p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.

Miyake,H; Hara,I; Gohji,K; Arakawa,S; Kamidono,S

doi:10.3892/ijo.12.2.469

p53 modulation of Fas/Apo-1 mediated apoptosis in a human renal cell carcinoma cell line.

Authors:
- H Miyake
- I Hara
- K Gohji
- S Arakawa
- S Kamidono
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Affiliations: Department of Urology, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650, Japan.
Published online on: February 1, 1998 https://doi.org/10.3892/ijo.12.2.469
Pages: 469-542

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Abstract

The mutant p53 gene was transfected into ACHN, a wild-type p53-containing human renal cell carcinoma (RCC) cell line. The colony forming efficiency in soft agar in the mutant-type p53-transfected cell line (ACHN/MP) was significantly higher than that in the vector-only transfected control cell line (ACHN/C). The anti-Fas monoclonal antibody (CH11) induced apoptosis in the ACHN/C cells in a dose-dependent manner, whereas the effect of CH11 on the ACHN/ MP cells was markedly suppressed. In addition, the cytotoxic effect of CH11 on the ACHN/MP cells was augmented by the pretreatment with interferon- , but the corresponding effect on ACHN/C cells was not. These findings suggest that Fas-mediated therapy could be a novel approach to RCC, if interferon- treatment is added according to the p53 gene status.