Open Access

Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma

  • Authors:
    • Anna Kotulak‑Chrzaszcz
    • Agnieszka Rybarczyk
    • Jakub Klacz
    • Marcin Matuszewski
    • Zbigniew Kmiec
    • Piotr M. Wierzbicki
  • View Affiliations

  • Published online on: March 8, 2022     https://doi.org/10.3892/ijmm.2022.5114
  • Article Number: 58
  • Copyright: © Kotulak‑Chrzaszcz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive subtype of kidney cancer, with high mortality rates worldwide. The sonic hedgehog (SHH) molecular cascade is altered in various malignancies in tumorigenesis, and several SHH pathway inhibitors have been considered as potential anticancer drugs. The aim of the present study was to determine the expression profile of SHH signaling components and their target genes in ccRCC. Additionally, the present study examined the effects of SHH pathway inhibitory drugs (RU‑SKI43, cyclopamine and GLI‑antagonist 61) on cell viability, cell cycle progression, expression levels of SHH target genes and migration ability in 786‑O, ACHN and HK2 cells. The study also included paired tumor and normal samples from 62 patients with ccRCC. The mRNA levels in clinical samples and cell lines were measured via reverse transcription‑quantitative PCR. Cell viability was examined using a sulforhodamine B assay. Flow cytometry was used to investigate cell cycle progression and the migratory rate of cells was assessed using a wound healing assay. High mRNA levels of SHH, smoothened (SMO), glioma‑associated zinc finger protein (GLI)1‑3, BCL2 apoptosis regulator (BCL2), MYC proto‑oncogene (MYC), vascular endothelial growth factor A (VEGFA) and cyclin D1 (CCND1) were observed in the tumor tissues, especially in early ccRCC, according to the TNM stage or World Health Organization/International Society of Urological Pathology (ISUP) grade. High expression levels of VEGFA, as well as low CCND1 mRNA expression, were associated with short overall survival, and increased VEGFA expression was an independent prognostic factor of a poor outcome in patients with advanced ISUP grade (Cox hazard ratio test). Cyclopamine treatment was found to arrest 786‑O cells in the G2/M phase and decreased the expression levels of GLI1, BCL2, VEGFA and CCND1. RU‑SKI43 inhibited cell migration and decreased the expression levels of BCL2, MYC and CCND1 in ACHN cells. Overall, the results of the present study suggested that SHH signaling may be involved in the early development of ccRCC, and the expression levels of CCND1 and VEGFA may serve as prognostic factors of this disease. Cyclopamine and RU‑SKI43 appear to be potential anti‑renal cell carcinoma drugs; however, this hypothesis requires verification by further in vivo studies.
View Figures
View References

Related Articles

Journal Cover

May-2022
Volume 49 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kotulak‑Chrzaszcz A, Rybarczyk A, Klacz J, Matuszewski M, Kmiec Z and Wierzbicki PM: Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma. Int J Mol Med 49: 58, 2022.
APA
Kotulak‑Chrzaszcz, A., Rybarczyk, A., Klacz, J., Matuszewski, M., Kmiec, Z., & Wierzbicki, P.M. (2022). Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma. International Journal of Molecular Medicine, 49, 58. https://doi.org/10.3892/ijmm.2022.5114
MLA
Kotulak‑Chrzaszcz, A., Rybarczyk, A., Klacz, J., Matuszewski, M., Kmiec, Z., Wierzbicki, P. M."Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma". International Journal of Molecular Medicine 49.5 (2022): 58.
Chicago
Kotulak‑Chrzaszcz, A., Rybarczyk, A., Klacz, J., Matuszewski, M., Kmiec, Z., Wierzbicki, P. M."Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma". International Journal of Molecular Medicine 49, no. 5 (2022): 58. https://doi.org/10.3892/ijmm.2022.5114