : PubMed/NCBI | |
Katopodis P, Kerslake R, Davies J, Randeva HS, Chatha K, Hall M, Spandidos DA, Anikin V, Polychronis A, Robertus JL, et al: COVID 19 and SARS CoV 2 host cell entry mediators: Expression profiling of TMRSS4 in health and disease. Int J Mol Med. 47:642021. View Article : Google Scholar | |
Vivarelli S, Falzone L, Torino F, Scandurra G, Russo G, Bordonaro R, Pappalardo F, Spandidos DA, Raciti G and Libra M: Immune-checkpoint inhibitors from cancer to COVID-19: A promising avenue for the treatment of patients with COVID-19 (Review). Int J Oncol. 58:145–157. 2021. View Article : Google Scholar : PubMed/NCBI | |
Vivarelli S, Falzone L, Grillo CM, Scandurra G, Torino F and Libra M: Cancer Management during COVID-19 Pandemic: Is Immune Checkpoint Inhibitors-Based Immunotherapy Harmful or Beneficial? Cancers (Basel). 12:22372020. View Article : Google Scholar | |
Abdi A, Jalilian M, Sarbarzeh PA and Vlaisavljevic Z: Diabetes and COVID-19: A systematic review on the current evidences. Diabetes Res Clin Pract. 166:1083472020. View Article : Google Scholar : PubMed/NCBI | |
Huang S, Wang J, Liu F, Liu J, Cao G, Yang C, Liu W, Tu C, Zhu M and Xiong B: COVID-19 patients with hypertension have more severe disease: A multicenter retrospective observational study. Hyperte Res. 43:824–831. 2020. View Article : Google Scholar | |
Pascarella G, Strumia A, Piliego C, Bruno F, Del Buono R, Costa F, Scarlata S and Agrò FE: COVID-19 diagnosis and management: A comprehensive review. J Intern Med. 288:192–206. 2020. View Article : Google Scholar : PubMed/NCBI | |
Torre La G, Massetti AP, Antonelli G, Fimiani C, Fantini M, Marte M, Faticoni A, Previte CM, Turriziani O, Pugliese F, et al: Covid-Sapienza Collaborative Group: Anosmia and Ageusia as Predictive Signs of COVID-19 in Healthcare Workers in Italy: A Prospective Case-Control Study. J Clin Med. 9:28702020. View Article : Google Scholar | |
Tanasa IA, Manciuc C, Carauleanu A, Navolan DB, Bohiltea RE and Nemescu D: Anosmia and ageusia associated with coronavirus infection (COVID-19) - what is known? Exp Ther Med. 20:2344–2347. 2020.PubMed/NCBI | |
Pennisi M, Lanza G, Falzone L, Fisicaro F, Ferri R and Bella R: SARS-CoV-2 and the Nervous System: From Clinical Features to Molecular Mechanisms. Int J Mol Sci. 21:54752020. View Article : Google Scholar : | |
Connors JM and Levy JH: COVID-19 and its implications for thrombosis and anticoagulation. Blood. 135:2033–2040. 2020. View Article : Google Scholar : PubMed/NCBI | |
Diao B, Wang C, Tan Y, Chen X, Liu Y, Ning L, Chen L, Li M, Liu Y, Wang G, et al: Reduction and functional exhaustion of T cells in patients with coronavirus disease 2019 (COVID-19). Front Immunol. 11:8272020. View Article : Google Scholar : PubMed/NCBI | |
Basu-Ray I, Almaddah Nk, Adeboye A and Soos MP: Cardiac Manifestations Of Coronavirus. StatPearls. StatPearls Publishing; Treasure Island, FL: 2020 | |
Garland V, Kumar AB and Borum ML: Gastrointestinal and hepatic manifestations of COVID-19: evolving recognition and need for increased understanding in vulnerable populations. J Natl Med Assoc. Aug 15–2020.Epub ahead of print. | |
Baj J, Karakuła-Juchnowicz H, Teresiński G, Buszewicz G, Ciesielka M, Sitarz E, Forma A, Karakuła K, Flieger W, Portincasa P, et al: COVID-19: Specific and non-specific clinical manifestations and symptoms: the current state of knowledge. J Clin Med. 9:17532020. View Article : Google Scholar : | |
Renu K, Prasanna PL and Valsala Gopalakrishnan A: Coronaviruses pathogenesis, comorbidities and multi-organ damage - A review. Life Sci. 255:1178392020. View Article : Google Scholar : PubMed/NCBI | |
Chang MC, Lee W, Hur J and Park D: Chest Computed Tomography Findings in Asymptomatic Patients with COVID-19. Respiration. 99:748–754. 2020. View Article : Google Scholar : PubMed/NCBI | |
Jędrusik P, Gaciong Z, Sklinda K, Sierpiński R, Walecki J and Gujski M: Diagnostic role of chest computed tomography in coronavirus disease 2019. Pol Arch Intern Med. 130:520–528. 2020. | |
Kang EY, Staples CA, McGuinness G, Primack SL and Müller NL: Detection and differential diagnosis of pulmonary infections and tumors in patients with AIDS: Value of chest radiography versus CT. AJR Am J Roentgenol. 166:15–19. 1996. View Article : Google Scholar : PubMed/NCBI | |
Larici AR, Cicchetti G, Marano R, Merlino B, Elia L, Calandriello L, Del Ciello A, Farchione A, Savino G, Infante A, et al: Multimodality imaging of COVID-19 pneumonia: From diagnosis to follow-up. A comprehensive review. Eur J Radiol. 131:1092172020. View Article : Google Scholar : PubMed/NCBI | |
Ippolito D, Maino C, Pecorelli A, Allegranza P, Cangiotti C, Capodaglio C, Mariani I, Giandola T, Gandola D, Bianco I, et al: Chest X-ray features of SARS-CoV-2 in the emergency department: A multicenter experience from northern Italian hospitals. Respir Med. 170:1060362020. View Article : Google Scholar : PubMed/NCBI | |
Li P, Chen L, Liu Z, Pan J, Zhou D, Wang H, Gong H, Fu Z, Song Q, Min Q, et al: Clinical features and short-term outcomes of elderly patients with COVID-19. Int J Infect Dis. 97:245–250. 2020. View Article : Google Scholar : PubMed/NCBI | |
Cui N, Zou X and Xu L: Preliminary CT findings of coronavirus disease 2019 (COVID-19). Clin Imaging. 65:124–132. 2020. View Article : Google Scholar : PubMed/NCBI | |
Kovács A, Palásti P, Veréb D, Bozsik B, Palkó A and Kincses ZT: The sensitivity and specificity of chest CT in the diagnosis of COVID-19. Eur Radiol. 13:1–6. 2020. | |
Zhao W, He L, Tang H, Xie X, Tang L and Liu J: The Relationship Between Chest Imaging Findings and the Viral Load of COVID-19. Front Med (Lausanne). 7:5585392020. View Article : Google Scholar | |
Meng H, Xiong R, He R, Lin W, Hao B, Zhang L, Lu Z, Shen X, Fan T, Jiang W, et al: CT imaging and clinical course of asymptomatic cases with COVID-19 pneumonia at admission in Wuhan, China. J Infect. 81:e33–e39. 2020. View Article : Google Scholar : PubMed/NCBI | |
Mehrabi S, Fontana S, Mambrin F, Nguyen HQ, Righi E, Tacconelli E and Mansueto G: Pitfalls of computed tomography in the coronavirus 2019 (COVID-19) era: a new perspective on ground-glass opacities. Cureus. 12:e81512020.PubMed/NCBI | |
Tofighi S, Najafi S, Johnston SK and Gholamrezanezhad A: Low-dose CT in COVID-19 outbreak: Radiation safety, image wisely, and image gently pledge. Emerg Radiol. 27:601–605. 2020. View Article : Google Scholar : PubMed/NCBI | |
Qiu G, Gai Z, Tao Y, Schmitt J, Kullak-Ublick GA and Wang J: Dual-functional plasmonic photothermal biosensors for highly accurate severe acute respiratory syndrome coronavirus 2 detection. ACS Nano. 14:5268–5277. 2020. View Article : Google Scholar : PubMed/NCBI | |
Pandey LM: Design of engineered surfaces for prospective detection of SARS-CoV-2 using quartz crystal micro-balance-based techniques. Expert Rev Proteomics. 17:425–432. 2020. View Article : Google Scholar : PubMed/NCBI | |
Maddali H, Miles CE, Kohn J and O'Carroll DM: Optical Biosensors for Virus Detection: Prospects for SARS-CoV-2/COVID-19. ChemBioChem. 22:1176–1189. 2021. View Article : Google Scholar | |
Cui F and Zhou HS: Diagnostic methods and potential portable biosensors for coronavirus disease 2019. Biosens Bioelectron. 165:1123492020. View Article : Google Scholar : PubMed/NCBI | |
Samson R, Navale GR and Dharne MS: Biosensors: frontiers in rapid detection of COVID-19. 3 Biotech. 10:3852020. | |
Seo G, Lee G, Kim MJ, Baek SH, Choi M, Ku KB, Lee CS, Jun S, Park D, Kim HG, et al: Rapid detection of COVID-19 causative virus (SARS-CoV-2) in human nasopharyngeal swab specimens using field-effect transistor-based biosensor. ACS Nano. 14:5135–5142. 2020. View Article : Google Scholar : PubMed/NCBI | |
Zhao H, Liu F, Xie W, Zhou TC, OuYang J, Jin L, Li H, Zhao CY, Zhang L, Wei J, et al: Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone. Sens Acuators B Chem. 327:1288992021. View Article : Google Scholar | |
Choi JR: Development of point-of-care biosensors for COVID-19. Front Chem. 8:5172020. View Article : Google Scholar : PubMed/NCBI | |
Lu R, Wu X, Wan Z, Li Y, Jin X and Zhang C: A novel reverse transcription loop-mediated isothermal amplification method for rapid detection of SARS-CoV-2. Int J Mol Sci. 21:28262020. View Article : Google Scholar : | |
Yuan X, Yang C, He Q, Chen J, Yu D, Li J, Zhai S, Qin Z, Du K, Chu Z, et al: Current and perspective diagnostic techniques for COVID-19. ACS Infect Dis. 6:1998–2016. 2020. View Article : Google Scholar : PubMed/NCBI | |
Huang WE, Lim B, Hsu CC, Xiong D, Wu W, Yu Y, Jia H, Wang Y, Zeng Y, Ji M, et al: RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2. Microb Biotechnol. 13:950–961. 2020. View Article : Google Scholar : PubMed/NCBI | |
Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, et al: CRISPR-Cas12-based detection of SARS-CoV-2. Nat Biotechnol. 38:870–874. 2020. View Article : Google Scholar : PubMed/NCBI | |
Song J, Liu C, Mauk MG, Rankin SC, Lok JB, Greenberg RM and Bau HH: Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection. Clin Chem. 63:714–722. 2017. View Article : Google Scholar : PubMed/NCBI | |
El-Tholoth M, Bau HH and Song J: A Single and Two-Stage, Closed-Tube, Molecular Test for the 2019 Novel Coronavirus (COVID-19) at Home, Clinic, and Points of Entry. ChemRxiv. View Article : Google Scholar | |
Smithgall MC, Scherberkova I, Whittier S and Green DA: Comparison of Cepheid Xpert Xpress and Abbott ID Now to Roche cobas for the Rapid Detection of SARS-CoV-2. J Clin Virol. 128:1044282020. View Article : Google Scholar : PubMed/NCBI | |
Rhoads DD, Cherian SS, Roman K, Stempak LM, Schmotzer CL and Sadri N: Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19. J Clin Microbiol. 58:e00760–e20. 2020. View Article : Google Scholar : PubMed/NCBI | |
Basu A, Zinger T, Inglima K, Woo KM, Atie O, Yurasits L, See B and Aguero-Rosenfeld ME: Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution. J Clin Microbiol. 58:e01136–e20. 2020. View Article : Google Scholar : PubMed/NCBI | |
Westermann L, Neubauer B and Köttgen M: Nobel Prize 2020 in Chemistry honors CRISPR: A tool for rewriting the code of life. Pflugers Arch. 473:1–2. 2021. View Article : Google Scholar : | |
Doudna JA and Charpentier E: Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 346:12580962014. View Article : Google Scholar : PubMed/NCBI | |
Pickar-Oliver A and Gersbach CA: The next generation of CRISPR-Cas technologies and applications. Nat Rev Mol Cell Biol. 20:490–507. 2019. View Article : Google Scholar : PubMed/NCBI | |
Broughton JP, Deng X, Yu G, Fasching CL, Singh J, Streithorst J, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv. https://doi.org/10.1101/2020.03.06.20032334. | |
Javalkote VS, Kancharla N, Bhadra B, Shukla M, Soni B, Goodin M, Bandyopadhyay A and Dasgupta S: CRISPR-based assays for rapid detection of SARS-CoV-2. Methods. Oct 9–2020.Epub ahead of print. View Article : Google Scholar : PubMed/NCBI | |
van Dongen JE, Berendsen JTW, Steenbergen RDM, Wolthuis RMF, Eijkel JCT and Segerink LI: Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities. Biosens Bioelectron. 166:1124452020. View Article : Google Scholar : PubMed/NCBI | |
Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO and Zhang F: SHERLOCK: Nucleic acid detection with CRISPR nucleases. Nat Protoc. 14:2986–3012. 2019. View Article : Google Scholar : PubMed/NCBI | |
Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu X, Li J, Walker BD, et al: Point-of-care testing for COVID-19 using SHERLOCK diagnostics. medRxiv. View Article : Google Scholar | |
Food and Drug Administration (FDA): Sherlock™ CRISPR SARS-CoV-2 kit - Instructions for use. https://www.fda.gov/media/137746/download. Accessed February 2, 2021. | |
Hou T, Zeng W, Yang M, Chen W, Ren L, Ai J, Wu J, Liao Y, Gou X, Li Y, et al: Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. PLoS Pathog. 16:e10087052020. View Article : Google Scholar | |
Curti L, Pereyra-Bonnet F and Gimenez CA: An Ultrasensitive, Rapid, and Portable Coronavirus SARS-CoV-2 Sequence Detection Method Based on CRISPR-Cas12. bioRxiv. https://doi.org/10.1101/2020.02.29.971127. | |
Salipante SJ and Jerome KR: Digital PCR-An Emerging Technology with Broad Applications in Microbiology. Clin Chem. 66:117–123. 2020. View Article : Google Scholar | |
Falzone L, Musso N, Gattuso G, Bongiorno D, Palermo CI, Scalia G, Libra M and Stefani S: Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection. Int J Mol Med. 46:957–964. 2020. View Article : Google Scholar : PubMed/NCBI | |
Falzone L, Gattuso G, Lombardo C, Lupo G, Grillo CM, Spandidos DA, Libra M and Salmeri M: Droplet digital PCR for the detection and monitoring of Legionella pneumophila. Int J Mol Med. 46:1777–1782. 2020.PubMed/NCBI | |
Crimi S, Falzone L, Gattuso G, Grillo CM, Candido S, Bianchi A and Libra M: Droplet Digital PCR Analysis of Liquid Biopsy Samples Unveils the Diagnostic Role of hsa-miR-133a-3p and hsa-miR-375-3p in Oral Cancer. Biology (Basel). 9:3792020. | |
Salemi R, Falzone L, Madonna G, Polesel J, Cinà D, Mallardo D, Ascierto PA, Libra M and Candido S: MMP-9 as a Candidate Marker of Response to BRAF Inhibitors in Melanoma Patients With BRAFV600E Mutation Detected in Circulating-Free DNA. Front Pharmacol. 9:8562018. View Article : Google Scholar : | |
Battaglia R, Palini S, Vento ME, La Ferlita A, Lo Faro MJ, Caroppo E, Borzì P, Falzone L, Barbagallo D, Ragusa M, et al: Identification of extracellular vesicles and characterization of miRNA expression profiles in human blastocoel fluid. Sci Rep. 9:842019. View Article : Google Scholar : PubMed/NCBI | |
Bizouarn F: Clinical applications using digital PCR. Methods Mol Biol. 1160:189–214. 2014. View Article : Google Scholar : PubMed/NCBI | |
Olmedillas-López S, García-Arranz M and García-Olmo D: Current and Emerging Applications of Droplet Digital PCR in Oncology. Mol Diagn Ther. 21:493–510. 2017. View Article : Google Scholar : PubMed/NCBI | |
Denis JA, Nectoux J, Lamy PJ, Rouillac Le Sciellour C, Guermouche H, Alary AS, Kosmider O, Sarafan-Vasseur N, Jovelet C, Busser B, et al: Development of digital PCR molecular tests for clinical practice: Principles, practical implementation and recommendations. Ann Biol Clin (Paris). 76:505–523. 2018. | |
Pinheiro LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, Bhat S and Emslie KR: Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification. Anal Chem. 84:1003–1011. 2012. View Article : Google Scholar : | |
Alteri C, Cento V, Antonello M, Colagrossi L, Merli M, Ughi N, Renica S, Matarazzo E, Di Ruscio F, Tartaglione L, et al: Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients. PLoS One. 15:e02363112020. View Article : Google Scholar : PubMed/NCBI | |
Suo T, Liu X, Feng J, Guo M, Hu W, Guo D, Ullah H, Yang Y, Zhang Q, Wang X, et al: ddPCR: A more accurate tool for SARS-CoV-2 detection in low viral load specimens. Emerg Microbes Infect. 9:1259–1268. 2020. View Article : Google Scholar : PubMed/NCBI | |
Liu C, Shi Q, Peng M, Lu R, Li H, Cai Y, Chen J, Xu J and Shen B: Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients. Aging (any NY). 12:20997–21003. 2020. View Article : Google Scholar | |
Vasudevan HN, Xu P, Servellita V, Miller S, Liu L, Gopez A, Chiu CY and Abate AR: Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification. Sci Rep. 11:7802021. View Article : Google Scholar : PubMed/NCBI |