Apolipoprotein A-I reverse transcriptase-polymerase chain reaction analysis for detection of hematogenous colon cancer dissemination.

  • Authors:
    • N Normanno
    • A De Luca
    • A Castaldo
    • A Casamassimi
    • A Di Popolo
    • R Zarrilli
    • A Porcellini
    • A M Acquaviva
    • V E Avvedimento
    • S Pignata
  • View Affiliations

  • Published online on: September 1, 1998     https://doi.org/10.3892/ijo.13.3.443
  • Pages: 443-450
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Abstract

Detection of systemic tumor dissemination in colon carcinoma patients might be important for selection of appropriate treatment modalities. It has been previously shown that Apolipoprotein A-I (Apo A-I) is expressed in human intestinal epithelial cells, and in some human colon carcinoma cell lines. We examined the expression of Apo A-I mRNA in 14 human primary colon carcinomas by Northern blot and/or reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. An Apo A-I specific transcript was found in up to 70% of the colon carcinomas. We developed an RT-PCR assay for Apo A-I transcripts, to identify circulating carcinoma cells in the peripheral blood of colon cancer patients. The Apo A-I RT-PCR assay was optimized using limiting dilution of an Apo A-I positive cancer cell line mixed with peripheral blood from healthy donor. In this system, up to 10 colon carcinoma cells were detected in 5 ml of peripheral blood. We examined Apo A-I mRNA expression in peripheral blood samples from 4 healthy donors, 20 colon carcinoma patients, and 11 individuals with tumor disease other than colon cancer. No Apo A-I mRNA was detected in the healthy donors and in the patients without colon cancer. Two out of 10 patients with metastatic colon carcinoma were positive by this assay, whereas Apo A-I mRNA was not found in any of the blood samples from the 10 radically resected colon carcinoma patients. These data suggest that Apo A-I RT-PCR assay is a highly specific and sensitive assay, although a low number of advanced colon carcinoma patients was found to be positive.

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Sep 1998
Volume 13 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Normanno N, De Luca A, Castaldo A, Casamassimi A, Di Popolo A, Zarrilli R, Porcellini A, Acquaviva A, Avvedimento V, Pignata S, Pignata S, et al: Apolipoprotein A-I reverse transcriptase-polymerase chain reaction analysis for detection of hematogenous colon cancer dissemination.. Int J Oncol 13: 443-450, 1998.
APA
Normanno, N., De Luca, A., Castaldo, A., Casamassimi, A., Di Popolo, A., Zarrilli, R. ... Pignata, S. (1998). Apolipoprotein A-I reverse transcriptase-polymerase chain reaction analysis for detection of hematogenous colon cancer dissemination.. International Journal of Oncology, 13, 443-450. https://doi.org/10.3892/ijo.13.3.443
MLA
Normanno, N., De Luca, A., Castaldo, A., Casamassimi, A., Di Popolo, A., Zarrilli, R., Porcellini, A., Acquaviva, A., Avvedimento, V., Pignata, S."Apolipoprotein A-I reverse transcriptase-polymerase chain reaction analysis for detection of hematogenous colon cancer dissemination.". International Journal of Oncology 13.3 (1998): 443-450.
Chicago
Normanno, N., De Luca, A., Castaldo, A., Casamassimi, A., Di Popolo, A., Zarrilli, R., Porcellini, A., Acquaviva, A., Avvedimento, V., Pignata, S."Apolipoprotein A-I reverse transcriptase-polymerase chain reaction analysis for detection of hematogenous colon cancer dissemination.". International Journal of Oncology 13, no. 3 (1998): 443-450. https://doi.org/10.3892/ijo.13.3.443