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A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues

  • Authors:
    • T Mancuso
    • S Crovella
    • S Casazza
    • M Pescarolo
    • L Clerico
    • P Russo
    • S Parodi

  • View Affiliations

  • Published online on: September 1, 1997     https://doi.org/10.3892/ijo.11.3.527
  • Pages: 527-531
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Abstract

We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct bl situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.

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Journal Cover

September 1997
Volume 11 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 4.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
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Spandidos Publications style
Mancuso T, Crovella S, Casazza S, Pescarolo M, Clerico L, Russo P and Parodi S: A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. Int J Oncol 11: 527-531, 1997.
APA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., & Parodi, S. (1997). A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. International Journal of Oncology, 11, 527-531. https://doi.org/10.3892/ijo.11.3.527
MLA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11.3 (1997): 527-531.
Chicago
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11, no. 3 (1997): 527-531. https://doi.org/10.3892/ijo.11.3.527