Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.

  • Authors:
    • P T Iype
    • L E Iype
    • M Verma
    • M E Kaighn
  • View Affiliations

  • Published online on: February 1, 1998     https://doi.org/10.3892/ijo.12.2.257
  • Pages: 257-320
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Abstract

New human prostate cell lines were developed from prostatic carcinoma (BRF-41T) and BPH (BRF-55T). Primary cultures were initiated from cellular outgrowths of explanted tissues. A serum-free medium, BRFF-HPC1, was developed for growing human prostatic cancer cells. Cell strains were immortalized with pRSV-T plasmid to generate permanent cell lines that exhibited an epithelial morphology. Both cell lines expressed the epithelial cell markers, cytokeratins 8 and 18 as well as the prostatic marker, PSA, and the androgen receptor gene. They possess the H-ras, K-ras, and p53 genes. We hope that these new human prostatic cell lines will be useful as in vitro models for cancer research.

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Feb 1998
Volume 12 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Iype P, Iype L, Verma M and Kaighn M: Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.. Int J Oncol 12: 257-320, 1998.
APA
Iype, P., Iype, L., Verma, M., & Kaighn, M. (1998). Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.. International Journal of Oncology, 12, 257-320. https://doi.org/10.3892/ijo.12.2.257
MLA
Iype, P., Iype, L., Verma, M., Kaighn, M."Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.". International Journal of Oncology 12.2 (1998): 257-320.
Chicago
Iype, P., Iype, L., Verma, M., Kaighn, M."Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia.". International Journal of Oncology 12, no. 2 (1998): 257-320. https://doi.org/10.3892/ijo.12.2.257