Hydrogen sulfide attenuates cigarette smoke‑induced pyroptosis through the TLR4/NF‑κB signaling pathway

  • Authors:
    • Liang Wang
    • Jing Meng
    • Caicai Wang
    • Yuan Wang
    • Chao Yang
    • Yujing Li
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  • Published online on: March 2, 2022     https://doi.org/10.3892/ijmm.2022.5112
  • Article Number: 56
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Abstract

Pyroptosis, a type of programmed cell death mediated by caspases‑1 or ‑11, may play an important role in airway epithelial injury and airway remodeling, thereby promoting the occurrence of asthma and chronic obstructive pulmonary disease (COPD). Studies have suggested that hydrogen sulfide (H2S) plays a protective role against COPD by inhibiting the activation of the NLR family pyrin domain containing 3 (NLRP3) inflammasome. The present study established a rat model of cigarette smoke (CS)‑induced COPD to observe the effects of H2S on cell pyroptosis. A 16HBE cell model was also used to further examine the effects of H2S on the Toll‑like receptor 4 (TLR4)/NF‑κB signaling pathway is affected by, and to determine the underlying mechanisms. The results revealed that cell pyroptosis was significantly promoted in the model of CS‑induced COPD. The cellular experiments also revealed that CS induced the pyroptosis of the cells in a NLRP3/gasdermin D (GSDMD)‑dependent manner. In addition, H2S significantly attenuated the effects of CS extract (CSE) on pyroptosis, cell viability and the expression levels of pyroptosis‑related proteins, indicating that H2S inhibited pyroptosis by decreasing NLRP3 expression and promoting GSDMD activation. It was also identified that CSE activated TLR4 protein in 16HBE cells, while this was inhibited by H2S. Furthermore, TLR4 and NF‑κB overexpression significantly abolished the effects of H2S on cell pyroptosis. On the whole, the findings of the present study demonstrate the role of pyroptosis in the development of COPD and provide an experimental basis for the use of H2S and drugs targeting the TLR4/NF‑κB pathway to exert protective effects against COPD.
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May-2022
Volume 49 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang L, Meng J, Wang C, Wang Y, Yang C and Li Y: Hydrogen sulfide attenuates cigarette smoke‑induced pyroptosis through the TLR4/NF‑κB signaling pathway. Int J Mol Med 49: 56, 2022.
APA
Wang, L., Meng, J., Wang, C., Wang, Y., Yang, C., & Li, Y. (2022). Hydrogen sulfide attenuates cigarette smoke‑induced pyroptosis through the TLR4/NF‑κB signaling pathway. International Journal of Molecular Medicine, 49, 56. https://doi.org/10.3892/ijmm.2022.5112
MLA
Wang, L., Meng, J., Wang, C., Wang, Y., Yang, C., Li, Y."Hydrogen sulfide attenuates cigarette smoke‑induced pyroptosis through the TLR4/NF‑κB signaling pathway". International Journal of Molecular Medicine 49.5 (2022): 56.
Chicago
Wang, L., Meng, J., Wang, C., Wang, Y., Yang, C., Li, Y."Hydrogen sulfide attenuates cigarette smoke‑induced pyroptosis through the TLR4/NF‑κB signaling pathway". International Journal of Molecular Medicine 49, no. 5 (2022): 56. https://doi.org/10.3892/ijmm.2022.5112