Open Access

Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats

  • Authors:
    • Changju Hou
    • Xuepeng Wang
    • Wu Jiang
    • Zhenyu Bian
    • Liulong Zhu
    • Maoqiang Li
  • View Affiliations

  • Published online on: July 2, 2021     https://doi.org/10.3892/ijmm.2021.4995
  • Article Number: 162
  • Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoporotic fracture healing is a complex clinical issue. The present study was conducted to investigate the repair properties of 11R‑VIVIT on osteoporotic fractures and to examine the potential effects of 11R‑VIVIT on osteoporotic bone marrow‑derived mesenchymal stem cells (BMSCs), A rat model of osteoporotic femoral fracture was established, and the effects of the daily local injection of 11R‑VIVIT or saline on fracture repairing were evaluated by micro‑CT scans and H&E staining. Moreover, BMSCs from osteoporotic rats were treated with 11R‑VIVIT, and the osteogenic and adipogenic differentiation of BMSCs was evaluated. The results revealed that 11R‑VIVIT promoted bone formation and increased fracture healing. In addition, 11R‑VIVIT promoted the differentiation of osteoporotic BMSCs into osteoblasts rather than adipocytes. Furthermore, mechanistic analysis revealed that 11R‑VIVIT promoted autophagy by blocking the protein kinase B (AKT)/nuclear factor of activated T‑cells (NFATc1) signaling pathway. Consistently, the activation and inhibition of autophagy using rapamycin and LY294002 confirmed the regulatory effects of 11R‑VIVIT on autophagy. On the whole, the findings of the present study demonstrate that 11R‑VIVIT promotes fracture healing in osteoporotic rats and enhances the osteogenic differentiation of osteoporotic BMSCs by dysregulating the AKT/NFATc1 signaling pathway.
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August-2021
Volume 48 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hou C, Wang X, Jiang W, Bian Z, Zhu L and Li M: Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats. Int J Mol Med 48: 162, 2021.
APA
Hou, C., Wang, X., Jiang, W., Bian, Z., Zhu, L., & Li, M. (2021). Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats. International Journal of Molecular Medicine, 48, 162. https://doi.org/10.3892/ijmm.2021.4995
MLA
Hou, C., Wang, X., Jiang, W., Bian, Z., Zhu, L., Li, M."Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats". International Journal of Molecular Medicine 48.2 (2021): 162.
Chicago
Hou, C., Wang, X., Jiang, W., Bian, Z., Zhu, L., Li, M."Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats". International Journal of Molecular Medicine 48, no. 2 (2021): 162. https://doi.org/10.3892/ijmm.2021.4995