Decreased E-cadherin augments β-catenin nuclear localization: Studies in breast cancer cell lines
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- Published online on: March 1, 2001 https://doi.org/10.3892/ijo.18.3.541
- Pages: 541-548
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Abstract
We showed that the YMB-1-derived breast cancer cell line YMB-S, which proliferates in suspension without aggregation, exhibits complete loss of cell-cell adhesion despite the presence of E-cadherin-catenin complex and expression of free β-catenin in the cytoplasm. Here, we describe β-catenin gene regulation, interaction with E-cadherin, immunocytochemical localization, and their relation to growth rate in the YMB-1-derived cell line YMB-A, which forms tight junctions and displays anchorage-dependent growth. YMB-A cells proliferated more slowly than YMB-S cells. E-cadherin and APC gene product expression in YMB-A cells was significantly higher than that in YMB-S cells, whereas expression of β-catenin, MUC1, and c-myc was lower in YMB-A cells than in YMB-S cells. According to immunocytochemical analysis, β-catenin in YMB-A cells displayed membranous or submembranous localization, indicating that β-catenin is mostly tethered to E-cadherin. Inhibition of E-cadherin expression in YMB-A cells by an antisense oligonucleotide did not change expression of whole cell β-catenin protein, but increased nuclear β-catenin protein level, c-myc expression, and cell growth rate. These results suggest that decreased expression of E-cadherin and APC and increased amount of β-catenin in YMB-S cells lead to accumulation of β-catenin in the nucleus, activate β-catenin-LEF/TCF signaling pathway, and trigger c-myc proto-oncogene expression. c-Myc overexpression in breast cancer may be related to activated Wnt independent β-catenin-LEF/TCF signaling.